Characterization Of Sendai Virus-Induced Human Placental Trophoblast Interferons

Aboagye-Mathiesen, George; Tóth, Ferenc; Juhl, Claus Bogh; Nørskov‐Lauritsen, Niels; Petersen, Peter M.; Zachar, Vladimír; Ebbesen, Peter

doi:10.1099/0022-1317-72-8-1871

Cited by 17 publications

(4 citation statements)

References 28 publications

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“…The finding of low and high IFN-␣ producers in the population studied is in accord with previous reports showing that type I IFNs are produced endogenously during normal human pregnancy [Lebon et al, 1982;Duc-Goiran et al, 1985;Paulesu et al, 1991], and that low/high producers of IFN exist [Bocci et al, 1985]. Even though the observation of IFN-␣ in the trophoblast could be due to both production and take up, the body of evidence shows that IFNs are produced by trophoblast cells [Bocci et al, 1985;Tóth et al, 1990;Aboagye-Mathiesen et al, 1991;Whaley et al, 1994]. There is some controversy concerning the specific trophoblast cell-producing IFN-␣ in the placenta.…”

Section: Discussionsupporting

confidence: 90%

High interferon alpha levels in placenta, maternal, and cord blood suggest a protective effect against intrauterine herpes simplex virus infection

et al. 1997

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Interferons (IFN) are produced by the placenta during pregnancy, and they can be detected in the maternal and fetal blood. Although the antiviral potential of IFNs is well established, it remains unclear whether the IFNs associated with pregnancy can prevent transplacental spread of viral infection. The present study was undertaken in order to determine the possible protective effect of placentally produced IFN-alpha on fetal acquisition of herpes simplex virus (HSV). Nine mothers with a known history of genital HSV infection were studied. In five cases IFN-alpha was detected in the placenta, maternal, and fetal blood, whereas in three cases IFN-alpha could not be detected. in the remaining case, IFN-alpha was found only in the maternal blood. As corroborated by the serological evidence of early HSV infection in the cord blood, the single case of vertical HSV transmission was observed in the group of IFN nonproducers. Furthermore, virus transmission did not occur in cases where IFN-alpha was present in the placenta and simultaneously in the maternal and fetal circulations. Thus, the present data indicate that high levels of IFN during pregnancy may protect the fetus from acquiring a possibly fatal intrauterine HSV infection.

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Section: Discussionsupporting

confidence: 90%

High interferon alpha levels in placenta, maternal, and cord blood suggest a protective effect against intrauterine herpes simplex virus infection

et al. 1997

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“…The ability of trophoblast IFNs to suppress T-and B-lymphocyte proliferation may be one of the factors contributing to the immunosuppression at the feto-placental unit so that maternal immune responses do not destroy pregnancy. Furthermore, IFN-ot has 1,1994 been demonstrated to be involved in prolongation of allograft survival (12, 28) and suppression of graft-versus-host disease (3). Such regulation of the immune response by trophoblast IFNs may also be important in preventing the rejection of the allogenic fetus.…”

Section: Resultsmentioning

confidence: 99%

“…{ 1-[(cPm cells without IFN -cPm cells with IFN)/ cPm cells without IFNI} x 100 (1) Cell viability. In all experiments the viability of the cells was determined by the trypan blue dye exclusion test.…”

Section: Methodsmentioning

confidence: 99%

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Production of interferons in human placental trophoblast subpopulations and their possible roles in pregnancy

Aboagye-Mathiesen

Tóth

Zdravković

et al. 1994

Clin Diagn Lab Immunol

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The human cytotrophoblasts are the first fetal cells to arise during embryogenesis and are the progenitor cells to villous (noninvasive), syncytiotrophoblast (noninvasive), "intermediate" extravillous (invasive), and "anchoring" extravillous (invasive) trophoblast subpopulations. These trophoblast subpopulations were isolated from first- and third-trimester placentae and were stimulated with Sendai virus, granulocyte-macrophage colony-stimulating factors (GM-CSF), and platelet-derived growth factor (PDGF) to produce interferons (IFNs). GM-CSF and PDGF induced very low levels of IFN in first-trimester extravillous and villous trophoblast subpopulations. Highly proliferating and invasive intermediate extravillous trophoblast cultures produced five- to eightfold more IFNs than villous trophoblast cultures and two- to fivefold more IFN than the syncytiotrophoblast cultures when stimulated with Sendai virus. Syncytiotrophoblast cultures produced higher levels of IFNs (up to twofold) than villous trophoblast cultures when stimulated with the same virus. Pretreatment of first-trimester extravillous and villous trophoblast cultures with GM-CSF and PDGF followed by infection with Sendai virus resulted in greater IFN production than when the cultures were stimulated with virus alone. The levels of IFN produced were dependent on the type of trophoblast, the type of inducer, and the stage of differentiation of the trophoblasts. The purified trophoblast IFNs have potent antiviral activities when assayed on human amniotic WISH cells, and they inhibited proliferation of normal trophoblasts and trophoblast-derived malignant cells in vitro without any toxicity. Furthermore, the trophoblast IFNs activated NK cell activity and suppressed mitogen-stimulated lymphocyte proliferation at concentrations of between 10 and 1,000 IU/ml. The possible functions of the trophoblast IFNs during pregnancy are discussed with respect to human placental and fetal protection and development.

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Pregnancy Derived Compounds that Control Proliferation

Barnea¹,

Aboagye-Mathiesen²,

Hatch³

et al. 2001

Cancer and Pregnancy

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Characterization Of Sendai Virus-Induced Human Placental Trophoblast Interferons

Cited by 17 publications

References 28 publications

High interferon alpha levels in placenta, maternal, and cord blood suggest a protective effect against intrauterine herpes simplex virus infection

High interferon alpha levels in placenta, maternal, and cord blood suggest a protective effect against intrauterine herpes simplex virus infection

Production of interferons in human placental trophoblast subpopulations and their possible roles in pregnancy

Pregnancy Derived Compounds that Control Proliferation

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