Characterization of Secondary Metabolites from Rhizopus oryzae  and Its Effect on Plant Pathogens

Peeran, Mohammed Faisal; Prasad, Lakshman; Kamil, Deeba

doi:10.20546/ijcmas.2018.703.082

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Kapang Rhizopus oryzae 15 yang ditumbuhkan pada Potato Dextrose Broth (PDB) selama 21 hari menghasilkan metil euginol yang mempunyai aktivitas antibiotik, benzena dan asam lemak (Peeran et al, 2018). Metabolit sekunder metil eugenol dari R. oryzae 6975 yang diperoleh dari fermentasi padat selama 21 hari bersifat antijamur, antibakteri dan feromon (Faisal & Prasad, 2016).…”

Section: Isolasi Dan Identifikasi Rhizopus Dari Usar Daun Jatiunclassified

Aktivitas Antibakteri Metabolit Rhizopus sp. Asal Usar Daun Jati terhadap Salmonella typhi

Khikmah,

Jatiswara

2023

Scita.

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Kapang Rhizopus sp. berperan sebagai inokulum utama dalam fermentasi tempe. Inokulum dalam fermentasi tempe juga dapat diperoleh secara alami dari usar daun jati. Rhizopus sp. yang tumbuh pada kedelai akan menghasilkan metabolit yang telah dikaji sebagai antidiare yang disebabkan bakteri Salmonella typhi sebagai penyebab demam tifoid yang masih menjadi penyakit endemis di Indonesia. Penelitian ini bertujuan untuk mempelajari aktivitas antibakteri metabolit Rhizopus sp. asal usar daun jati terhadap S. typhi. Metabolit Rhizopus sp. diproduksi pada media Sabouraud Dextrose Broth (SDB) selama 5 hari. Uji antibakteri metabolit mengunakan metode difusi kertas. Isolasi dari usar daun jati memperoleh satu isolat kapang yang teridentifikasi Rhizopus sp. Hasil uji menunjukkan bahwa crude metabolit Rhizopus sp. mempunyai aktivitas antibakteri kategori sedang terhadap S. typhi.

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Section: Isolasi Dan Identifikasi Rhizopus Dari Usar Daun Jatiunclassified

Aktivitas Antibakteri Metabolit Rhizopus sp. Asal Usar Daun Jati terhadap Salmonella typhi

Khikmah,

Jatiswara

2023

Scita.

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“…For example, in Endophytic fungi, Aspergillus clavatonanicus strain MJ31, isolated from the root system of Mirabalis jalaba plant and analysed their 28 volatile secondary metabolites in GC-MS study 17 . Mohammed et al reported that, the healthy leaf midrib portion of Tectona grandis tropical tree, isolated the endophytic fungi Phomopsis Species, its Secondary metabolite analysis especially in insecticidal activity 18 . Endophytic fungi Chaetomium globosum isolated from seed samples of Moringa olifera plant and undergone to its secondary metabolite analysis 19 .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Pharmacological and Growth Promoting Study of Endophytic Fungi Rhizopus delemer Isolated from Abutilon indicum (L.) Sweet.

