Characterization of Red Blood Cells' Rheological and Physiological State Using Optical Flicker Spectroscopy

Kononenko, V. L.

doi:10.1002/9783527634286.ch7

Cited by 2 publications

(1 citation statement)

References 92 publications

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“…A number of other optical microscopy techniques capable to obtain full-field quantitative phase information have been recently developed [12,13,14,15]. A recent review about these techniques applied to red blood cells can be found in [16]. As an example, shape and membrane fluctuations of red blood cells have been measured using diffraction phase microscopy (QPM) [15,17,18], an interferometric imaging technique that provides quantitative maps of the optical paths across living cells.…”

Section: Introductionmentioning

confidence: 99%

Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy

Siman¹,

Roma²,

Amaral³

et al. 2014

Preprint

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In this paper the bright-field defocusing microscopy (DM) technique is presented. DM is able to obtain quantitative information of each plane/surface of pure phase objects, as live unlabeled cells, and its application to red blood cells (RBCs) is demonstrated. Based on contrast, simple methods to obtain thickness profile and three dimensional (3D) total reconstruction of RBCs are proposed and the actual height profiles of upper and lower surface-membranes (lipid bilayer/cytoskeleton) of discocyte and stomatocyte red cells are presented as examples. In addition, using the mean square contrast fluctuation and modeling the RBC membranes fluctuations spectra as dependent of a bending modulus (κ c ), a surface tension (σ) and a confining potential (γ) term, slowly varying quantities along the cell radius, a genetic algorithm (GA) is used and the radial height fluctuations of each surface-membrane are accessed, separately. The radial behaviors of κ c , σ and γ are also obtained, allowing the discussion of physical aspects of the RBC membrane.

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Section: Introductionmentioning

confidence: 99%

Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy

Siman¹,

Roma²,

Amaral³

et al. 2014

Preprint

View full text Add to dashboard Cite

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Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy

et al. 2012

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Abstract. We present a real-time method to measure the amplitude of thermal fluctuations in biological membranes by means of a new treatment of the defocusing microscopy (DM) optical technique. This approach was also applied to study the deformation of human erythrocytes to its echinocyte structure. This was carried out by making threedimensional shape reconstructions of the cell and measuring the thermal fluctuations of its membrane, as the cell is exposed to the anti-inflammatory drug naproxen and as it recovers its original shape, when it is subsequently cleansed of the drug. The results showed biomechanical changes in the membrane even at low naproxen concentration (0.2 mM). Also, we found that when the cell recovered its original shape, the membrane properties were different compared to the nondrugged initial erythrocyte, indicating that the drug administration-recovery process is not completely reversible.

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Characterization of Red Blood Cells' Rheological and Physiological State Using Optical Flicker Spectroscopy

Cited by 2 publications

References 92 publications

Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy

Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy

Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy

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