Characterization of Ripening-Related PuARP4 in Pear (Pyrus ussuriensis)

Yuan, Hui; Zhang, Lichao; Jiang, Zhongyu; Wang, Aide

doi:10.1007/s00344-017-9680-z

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…Ethylene production rate Ethylene measurements were taken every 5 days. The ethylene production rate of the fruits was measured as previously described (Yuan et al, 2017). Five fruits per sample were used for measurement and analysis.…”

Section: Measurement and Analysis Of Physiological Datamentioning

confidence: 99%

The molecular mechanism on suppression of climacteric fruit ripening with postharvest wax coating treatment via transcriptome

et al. 2022

Front. Plant Sci.

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Wax coating is an important means to maintain fruit quality and extend fruit shelf life, especially for climacteric fruits, such as apples (Malus domestica). Here, we found that wax coating could inhibit ethylene production, chlorophyll degradation, and carotenoid synthesis, but the molecular mechanism remains unclear. The regulatory mechanism of wax coating on apple fruit ripening was determined by subjecting wax-treated apple fruits to transcriptome analysis. RNA-seq revealed that 1,137 and 1,398 genes were upregulated and downregulated, respectively. These differentially expressed genes (DEGs) were shown to be related to plant hormones, such as ethylene, auxin, abscisic acid, and gibberellin, as well as genes involved in chlorophyll degradation and carotenoid biosynthesis. Moreover, we found that some genes related to the wax synthesis process also showed differential expression after the wax coating treatment. Among the DEGs obtained from RNA-seq analysis, 15 were validated by quantitative RT-PCR, confirming the results from RNA-seq analysis. RNA-seq and qRT-PCR of pear (Pyrus ussuriensis) showed similar changes after wax treatment. Our data suggest that wax coating treatment inhibits fruit ripening through ethylene synthesis and signal transduction, chlorophyll metabolism, and carotenoid synthesis pathways and that waxing inhibits endogenous wax production. These results provide new insights into the inhibition of fruit ripening by wax coating.

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Section: Measurement and Analysis Of Physiological Datamentioning

confidence: 99%

The molecular mechanism on suppression of climacteric fruit ripening with postharvest wax coating treatment via transcriptome

et al. 2022

Front. Plant Sci.

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“…The fruit rmness measurement was carried out according to Yuan et al [37] using a portable pressure tester (FT-327, Facchini, Italy) tted with an 11-mm-diameter probe. Each fruit was cut into four thin discs (approximately 2.5 cm in diameter) from opposite sides.…”

Section: Measurement Of Fruit Rmnessmentioning

confidence: 99%

Selenium Increases Fruit Quality By Reducing Ethylene Production And The Stone Cell Content In Pear (Pyrus Ussuriensis)

Yuan

Hayee

Zhao

et al. 2021

Preprint

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Background:Selenium (Se) is an essential trace element for both animals and plants. Se treatment can increase fruit Se concentration and shelf life. However, the mechanism underlying Se-delayed fruit ripening is still unclear.Results:In this research, two groups of Se (A and B treatments) were used to treat ‘Nanhong’ pear fruit. The results showed that these treatments could greatly increase the Se content but decreased the titratable acid content. Treatment A significantly decreased ethylene production, and the key genes controlling ethylene production, PuACSs and PuERF2, were inhibited by Se treatment. In addition, treatment A significantly decreased the stone cell content, and one lignin biosynthesis gene, PuC4H, was downregulated by treatment A.Concusions:Se treatment increased the Se content in pear fruit. In addition, Se decreased ethylene production and the stone cell content. Moreover, the key genes for ethylene production (PuACSs and PuERF2) and lignin biosynthesis (PuC4H) were also inhibited by Se treatment.

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