Durairaj

Raj

2022

Int J Life Sci Pharm Res

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Endophytic fungi have been perceived as a potential source of bioactive secondary metabolites. Whereas Rhizopus as the endophytic fungi were isolated from the medicinal plant Abutilon indicum (L) sweet under the family Malvaceae found in tropical areas. This work aims to isolate and identify the fungal endophyte Rhizopus sp. from the medicinal plant Abutilon indicum at the molecular level, and the second objective is to test the Plant Growth Promoting activity like Indole Acidic Acid A, Hydrogen cyanide, Phosphate solubility (PO4), Catalase test, and it is the proof for plant endophyte association, lastly to analyse the extract of Rhizopus in phytochemical and pharmacology activity using Gas chromatography and Mass spectrometry study. The isolated fungal plant growthpromoting activity like Indole acetic acid production, Phosphate solubility assay, HCN production and Catalase enzyme synthesis was tested. As compared to IAA, PO4 solubility assay, HCN and Catalase synthesis test culture gave positive results. It described endophytic fungal within-hosts that are actively responsible against pathogens and stress tolerance conditions. Isolated fungal culture was able to synthesize catalase enzymes and HCN compounds. Rhizopus can act as a Phyto stimulator, broad-spectrum antimicrobial agent, increased stress tolerance capacity and also can increase the water availability in the nutrition supply to the host plant. In the qualitative screening test, there are about 12 phytochemicals that showed positive results. It gives the effort of the plant to act as a defensive mechanism against pathogens. Based on morphological and phylogenetic analyses, fungal species were identified: as Rhizopus delemer UICC 26. Accession no: LC514308. It is an unfamiliar mutant strain. Further GC-MS analysis revealed that the Identification of 20 bioactive compounds in the ethanolic extract of fungus Rhizopus delemer was Butane, 1,1-diethoxy-2-methyl-. 3,3-diethoxy-2-butanone. identified. The identification of bioactive chemical compounds is based on a Database in the National Institute of Standard Technology. The compound's retention time, peak area, molecular weight and molecular formula were matched with WILEY and NIST Library. It could be concluded that the isolated endophytic fungus Rhizopus delemer UICC 26 extract was prominent endophytic fungi in Abutilon indicum plant and also it enhances the plant growth-promoting activity. It was confirmed by the positive results of HCN and Catalase production test. In 12 different phytochemical compounds, and among 20 bioactive agent’s presence 9 shown our fungi has active form inside of plant part against the pathogen.

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Antagonistic Effect of Plant Growth-Promoting Fungi Against Fusarium Wilt Disease in Tomato: In vitro and In vivo Study

Attia

El-Wakil

Hashem

et al. 2022

Appl Biochem Biotechnol

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Fusarium wilt is considered one of the most destructive diseases for tomato plants. The novelty of this work was to investigate the antifungal and plant growth-promoting capabilities of some plant growth-promoting fungi (PGPF). Plant growth-promoting fungi (PGPF) improved the plant health and control plant infections. In this study, two fungal strains as PGPF were isolated and identified as Aspergillus fumigatus and Rhizopus oryzae using molecular method. The extracts of A. fumigatus and R. oryzae exhibited promising antifungal activity against F. oxysporum in vitro. Moreover, antagonistic effect of A. fumigatus and R. oryzae against F. oxysporum causing tomato wilt disease was evaluated in vivo. Disease severity and growth markers were recorded and in vitro antagonistic activity assay of the isolated A. fumigatus and R. oryzae against Fusarium oxysporum was measured. Physiological markers of defense in plant as response to stimulate systemic resistance (SR) were recorded. Our results indicated that A. fumigatus and R. oryzae decreased the percentage of disease severity by 12.5 and 37.5%, respectively. In addition, they exhibited relatively high protection percentage of 86.35 and 59.06% respectively. Fusarium wilt was declined the growth parameters, photosynthetic pigments, total soluble carbohydrate, and total soluble protein, whereas content of free proline, total phenols, and the activity of antioxidant enzymes activity increased under infection. Moreover, application of A. fumigatus and R. oryzae on infected plants successfully recovered the loss of morphological traits, photosynthetic pigment total carbohydrates, and total soluble proteins in comparison to infected control plants. PGPF strains in both non-infected and infected plants showed several responses in number and density of peroxidase (POD) and polyphenol oxidase (PPO) isozymes.

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Characterization of Secondary Metabolites from Rhizopus oryzae and Its Effect on Plant Pathogens

Cited by 4 publications

References 7 publications

Aktivitas Antibakteri Metabolit Rhizopus sp. Asal Usar Daun Jati terhadap Salmonella typhi

Aktivitas Antibakteri Metabolit Rhizopus sp. Asal Usar Daun Jati terhadap Salmonella typhi

Phytochemical Pharmacological and Growth Promoting Study of Endophytic Fungi Rhizopus delemer Isolated from Abutilon indicum (L.) Sweet.

Antagonistic Effect of Plant Growth-Promoting Fungi Against Fusarium Wilt Disease in Tomato: In vitro and In vivo Study

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