Characterization of short‐term storage of the semen ofTrachelyopterus galeatus(Siluriformes: Auchenipteridae) incorporating dimethylsulphoxide (dmso) to the diluent

Abstract

Conservation of fish gametes is critical to increasing the success of induced reproduction and is widely used in aquaculture farming . Refrigeration at temperatures close to 4°C is one of the techniques commonly adopted to preserve semen for short periods, to keep semen quality feasible for hours, days and even weeks, maintaining its fertilization capacity (Contreras et al., 2019;Shaliutina et al., 2013). For this to occur, it is necessary to use diluting solutions that provide a protective medium, while both nourishing and reducing cell metabolism of sperm during storage conditions (Silva et al., 2018).Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which

A brief review of the methodology and cryoprotectants in selected fish and mammalian species

“…Based on the data in Tables 1–4, the effects of permeable and non‐permeable cryoprotectants have been extensively studied for the cryopreservation of fish (e.g. Abed‐Elmdoust et al., 2015; Aramli, Golshahi, Nazari, Aramli, & Banan, 2015; Aramli & Nazari, 2014, 2016; Golshahi et al., 2018; Judycka et al., 2021; Lopes et al., 2022; Rahimi et al., 2015) semen. The other studies have focused on the cryopreservation of mammal semen (e.g.…”