Characterization of Shiga toxin producing E. coli and O157 serotype E. coli isolated in France from healthy domestic cattle

Rogerie, F; Marecat, Armelle; Gambade, Stéphanie; Dupond, Francis; Beaubois, Pierre; Lange, M.

doi:10.1016/s0168-1605(00)00422-0

Cited by 56 publications

(51 citation statements)

References 24 publications

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“…Although the screening has focused on faeces, our results differ in the low carriage rate of the stx2 gene in comparison with other authors (26), who found that 60% of STEC carrier animals were associated with the stx2 gene only and 23% with the stx1 + stx2 genes in tandem. The high frequency of stx2 gene occurrence in adult cows has been reported by few authors (1,13).…”

Section: Discussioncontrasting

confidence: 99%

Prevalence of Asymptomatic Carriers of Shiga Toxin-Producing Escherichia Coli (STEC) in Dairy Cattle Farms in the Governorate of Blida (Algeria)

Baazize-Ammi

Gassem

Derrar

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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We investigated whether dairy beef cattle raised in Algeria are Shiga toxin-producing Escherichia coli (STEC) carriers. Stx1 and stx2 genes were analysed in DNA isolated from 200 faecal samples collected from adult dairy cows from 27 randomly selected farms in Blida, North Algeria, after amplification by PCR. Samples from 61 (30.5%) animals out of the 200 were positive and were located in 18 farms with a prevalence of 66.7%. Interestingly, no sample from any cow was positive for only the stx2 gene, while in contrast, samples from 51 cows were positive for the stx1 gene alone (83.6%) and those from 10 other cows were positive for both stx1 and stx2 genes (16.4%). It should be noted that the faecal samples infected with pathogens carrying the two genes originated from 4 out of the 18 farms that were found to be positive, with a rate of 22.2%.

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Section: Discussioncontrasting

confidence: 99%

Prevalence of Asymptomatic Carriers of Shiga Toxin-Producing Escherichia Coli (STEC) in Dairy Cattle Farms in the Governorate of Blida (Algeria)

Baazize-Ammi

Gassem

Derrar

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Most of the isolates were negative for the eae gene. In France, a study performed at the slaughterhouse indicated that 18% of faecal samples were positive for stx genes, and STEC were isolated from 7.9% of these samples [145]. In another study, however, a much higher prevalence of stx genes (70%) was observed, and STEC were isolated from more than 30% of the samples [136].…”

Section: Cattlementioning

confidence: 99%

Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission

et al. 2005

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-Enterohaemorrhagic Escherichia coli (EHEC) constitute a subset of serotypes (E. coli O157 and some other serogroups) of Shiga toxin (Stx)-producing E. coli (STEC) firmly associated with severe human illnesses like bloody diarrhoea and haemolytic uraemic syndrome. Stx production is essential but not sufficient for EHEC virulence. Most strains are capable of colonising the intestinal mucosa of the host with the "attaching and effacing" mechanism, genetically governed by a large pathogenicity island (PAI) defined as the Locus of Enterocyte Effacement. Other virulence factors carried by mobile genetic elements like PAI and plasmids have been recently described, and their role in the pathogenic process has not been fully elucidated. EHEC are zoonotic pathogens. They rarely cause disease in animals, and ruminants are recognised as their main natural reservoir. Cattle are considered to be the most important source of human infections with EHEC O157, and the ecology of the organism in cattle farming has been extensively studied. The organism has also been reported in sheep, goats, water buffalos, and deer. Pigs and poultry are not considered to be a source of EHEC and the sporadic reports may derive from accidental exposure to ruminant dejections. The epidemiology of EHEC infections has remarkably changed during the past ten years and an increasing number of unusual food vehicles have been associated with human infections. New routes of transmission have emerged, like contact with animals during farm visits and a wide variety of environment-related exposures. As for other zoonotic agents, having animals and raw products that are free from EHEC is not possible in practice. However, their occurrence can be minimised by applying high standards of hygiene in all the steps of the food production chain.

