Characterization of rickettsiae in ticks in northeastern China

Liu, Huanhuan; Li, Qihong; Zhang, Xiaozhuo; Li, Zhongyu; Wang, Zedong; Song, Mingxin; Wei, Feng; Wang, Shuchao; Liu, Quan

doi:10.1186/s13071-016-1764-2

Cited by 84 publications

(75 citation statements)

References 36 publications

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“…Sequences of gene fragments in GenBank for rickettsial isolates obtained from H. longicornis in Japan, China, and South Korea have unknown pathogenicity and uncertain taxonomic statuses that are likely associated with Candidatus R. longicornii or a closely connected species [13]. The others were given provisory species names, including Candidatus Rickettsia jingxinensis from H. longicornis (1.0%, 2/204 pools) in China [28]. The two uncultured Rickettsia species were found to be the same species by phylogenetic analysis involving several genes [29].…”

Section: Discussionmentioning

confidence: 99%

Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea

2020

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Tick-borne pathogens (TBPs) are considered zoonotic re-emerging pathogens, with ticks playing important roles in their transmission and ecology. Previous studies in South Korea have examined TBPs residing in ticks; however, there is no phylogenetic information on TBPs in ticks parasitizing native Korean goat (NKG; Capra hircus coreanae). The present study assessed the prevalence, risk factors, and co-infectivity of TBPs in ticks parasitizing NKGs. In total, 107 hard ticks, including Haemaphysalis longicornis, Ixodes nipponensis, and Haemaphysalis flava, were obtained from NKGs in South Korea between 2016 and 2019. In 40 tested tick pools, genes for four TBPs, namely Coxiella-like endosymbiont (CLE, 5.0%), Candidatus Rickettsia longicornii (45.0%), Anaplasma bovis (2.5%), and Theileria luwenshuni (5.0%) were detected. Ehrlichia, Bartonella spp., and severe fever with thrombocytopenia syndrome virus were not detected. To our knowledge, this is the first study to report CLE and T. luwenshuni in H. flava ticks in South Korea. Considering the high prevalence of Candidatus R. longicornii in ticks parasitizing NKGs, there is a possibility of its transmission from ticks to animals and humans. NKG ticks might be maintenance hosts for TBPs, and we recommend evaluation of the potential public health threat posed by TBP-infected ticks.

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Section: Discussionmentioning

confidence: 99%

Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea

2020

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“…All unfed ticks were collected by sweep netting the vegetation, but the fed ticks were collected from cattle in Jinxin. All the ticks were identified to species based on morphological features and confirmed by the PCR assay targeting the 16S rRNA of tick as described elsewhere (Liu, Li, Zhang, et al., ).…”

Section: Methodsmentioning

confidence: 99%

Isolation and genomic characterization of lymphocytic choriomeningitis virus in ticks from northeastern China

Zhang

Huang

et al. 2018

Transbound Emerg Dis

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Lymphocytic choriomeningitis virus (LCMV) is a rodent-borne arenavirus that is considered a neglected cause of neurologic diseases in humans. In this study, we described genomic characterization of newly isolated LCMVs in Haemaphysalis longicornis, Dermacentor nuttalli, Dermacentor silvarum and Ixodes persulcatus in Jilin Province, northeastern China. The complete sequences of the small (S) and large (L) segments of LCMVs in ticks contained 3,375 and 7,235-7,241 nucleotides, respectively. Sequence comparison showed 82.1%-86.0% identity of S segment with other lineage I strains at the nucleotide level and 91.2%-97.5% at the deduced amino acid level, while a lower identity was observed in the L segment at both nucleotide (75.4%-82.2%) and amino acid (82.4%-93.4%) levels. Phylogenetic analysis grouped the tick LCMVs together with the lineage I strains, but in an isolated cluster with a high bootstrap value. Bayesian analysis indicated that the molecular evolutionary rate was estimated to be 3.3 × 10 substitutions/site/year for the S segment and 6.3 × 10 substitutions/site/year for the L segment, and the time to most recent common ancestor was 1980 and 1970 years ago, respectively, showing that tick LCMVs were predicted to originate between 1970s and 1980s. A long evolutionary history and high prevalence of LCMV in H. longicornis were found compared to other tick species. This study represented the first report on isolation of LCMV in China, showing that LCMV is circulating among ticks in Jilin Province, but the role of ticks in the epidemiology of LCMV remains to be explored.

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“…Questing adult ticks were collected by flagging vegetation in Jilin and Heilongjiang provinces, northeastern China, during April–May, 2015 (Liu et al, 2016). The obtained ticks were identified to species using the morphological method (Chen et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

“…The obtained ticks were identified to species using the morphological method (Chen et al, 2010). In total, 253 Dermacentor nuttalli , 204 Dermacentor silvarum , 412 H. concinna , 390 Haemaphysalis longicornis , and 1699 I. persulcatus were collected in northeastern China, including 206 D. nuttalli , 175 D. silvarum , 244 H. longicornis , and 393 I. persulcatus from Jilin Province, and 47 D. nuttalli , 29 D. silvarum , 412 H. concinna , 146 H. longicornis , and 1276 I. persulcatus from Heilongjiang Province (Liu et al, 2016). The predominant tick species in northeastern China was I. persulcatus (58.0%), followed by H. concinna (14.0%), H. longicornis (13.0%), D. nuttalli (8.0%), D. silvarum (7.0%).…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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Characterization of rickettsiae in ticks in northeastern China

Cited by 84 publications

References 36 publications

Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea

Molecular and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Parasitizing Native Korean Goats (Capra hircus coreanae) in South Korea

Isolation and genomic characterization of lymphocytic choriomeningitis virus in ticks from northeastern China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

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