Characterization of Rhizoctonia solani Isolates from Tobacco Fields Related to Anastomosis Groups 2-1 and BI (AG 2-1 and AG BI)

Nicoletti, Rosario; Lahoz, E.; Kanematsu, S.; Naito, Shigeo; Contillo, R.

doi:10.1046/j.1439-0434.1999.147002071.x

Cited by 16 publications

(9 citation statements)

References 23 publications

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“…Multiple AGs of R. solani are pathogenic on tobacco (Shew and Melton 1995;Nicoletti and Lahoz 1995;Gutierrez et al 1997;Nicoletti et al 1999;Zhao et al 2013). AG 1, AG 2-1, AG 2-2 and AG 4 cause root rot, stem rot or sore shin, and damping-off, while AG 2-1 and AG 3 cause target spot on leaves of tobacco (Shew and Melton 1995;Gutierrez et al 1997;Nicoletti et al 1999;Mercado Cárdenas et al 2012;Zhao et al 2013). In our study the R. solani isolates identified as AG 2-1 were obtained from plants with damping-off, target spot and sore shin symptoms.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Cárdenas

Galván

Barrera³

et al. 2015

Trop. plant pathol.

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In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse.Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.

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Section: Discussionmentioning

confidence: 99%

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Cárdenas

Galván

Barrera³

et al. 2015

Trop. plant pathol.

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“…Some researchers further pointed out the diversity of AG-2-1 isolates (Salazar et al 1999;Schneider et al 1997;Pannecoucque and Höfte 2009). Nicoletti et al (1999) reported Nt-isolates as a pathogen of leaf spot of tobacco and other rotation crops including sweet pepper in Italy. Their isolates had a high anastomosis frequency with tester isolates of AG-2 BI (formerly AG-BI; Carling et al 2002), although the morphology and thiamine requirement of the isolates were similar to AG-2-1.…”

Section: Discussionmentioning

confidence: 99%

The first report of tomato foot rot caused by Rhizoctonia solani AG-3 PT and AG-2-Nt and its host range and molecular characterization

Misawa

Kuninaga

2010

J Gen Plant Pathol

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Foot rot of mature tomato plants was found in four cities of Hokkaido, Japan, from 2004 to 2007. Six of eight isolates obtained from damaged tissues were identified as Rhizoctonia solani anastomosis group (AG)-3, and the remaining two isolates belonged to AG-2-1. We compared these isolates with nine reference isolates including the different subgroups in AG-3 (PT, TB and TM) and AG-2-Nt (pathogen of tobacco leaf spot) within AG-2-1 in terms of pathogenicity to tomato, tobacco and potato. All eight isolates caused foot rot on tomato. The six AG-3 isolates caused stem rot on young potato plants. While, all reference isolates of AG-3 PT causing stem rot of young potato plants incited foot rot on tomato. The two AG-2-1 isolates and an AG-2-Nt reference isolate caused severe leaf spot on tobacco leaves. The sequences of rDNA-ITS region and rDNA-IGS1 region of the AG-3 isolates showed high similarity to that of AG-3 PT isolates. Phylogenetic tree based on ITS and IGS1 regions of rDNA indicated that the AG-2-1 isolates from tomato formed a single clade with AG-2-Nt isolates and that they were separate from Japanese AG-2-1 isolates (culture type II). Pathogenicity tests and DNA sequence evaluation of the causal fungi revealed that the present isolates of AG-3 and AG-2-1 belonged to AG-3 PT and AG-2-Nt, respectively. This is the first report of tomato foot rot caused by R. solani in Japan.

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“…Como critério adicional para identificar os subgrupos de anastomose dos isolados multinucleados AG2, eles foram avaliados quanto ao requerimento para tiamina, conforme Nicoletti et al (1999): discos de cultivo em meio ágar-água foram retirados das margens da colônia e repicados para meio líquido Czapek-Dox (Dhingra & Sinclair, 1995), suplementado ou não com 5 mg/L de tiamina hidroclórica. Aos 10 dias de incubação, a 27 ºC, retirou-se o micélio, removeu-se o excesso de água em papelfiltro, mantido em funil de Büchman, sob vácuo, e pesou-se a massa micelial.…”

Section: Identificação Do Grupo De Anastomoseunclassified

Caracterização de isolados de Rhizoctonia spp. e identificação de novos grupos de anastomose em jardim clonal de eucalipto

et al. 2007

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Espécies de Rhizoctonia causam queima foliar em brotações de jardim clonal e podridão de estacas durante o enraizamento, que podem limitar a clonagem do eucalipto, por estaquia. Diante da importância do patógeno para a cultura e da falta de estudos sobre a diversidade de isolados, esse trabalho objetivou caracterizar isolados e relatar novos grupos de anastomose de Rhizoctonia spp. em jardim clonal de eucalipto. Os isolados obtidos nas diferentes fases de propagação por estaquia foram caracterizados quanto ao número de núcleos nas células vegetativas, agrupados segundo as características morfológicas das colônias e identificados quanto aos grupos de anastomose, incluindo auxotrofia por tiamina. Avaliou-se, também, a virulência ao eucalipto e o efeito da temperatura no crescimento micelial dos isolados. Não se detectou correlação entre os agrupamentos morfológicos e reações de anastomose. Constatou-se, também, que a população de Rhizoctonia spp., nos solos de jardins clonais, é constituída por ampla gama de isolados, predominantemente binucleados, com diferentes graus de virulência a eucalipto. Os isolados binucleados e os multinucleados, tiveram a mesma tendência de crescimento em relação à temperatura, com ótimo para a taxa de crescimento entre 25-30 ºC. Observou-se, pela primeira vez, isolados de R. solani AG2-2 IIIB e os binucleados de Rhizoctonia spp., AG-P e AG-O, como agentes etiológicos da podridão de estacas em casa de vegetação, e os isolados binucleados AG-A e AG-L em solo de jardim clonal de eucalipto.

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Characterization of Rhizoctonia solani Isolates from Tobacco Fields Related to Anastomosis Groups 2-1 and BI (AG 2-1 and AG BI)

Cited by 16 publications

References 23 publications

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

The first report of tomato foot rot caused by Rhizoctonia solani AG-3 PT and AG-2-Nt and its host range and molecular characterization

Caracterização de isolados de Rhizoctonia spp. e identificação de novos grupos de anastomose em jardim clonal de eucalipto

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