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“…However, no E. coli O157:H7 isolates lacking stx were found among all of the fall isolates, which suggests that these strains may be rare. E. coli O157:H7 isolates lacking stx have been recovered from clinical and cattle fecal samples (13,26,28,31). Although the Shiga toxins are considered to be the primary virulence factors of STEC (21), it has been suggested that O157:H7/H 2 strains do not require stx genes to cause disease (1,28).…”

Section: H7mentioning

confidence: 99%

Characterization of O157:H7 and Other Escherichia coli Isolates Recovered from Cattle Hides, Feces, and Carcasses

Barkocy-Gallagher

Arthur

Rivera-Betancourt

et al. 2004

Journal of Food Protection

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In a previous study, the seasonal prevalence was reported for stx 1 Escherichia coli O157:H7 in feces and on hides and carcasses of cattle at processing. Overall, 1,697 O157:H7 isolates have now been characterized for the incidence of (i) eae O157 , hlyA, stx 1 , and stx 2 in the recovered isolates and (ii) presumptive rough and presumptive nonmotile isolates. Seven O157:H7 isolates (0.4%) lacked stx genes, although they carried eae and hlyA. All but one of the isolates carried both eae and hlyA. Approximately two-thirds of the isolates (64% when one isolate per sample was considered) carried both stx 1 and stx 2 . E. coli O157:H7 cells that harbored both stx 1 and stx 2 were more often recovered from hides in the fall (79% of the fall hide isolates) and winter (84% of the winter hide isolates) than in the spring (53%) and summer (59%). Isolates recovered from preevisceration carcasses showed a similar but not statistically signi cant trend. Twenty-three of the 25 O157:H7 isolates carrying stx 1 but not stx 2 were recovered during summer. Fifteen presumptive rough and 117 presumptive nonmotile stx 1 O157:H7 isolates were recovered. Ten (67%) of the presumptive rough isolates were recovered during summer. Ninety-ve of the presumptive nonmotile isolates (81%) were recovered during fall. Forty-eight percent of the false-positive isolates (175 of 363) tentatively identi ed as O157:H7 were O157 1 H7 2 and lacked eae O157 , hlyA, and stx. These data suggest that in beef processing samples (i) there are minor seasonal variations in the prevalence of stx genes among E. coli O157:H7 isolates, (ii) presumptive rough and presumptive nonmotile stx 1 O157:H7 isolates are present, (iii) E. coli O157:H7 isolates lacking stx genes may be rare, and (iv) O157 1 H7 2 isolates lacking stx genes can result in many false-positive results.Escherichia coli O157:H7 is an enteric pathogen that can cause diseases ranging from mild diarrhea to hemolytic uremic syndrome, kidney failure, and death (for reviews, see (17,21)). The Shiga toxins, Stx1 and Stx2, are considered to be the primary E. coli O157:H7 virulence factors, and the cells may harbor genes that express one or both of these toxins. However, the Shiga toxins alone may not be suf cient to cause disease. Additional known virulence factors include intimin and enterohemolysin, products of the eae and hlyA genes, respectively. Other virulence factors also may be involved.E. coli O157:H7 has been declared by the U.S. Food Safety and Inspection Service to be an adulterant in ground beef and nonintact beef products (2). Cattle are considered to be the primary reservoir of E. coli O157:H7 that is associated with human disease (8). Previously, we examined the seasonal prevalence of E. coli O157:H7 in feces and on hides and carcasses at beef processing plants (4). As with disease cases (8), the prevalence of E. coli O157:H7 was * Author for correspondence. ; E-mail: gallagher@email.marc.usda.gov. † Names are necessary to report factually on available data; however, the U.S. Departm...

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Characterization of Shiga toxin producing E. coli and O157 serotype E. coli isolated in France from healthy domestic cattle

Cited by 56 publications

References 24 publications

Prevalence of Asymptomatic Carriers of Shiga Toxin-Producing Escherichia Coli (STEC) in Dairy Cattle Farms in the Governorate of Blida (Algeria)

Prevalence of Asymptomatic Carriers of Shiga Toxin-Producing Escherichia Coli (STEC) in Dairy Cattle Farms in the Governorate of Blida (Algeria)

Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission

Characterization of O157:H7 and Other Escherichia coli Isolates Recovered from Cattle Hides, Feces, and Carcasses

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