Characterization of Robust and Free-Standing 2D-Nanomembranes of UV-Polymerized Diacetylene Lipids

Hillmann, Roland; Viefhues, Martina; Goett-Zink, Lukas; Gilzer, Dominic; Hellweg, Thomas; Kottke, Tilman; Anselmetti, Dario

doi:10.1021/acs.langmuir.7b03403

Cited by 6 publications

(21 citation statements)

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“…They are characterized by large molecules, sometimes very large. These include polysaccharides [19], synthetic lipids [82], synthetic polymers [83], proteins [84], DNA [85] and RNA [86]-based membranes.…”

Section: Organic Nanomembranesmentioning

confidence: 99%

Biomimetic Nanomembranes: An Overview

Jakšić

2020

Biomimetics

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Nanomembranes are the principal building block of basically all living organisms, and without them life as we know it would not be possible. Yet in spite of their ubiquity, for a long time their artificial counterparts have mostly been overlooked in mainstream microsystem and nanosystem technologies, being a niche topic at best, instead of holding their rightful position as one of the basic structures in such systems. Synthetic biomimetic nanomembranes are essential in a vast number of seemingly disparate fields, including separation science and technology, sensing technology, environmental protection, renewable energy, process industry, life sciences and biomedicine. In this study, we review the possibilities for the synthesis of inorganic, organic and hybrid nanomembranes mimicking and in some way surpassing living structures, consider their main properties of interest, give a short overview of possible pathways for their enhancement through multifunctionalization, and summarize some of their numerous applications reported to date, with a focus on recent findings. It is our aim to stress the role of functionalized synthetic biomimetic nanomembranes within the context of modern nanoscience and nanotechnologies. We hope to highlight the importance of the topic, as well as to stress its great applicability potentials in many facets of human life.

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Section: Organic Nanomembranesmentioning

confidence: 99%

Biomimetic Nanomembranes: An Overview

Jakšić

2020

Biomimetics

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“…The planar geometry and substrate support improves the longterm stability of the bilayer. The surface sensitive techniques, such as IR, 7 Raman, 8 3 surface plasma resonance (SPR), 9 and atomic force microscopy (AFM) 10 could be employed to obtain the molecular level information about the structure and function of the bilayer. The sBLMs deposited on the metal surface, such as gold and silver, 11 could be used to investigate the effect of the transmembrane potential.…”

Section: Introductionmentioning

confidence: 99%

Water Structure in the Submembrane Region of a Floating Lipid Bilayer: The Effect of an Ion Channel Formation and the Channel Blocker

Juhaniewicz-Dębińska

Sęk

et al. 2019

Langmuir

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The structure of water in the sub-membrane region of the bilayer of DPhPC floating (fBLM) on a monolayer of 1-thio--D-glucose (-Tg) modified gold nanoparticle film was studied by the surface enhanced infrared absorption spectroscopy (SEIRAS). SEIRAS employs surface enhancement of the mean square electric field of the photon which is acting on a few molecular layers above the film of gold nanoparticles. Therefore, it is uniquely suited to probe water molecules in the submembrane region and provides unique information concerning the structure of the hydrogen bond network of water surrounding the lipid bilayer. The IR spectra indicated that water with a strong hydrogen network is separating the membrane from the gold 2 surface. This water is more ordered than water in the bulk. When alamethicin, a peptide forming ion channels is inserted into the membrane the network is only slightly loosened. The addition of amiloride-an ion channel blocker results in a significant decrease in the amount of water in the sub-membrane region. The remaining water has a significantly distorted hydrogen bonds network. This study provides unique information about the effect of the ion channel on water transport across the bilayer.The electrode potential has a relatively small effect on water structure in the submembrane region. However, the IR studies demonstrated that water is less ordered at positive transmembrane potentials. The present results provide significant insight into the nature of hydration of floating lipid bilayer on the gold electrode surface.

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“…9 The application of the established UV-photopolymerization of amphiphiles carrying diacetylene (diyne) or hexacetylene (hexayne) functional groups 10−12 to a monolayer at an air/ water interface leads to free-standing 2D nanomembranes. 13,14 We have recently characterized the material properties of micrometer-sized 2D nanomembranes from 1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (PTPE) 1 and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) 2 lipids (Figure 1) by atomic force microscopy (AFM), helium ion microscopy, and Fourier transform infrared (FTIR) spectroscopy and identified the infrared signal of the diyne group in the monolayer before conversion. 13 These promising results motivated us to more closely investigate the photopolymerization process on the monolayer level.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Further details are described elsewhere. 13,24 FTIR Absorption Spectroscopy. The germanium crystal (72 × 10 × 2 mm 3 ) was used as an internal reflection element (IRE) with 17 active reflections for ATR spectroscopy in a Gateway ATR cell (Specac).…”

Section: ■ Introductionmentioning

confidence: 99%

“…Further details are described elsewhere. 13,24 Reference Experiments. FTIR absorption spectra of tetradecane (99%, Acros Organics), trans-7-tetradecene (97%, Sigma-Aldrich) and 1-heptene (≥99%, Sigma-Aldrich) were recorded on an IFS 66/S spectrometer (Bruker Optik) equipped with an MCT detector and an ATR setup (DuraSamplIR II, Smiths) with nine active reflections.…”

Section: ■ Introductionmentioning

confidence: 99%

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Biphasic Formation of 2D Nanomembranes by Photopolymerization of Diacetylene Lipids as Revealed by Infrared Difference Spectroscopy

et al. 2019

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Two-dimensional nanomembranes are promising materials for filtration or separation by providing the basis for controlled and rapid transport between two compartments. The polymerization by UV light of diacetylene-containing lipids at an interface produces free-standing 2D nanomembranes. Here, we analyzed in situ the nanomembrane formation of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) and 1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (PTPE) on germanium using light-induced infrared difference spectroscopy with attenuated total reflection to obtain insights into the kinetics and mechanism of the polymerization process. Our interpretation is supported by atomic force microscopy and density functional theory. Formation of the polymer network is evidenced by changes in the frequency of CO stretches acting as infrared probes. However, spectral and kinetic analysis revealed a biphasic process in the monolayer. In both phases, losses in signal of CH2 stretches are observed which are not in agreement with the accepted mechanism of chain propagation for diacetylene polymerization. These signals are dominant in the second phase and are assigned to termination reactions with some contributions from intramolecular consecutive reactions. This finding now provides a spectroscopic measure for the identity and integrity of the nanomembrane complementary to microscopic analysis. We deduce that limited 2D mobility on the solid support promotes intramolecular termination, leading to smaller domains.

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Characterization of Robust and Free-Standing 2D-Nanomembranes of UV-Polymerized Diacetylene Lipids

Cited by 6 publications

References 58 publications

Biomimetic Nanomembranes: An Overview

Biomimetic Nanomembranes: An Overview

Water Structure in the Submembrane Region of a Floating Lipid Bilayer: The Effect of an Ion Channel Formation and the Channel Blocker

Biphasic Formation of 2D Nanomembranes by Photopolymerization of Diacetylene Lipids as Revealed by Infrared Difference Spectroscopy

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