Characterization of Secondary Metabolites in Two Cousinia species

Abstract

Cousinia is one of the widespread genera of Asteraceae family. According to previous studies on some Cousinia species it was found that these species are rich of triterpenes, sesquiterpenes, flavonoids, acetylenes and steroids. According to our knowledge, there are no published reports on the chemical composition of C. iconica Hub.-Mor. and C. aintabensis Boiss. & Hausskn., thus we aimed to investigate secondary metabolites of these species. In this study, the phytochemical constituents of these species were evaluated. Seven of identified compounds were quantified. The quantitative and qualitative determination of compounds within the extracts was carried out by LC-MS/MS. Phytochemical analyses revealed the presence of flavonoids, saponins, terpenes and steroids. Preliminary examination of the mass spectrums revealed the presence of phenolic acids and derivatives and flavonoid compounds in extracts. According to quantitative analyses the main compound of C. iconica (CI) and C. aintabensis (CA) extracts was rutin with the highest contents (169.779 µg/mgextract and 161.638 µg/mgextract). Moreover, qualitative and quantitative study combined with different biological activities will shed new lights to the advanced studies.

PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF ERYNGIUM PYRAMIDALE BOISS. & HAUSSKN

“…Based on the results, the plant extract showed the highest TPC and TFC values for the microwave-assisted extraction technique [35]. In another study in 2020, Paşayeva et al revealed that the ethyl acetate extract of E. kotschyi showed the highest total phenolic (173.710 ± 1.088 mg gallic acid equivalent/g extract) and total flavonoid contents (86.978 ± 0.650 mg catechin equivalent/g extract), as well as indicating the highest antioxidant activity against the DPPH radical [36].…”