Characterization of six clinical isolates of Chimaeribacter gen. nov., a novel genus related to the Yersiniaceae family and the three species Chimaeribacter arupi sp. nov., Chimaeribacter coloradensis sp. nov, and Chimaeribacter californicus sp. nov.

Rossi, Alessandro; Fisher, Mark A.

doi:10.1099/ijsem.0.004094

Cited by 19 publications

(9 citation statements)

References 33 publications

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“…In particular, C16: 0; C17: 0 cyclo; C14: 0 3-OH/iso-C16: 1 I; C18: 1 w7c; and C 16: 1 w7 c/C 16: 1 w6 c were detected. Overall, the fatty acid profile studied showed highest similarity to members of the genera Yersinia, Proteus and Alcaligenes, among others (Rossi and Fisher, 2020).…”

Section: Genomic and Metabolic Featuresmentioning

confidence: 93%

“…In the Gram stain Chimaeribacter arupi grown at 22°C or 35°C under aerobic atmospheric conditions presents as Gram-negative pleomorphic, non-spore-forming rods with a single cell length of 0.9 µm to 4.6 µm and a width ranging from 0.4 µm to 1.0 µm (Figure 1A). The strong variation in the size of individual bacterial cells is characteristic (Rossi and Fisher, 2020). In terms of culture conditions, Chimaeribacter arupi can be cultivated under aerobic and anaerobic conditions on Columbia sheep blood or chocolate blood agar.…”

Section: Culture and Morphologymentioning

confidence: 99%

“…The accessible genomes contain 4,124-4,481 coding genes, 1-3 CRISPR arrays as well as one prophage sequence. A carotenoid synthesis operon was described, which is probably responsible for yellow pigmentation of the colonies, analogous to some Enterobacterales with yellow pigmented colonies (Rossi and Fisher, 2020). Rossi and Fisher conducted metabolic analyses on different Chimaeribacter isolates and found that they can utilize citrate, reduce nitrate, and hydrolyze aesculin.…”

Section: Genomic and Metabolic Featuresmentioning

confidence: 99%

“…The genus Chimaeribacter with the three microbial species Chimaeribacter arupi, Chimaeribacter coloradensis, and Chimaeribacter californicus was first described and validly published in 2020 by Rossi and Fisher employed by the Associated Regional and University Pathologists (ARUP) Laboratories in Salt Lake City, Utah, USA (Rossi and Fisher, 2020). The genus designation was chosen because of the multiple characteristics typical for distinct Enterobacterales genera exhibited within a single organism.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, based on the valid publication of the genus Chimaeribacter and the non-valid publication of the genus "Nissabacter", preference should be given to the genus name Chimaeribacter and the genus name "Nissabacter" should be abandoned. The genus Chimaeribacter ("Nissabacter") belongs to the order Enterobactereales and the family Yersiniaceae, which also includes the previously described Genera Rahnella, Ewingella, Rouxiella, Yersinia, Serratia, Chania, and Samsonia (Sutra et al, 2001;Rossi and Fisher, 2020;Soutar and Stavrinides, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Chimaeribacter arupi a new member of the Yersineacea family has the characteristics of a human pathogen

Riediger,

Hoffmann,

Isberner

et al. 2023

Front. Cell. Infect. Microbiol.

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Chimaeribacter arupi (heterotypic synonym: “Nissabacter archeti”) is a facultative anaerobic, newly described Gram-negative rod and belongs to the Yersineacea family. Here, we report the case of a 19-month-old female infant patient who presented to the emergency unit with somnolence and fever. C. arupi was isolated from a positive blood culture, taken via an implanted Broviac catheter, proving a bloodstream infection by the pathogen. The objective of this study was to utilize whole genome sequencing to assess the genes encoding potential virulence associated factors, which may play a role in host tropism, tissue invasion and the subsequent stages in the pathogenesis of a bloodstream infection with C. arupi. The genome of the isolate was completely sequenced employing Illumina MiSeq and Nanopore MinION sequencing and the presumptive virulence associated factors and antimicrobial resistance genes were investigated in more detail. Additionally, we performed metabolic profiling and susceptibility testing by microdilution. The presence of predicted TcfC-like α-Pili suggests that C. arupi is highly adapted to humans as a host. It utilizes flagellar and type IV pili-mediated motility, as well as a number of γ1-pili and a σ-pilus, which may be used to facilitate biofilm formation and adherence to host epithelia. Additionally, long polar fimbriae may aid in tissue invasion. The bacterium possesses antioxidant factors, which may enable temporary survival in phagolysosomes, and a capsule that potentially provides protection from phagocytosis. It may acquire iron ions from erythrocytes through the type 6 secretion system and hemolysins. Furthermore, the isolate exhibits beta-lactamase-mediated penicillin and aminopenicillin resistance. Based on the analysis of the whole genome, we conclude that C. arupi possesses virulence factors associated with tissue invasion and may thus be a potential opportunistic pathogen of bloodstream infections.

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Section: Genomic and Metabolic Featuresmentioning

confidence: 93%

Section: Culture and Morphologymentioning

confidence: 99%

Section: Genomic and Metabolic Featuresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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