Characterization of serum levels of testosterone and corticosterone in a blast and amputation rat model of heterotopic ossification

Qin, Mack Y.; Atwood, Rex E.; Ketchum, William A.; Kaba, Stephen A.; Bradley, Matthew John

doi:10.1016/j.mce.2020.110799

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Enzyme‐based immunoassay method provides determination of small amount of hormones in body fluids. ELISA technique was used to measure serum levels of Tes (Qin et al., 2020). Also, this procedure was used to measure the serum levels of NO according to the routine protocol (Tian et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

Genetic, biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats

et al. 2021

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One of the most critical side effects of anti-cancer drugs is disruption in spermatogenesis. Paclitaxel (PTX) is the most commonly used drug in chemotherapy for various cancers, including breast, ovarian and prostate (Semet et al., 2017). This herbal alkaloid-based drug prevents replication and metastasis of tumours through destruction of the cellular cytoskeleton (Yang & Horwitz, 2017). As testes are susceptible organs to chemotherapeutic drugs, these agents can reduce the quality of sperm chromatin with adverse effects on spermatogenesis process (Semet et al., 2017). Since the past, herbal medications have been widely used for disease treatments. Nigella sativa is a plant of the Ranunculaceae family with many medicinal applications in diseases treatment, including gastrointestinal, respiratory and renal disorders (Yimer et al., 2019). Medical properties of Nigella sativa are attributed to quinone compounds. Among these,

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Section: Methodsmentioning

confidence: 99%

Genetic, biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats

et al. 2021

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“…9,12 A persuasive evidence was that the incidence of HO after blast injury then amputation (Am) was more than 65% while normal blunt Am or blast overpressure alone failed to result in HO. 5,13 Reasonable hypothesis was that HO formation following concomitant bone injury required additional muscle injury and muscle injury would induce HO following concomitant bone injury.…”

Section: Introductionmentioning

confidence: 99%

The development of a mouse model to investigate the formation of heterotopic ossification

Cao

Xiang

et al. 2023

J Orthop Surg (Hong Kong)

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Background Muscle injury and concomitant bone injury are important drivers to induce heterotopic ossification (HO). However, the related roles of muscle and concomitant bone injury in HO formation are still unclear. This study aims to develop a mouse model through the combination of hindlimb amputation (Am) and cardiotoxin (CTX) injection to investigate the mechanism of HO formation. Method The mice were randomly divided into Am group (Am of right hindlimb, n = 12), CTX group (CTX injection in the calf muscle of left hindlimb, n = 12) and Am + CTX group (the combination of Am of right hindlimb and CTX injection of left hindlimb, n = 18). MicroCT was used to evaluate the incidence of HO. Histology was used to investigate the progression of HO. Results The MicroCT showed that only Am or CTX injection failed to induce HO while the combination of Am and CTX injection successfully induced HO. The incidence of HO was significant in Am + CTX group on day 7 (0% vs 0% vs 83.3%, p = 0.001) and day 14 (0% vs 0% vs 83.3%, p = 0.048). HO was located on the left hindlimb where CTX was injected. Moreover, the bone volume and bone density on day 14 were higher than those on day 7 in Am + CTX group. Histology revealed the evidence of calcification and expression of osteogenic markers in calcification sites in Am + CTX group. Conclusion In summary, the combination of Am and CTX injection could successfully induce dystrophic calcification/HO, which occurs in the location of muscle injury.

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A review of the biomarkers and in vivo models for the diagnosis and treatment of heterotopic ossification following blast and trauma-induced injuries

Kazezian

Bull

2021

Bone

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Characterization of serum levels of testosterone and corticosterone in a blast and amputation rat model of heterotopic ossification

Cited by 5 publications

References 19 publications

Genetic, biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats

Genetic, biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats

The development of a mouse model to investigate the formation of heterotopic ossification

A review of the biomarkers and in vivo models for the diagnosis and treatment of heterotopic ossification following blast and trauma-induced injuries

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