Characterization of <scp>CD</scp>127<sup>−</sup> <scp>CD</scp>25<sup>++</sup> Treg from human colostrum

Cérbulo-Vázquez, Arturo; Hernández-Peláez, Graciela; Arriaga-Pizano, Lourdes; Bautista-Pérez, Paulina; Romero-Venado, Jannett; Flores‐González, Julio; Figueroa-Damián, Ricardo; Soriano-Becerril, Diana; Mancilla-Herrera, Ismael

doi:10.1111/aji.12806

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…Early literature has speculated active immunity transfer from breastmilk to neonates [ 67 ]. More recently, breastmilk was shown to be significantly enriched in regulatory T cells compared to peripheral blood [ 68 ]. This scattered literature implicates a regulation of leukocytes in breastmilk with potential outcomes in the suckling neonate.…”

Section: Discussionmentioning

confidence: 99%

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

Piñeiro-Salvador

Vázquez‐Garza²,

Cruz-Cardenas³

et al. 2022

BMC Med

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Background Breastmilk is a dynamic fluid whose initial function is to provide the most adapted nutrition to the neonate. Additional attributes have been recently ascribed to breastmilk, with the evidence of a specific microbiota and the presence of various components of the immune system, such as cytokines and leukocytes. The composition of breastmilk varies through time, according to the health status of mother and child, and altogether contributes to the future health of the infant. Obesity is a rising condition worldwide that creates a state of systemic, chronic inflammation including leukocytosis. Here, we asked whether colostrum, the milk produced within the first 48 h post-partum, would contain a distinct leukocyte composition depending on the body mass index (BMI) of the mother. Methods We collected peripheral blood and colostrum paired samples from obese (BMI > 30) and lean (BMI < 25) mothers within 48 h post-partum and applied a panel of 6 antibodies plus a viability marker to characterize 10 major leukocyte subpopulations using flow cytometry. Results The size, internal complexity, and surface expression of CD45 and CD16 of multiple leukocyte subpopulations were selectively regulated between blood and colostrum irrespective of the study groups, suggesting a generalized cell-specific phenotype alteration. In obesity, the colostrum B lymphocyte compartment was significantly reduced, and CD16+ blood monocytes had an increased CD16 expression compared to the lean group. Conclusions This is the first characterization of major leukocyte subsets in colostrum of mothers suffering from obesity and the first report of colostrum leukocyte subpopulations in Latin America. We evidence various significant alterations of most leukocyte populations between blood and colostrum and demonstrate a decreased colostrum B lymphocyte fraction in obesity. This pioneering study is a stepping stone to further investigate active immunity in human breastmilk.

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Section: Discussionmentioning

confidence: 99%

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

Piñeiro-Salvador

Vázquez‐Garza²,

Cruz-Cardenas³

et al. 2022

BMC Med

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“…Cérbulo-Vázquez et al. have demonstrated a higher frequency of CD127 − CD25 ++ cells (Treg) in colostrum than peripheral blood, showing a passive transfer of tolerance mediated by memory cells and it is positively associated with vaginal delivery ( 143 ).…”

Section: Cells Microvesicles and Molecules Found In Hbmmentioning

confidence: 99%

“…These tolerogenic cells also exhibited higher expression of CD25, CD152, CD279, and TGF-β than blood-derived Treg cells, suggesting the increased ability of colostrum-derived Treg cells to mediate immunomodulation and maternal microchimerism ( 37 , 143 ).…”

Section: Cells Microvesicles and Molecules Found In Hbmmentioning

confidence: 99%

Human Breast Milk: From Food to Active Immune Response With Disease Protection in Infants and Mothers

et al. 2022

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Breastfeeding is associated with long-term wellbeing including low risks of infectious diseases and non-communicable diseases such as asthma, cancer, autoimmune diseases and obesity during childhood. In recent years, important advances have been made in understanding the human breast milk (HBM) composition. Breast milk components such as, non-immune and immune cells and bioactive molecules, namely, cytokines/chemokines, lipids, hormones, and enzymes reportedly play many roles in breastfed newborns and in mothers, by diseases protection and shaping the immune system of the newborn. Bioactive components in HBM are also involved in tolerance and appropriate inflammatory response of breastfed infants if necessary. This review summarizes the current literature on the relationship between mother and her infant through breast milk with regard to disease protection. We will shed some light on the mechanisms underlying the roles of breast milk components in the maintenance of health of both child and mother.

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“…In our laboratory group, we describe the presence of a type of lymphocytes responsible for suppressor responses called regulatory T cells (Treg), and based on their measured characteristics, they present a memory phenotype 53 . The presence of these cells in milk suggests that the mother, in addition to transferring cells responsive to pathogens, is capable of transferring lymphocytes that help suppress responses to harmless substances potentially coming from the mother, the environment or the bacteria transferred through this route 54,55 .…”

Section: Immune Training Mother-neonate Mediated By Breastfeeding Mic...mentioning

confidence: 99%

Microchimerism and lactation: a long-lasting immunologic legacy from mother to child

Bermejo-Haro,

Maldonado-Ibarra,

Camacho-Pacheco

et al. 2024

PERE

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The interaction between mother and fetus during pregnancy has been extensively studied. One of these interactions is the microchimerism, which is characterized by a mother-fetus exchange of cells and genetic material. In addition to gestation, the mother inherits more cells through lactation, in which biocomponents (cells, antibodies, and commensal bacteria) that play an important role in the adaptation of the newborn to the environment are transferred. The consequences of this post-partum microchimerism generate direct benefits in the first months of life, preventing diseases and infections, inducing tolerance to innocuous molecules, as well as favoring the immunological training of the newborn, with specialized responses and benefits in adult life. It is noteworthy that maternal microchimerism could be interpreted as an immunological legacy in the neonate, which is lasting for some of its components, and defining in the correct development of the progeny.

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Characterization of CD127⁻ CD25⁺⁺ Treg from human colostrum

Abstract: The higher proportion of these cells with a function-associated phenotype may reflect certain tolerogenic effects of breastmilk on newborns and infants, contributing to immune system homeostasis.

Cited by 9 publications

References 46 publications

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

Human Breast Milk: From Food to Active Immune Response With Disease Protection in Infants and Mothers

Microchimerism and lactation: a long-lasting immunologic legacy from mother to child

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Characterization of CD127− CD25++ Treg from human colostrum

Abstract: The higher proportion of these cells with a function-associated phenotype may reflect certain tolerogenic effects of breastmilk on newborns and infants, contributing to immune system homeostasis.

Cited by 9 publications

References 46 publications

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

A cross-sectional study evidences regulations of leukocytes in the colostrum of mothers with obesity

Human Breast Milk: From Food to Active Immune Response With Disease Protection in Infants and Mothers

Microchimerism and lactation: a long-lasting immunologic legacy from mother to child

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Product

Resources

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Characterization of CD127⁻ CD25⁺⁺ Treg from human colostrum