Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens

Microbial protein display technologies have enabled directed molecular evolution of binding and stability properties in numerous protein systems. In particular, dramatic improvements to antibody binding affinity and kinetics have been accomplished using these tools in recent years. Examples of successful application of display technologies to other immunological proteins have been limited to date. Herein, we describe the expression of human class II major histocompatibility complex allele (MHCII) HLA-DR4 on the surface of Saccharomyces cerevisiae as a noncovalently associated heterodimer. The yeast-displayed MHCII is fully native as assessed by binding of conformationally specific monoclonal antibodies; failure of antibodies specific for empty HLA-DR4 to bind yeast-displayed protein indicates antigenic peptide is bound. This report represents the first example of a noncovalent protein dimer displayed on yeast and of successful display of wildtype MHCII. Results further point to the potential for using yeast surface display for engineering and analyzing the antigen binding properties of MHCII.

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Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide

Boder

Bill

Nields

et al. 2005

Biotechnol. Bioeng.

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Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins

1994

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Four of the tetraspans family antigens expressed in B cells, CD37, CD53, TAPA-1, and R2/C33, as well as at least two other molecules, CD19 and CD21, coprecipitate with DR antigens from mild detergent lysates of human B-cell lines and tonsillar B cells. Coprecipitation and preclearing experiments indicate the existence of large multicomponent complexes containing jointly the seven components, although some "incomplete" complexes lacking some of the components may also exist. The complexes contain only a relatively small fraction of the total cellular pool of relevant molecules. The existence of these "tetraspans-DR complexes" may be related to the previously reported antiproliferative and signaling effects of antibodies against most of their components.

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Monoclonal antibodies against human α-fetoprotein exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

Stefanová

Hořejšı́

Kristofová

et al. 1988

Journal of Immunological Methods

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Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens

Cited by 10 publications

References 31 publications

Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide

Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide

Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins

Monoclonal antibodies against human α-fetoprotein exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

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