Characterization of secondary volatile profiles in Nigella sativa seeds from two different origins using accelerated solvent extraction and gas chromatography‐mass spectrometry

Abstract

The extraction and identification of bioactive compounds from herbs is of great interest. In this study, accelerated solvent extraction (ASE) technique was used to analyze the secondary volatile profiles in Nigella sativa seeds obtained from two different origins, Egypt and Bangladesh. The main extraction parameters, including extraction temperature, pressure and static extraction time, were investigated and optimized. Identification and quantification of the major constituents in nonpolar extracts (hexane) were achieved by means of GC-FID/GC-MS analysis with external standards. The two seeds showed a similar variety of chemical composition; however, the secondary volatiles profile of Bangladesh seed was higher than that of the Egyptian seed. A total of 25 compounds were identified from the ASE extract under the following optimum extraction conditions: 100°C, 1500 psi and 5 min, for extraction temperature, pressure and static time, respectively. The proposed technique can be used for the characterization of N. sativa varieties or cultivars.

Isolation of volatiles from Nigella sativa seeds using microwave‐assisted extraction: effect of whole extracts on canine and murine CYP1A

“…Nigella sativa L. is an herbaceous plant belonging to the botanical family Ranunculaceae. It has long been used as a natural food additive and remedy in many Middle Eastern and Far Eastern countries (Liu et al ., ). N .…”

Sambucus nigra Extracts Inhibit Infectious Bronchitis Virus at an Early Point during Replication

“…Studies of murine cytomegalovirus infection and hepatitis C infection lend support to the plant's antiviral potential in vivo, as well [36,37]. Additionally, N. sativa compound extracts, especially its saponins, alkaloids, and flavonols, show similarities with known antiviral chemicals [38][39][40]. Finally, S. nigra extract has successfully inhibited influenza A and B viruses in vitro and in vivo [41].…”

“…For example, N. sativa seed extracts predominantly contain saponins, glycosides, terpenoids and alkaloids [38,[63][64][65][66][67], many of which are similar to known antiviral chemicals [38][39][40]68]. On the other hand, R. rosea root extracts consist of many kaempferol, herbacetin, dihydromyricetin, and myricetin derivatives [32].…”

Fast and Ecological Liquid-Liquid Separation Method for Preparing Quinones Enriched Extract from Nigella sativa Oil

“…As reported earlier, the differences in the contents of individual components between different types of oil may be caused by different origin of N. sativa seeds. 25,26 In addition, N. sativa crude oil contains also significant amounts of triacylglycerols, sterols and phospholipids. 27 Since these compounds cannot be detected by GC-MS without previous modification, they can also be present in analyzed oil samples as well in the final extract.…”