Characterization of solid UV cross-linked PEGDA for biological applications

Castro, David; Ingram, Patrick; Kodzius, Rimantas; Castells-Rufas, David; Yoon, Euisik; Foulds, Ian G.

doi:10.1109/memsys.2013.6474277

Cited by 18 publications

(13 citation statements)

References 12 publications

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“…We performed polymerase chain reaction (PCR), cell viability assay, and agglutination to confirm the biocompatible issues. The PCR compatibility of silicon, silicon dioxide, polyethylene glycol diacrylate, and other surfaces have been studied [34, 37, 38]. Though 3D printer resins [39] have been tested before for cell application using phormidium and shown not to be biocompatible, we show PCR reactions, agglutination reactions, and cell studies can be performed using some of the resins tested.…”

Section: Introductionmentioning

confidence: 95%

Compatibility analysis of 3D printer resin for biological applications

Sivashankar

Agambayev

Alamoudi

et al. 2016

Micro & Nano Letters

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The salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-onchip devices is found.

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Section: Introductionmentioning

confidence: 95%

Compatibility analysis of 3D printer resin for biological applications

Sivashankar

Agambayev

Alamoudi

et al. 2016

Micro & Nano Letters

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“…Therefore, microfluidic devices for biological fluid analysis can also be made from plastics other than PDMS, such as, cyclic olefin copolymer (COC) [11,12,13,14] or poly(methyl methacrylate) (PMMA) [15,16,17,18]. Other novel and interesting materials (for forensic chips) could be SU-8 [19,20], NOA81 [21,22], or poly(ethylene glycol) diacrylate (PEGDA) [23,24].…”

Section: Introductionmentioning

confidence: 99%

Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications

et al. 2019

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Microfluidic devices offer important benefits for forensic applications, in particular for fast tests at a crime scene. A large portion of forensic applications require microfluidic chip material to show compatibility with biochemical reactions (such as amplification reactions), and to have high transparency in the visible region and high chemical resistance. Also, preferably, manufacturing should be simple. The characteristic properties of cyclic olefin copolymer (COC) fulfills these requirements and offers new opportunities for the development of new forensic tests. In this work, the versatility of COC as material for lab-on-a-chip (LOC) systems in forensic applications has been explored by realizing two proof-of-principle devices. Chemical resistance and optical transparency were investigated for the development of an on-chip presumptive color test to indicate the presence of an illicit substance through applying absorption spectroscopy. Furthermore, the compatibility of COC with a DNA amplification reaction was verified by performing an on-chip multiple displacement amplification (MDA) reaction.

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“…It is believed that as CNC content increases, the crosslinking of the PEGDA is increasingly inhibited, leading to shorter chains and networks throughout the composite. This phenomenon induces greater strains on the longer, less mobile networks of chains when the lower CNC compositions are swelled (Castro et al, 2013). This leads to a higher chance of fracture, unlike more elastic polymers such as polyurethane, where the swelling of the composite would result in no fractures (Frost and Foster, 2019).…”

Section: Resultsmentioning

confidence: 99%

Gradient Poly(ethylene glycol) Diacrylate and Cellulose Nanocrystals Tissue Engineering Composite Scaffolds via Extrusion Bioprinting

Frost¹,

Sutliff²,

Thayer

et al. 2019

Front. Bioeng. Biotechnol.

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Bioprinting has advanced drastically in the last decade, leading to many new biomedical applications for tissue engineering and regenerative medicine. However, there are still a myriad of challenges to overcome, with vast amounts of research going into bioprinter technology, biomaterials, cell sources, vascularization, innervation, maturation, and complex 4D functionalization. Currently, stereolithographic bioprinting is the primary technique for polymer resin bioinks. However, it lacks the ability to print multiple cell types and multiple materials, control directionality of materials, and place fillers, cells, and other biological components in specific locations among the scaffolds. This study sought to create bioinks from a typical polymer resin, poly(ethylene glycol) diacrylate (PEGDA), for use in extrusion bioprinting to fabricate gradient scaffolds for complex tissue engineering applications. Bioinks were created by adding cellulose nanocrystals (CNCs) into the PEGDA resin at ratios from 95/5 to 60/40 w/w PEGDA/CNCs, in order to reach the viscosities needed for extrusion printing. The bioinks were cast, as well as printed into single-material and multiple-material (gradient) scaffolds using a CELLINK BIOX printer, and crosslinked using lithium phenyl-2,4,6-trimethylbenzoylphosphinate as the photoinitiator. Thermal and mechanical characterizations were performed on the bioinks and scaffolds using thermogravimetric analysis, rheology, and dynamic mechanical analysis. The 95/5 w/w composition lacked the required viscosity to print, while the 60/40 w/w composition displayed extreme brittleness after crosslinking, making both CNC compositions non-ideal. Therefore, only the bioink compositions of 90/10, 80/20, and 70/30 w/w were used to produce gradient scaffolds. The gradient scaffolds were printed successfully and embodied unique mechanical properties, utilizing the benefits of each composition to increase mechanical properties of the scaffold as a whole. The bioinks and gradient scaffolds successfully demonstrated tunability of their mechanical properties by varying CNC content within the bioink composition and the compositions used in the gradient scaffolds. Although stereolithographic bioprinting currently dominates the printing of PEGDA resins, extrusion bioprinting will allow for controlled directionality, cell placement, and increased complexity of materials and cell types, improving the reliability and functionality of the scaffolds for tissue engineering applications.

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Characterization of solid UV cross-linked PEGDA for biological applications

Cited by 18 publications

References 12 publications

Compatibility analysis of 3D printer resin for biological applications

Compatibility analysis of 3D printer resin for biological applications

Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications

Gradient Poly(ethylene glycol) Diacrylate and Cellulose Nanocrystals Tissue Engineering Composite Scaffolds via Extrusion Bioprinting

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