Characterization of soluble polyelectrolyte-gelatin complexes by differential size-exclusion chromatography and flow field-flow fractionation

Tan, Jessie; Harrison, Catherine; Li, J. T.; Caldwell, Karin D.

doi:10.1002/(sici)1099-0488(199802)36:3<537::aid-polb14>3.0.co;2-h

Cited by 8 publications

(4 citation statements)

References 3 publications

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“…This method has been used to determine the stoichiometries of complexes between gelatin and both NaPSS and NaPAMS. 7 The results obtained by differential SEC are in excellent agreement with those obtained by previous static light scattering for both systems. Illustrated in Figure 9 are the stoichiometry data for the gelatin/NaPSS mixtures (taken from ref.…”

Section: Stoichiometry Of Complex Formationsupporting

confidence: 85%

“…NaPAMS (100% sulfonation, M w ϭ 1.1 ϫ 10 6 and polydispersity index ϭ 1.3) was synthesized and fractionated as described elsewhere. 1,7 Type IV gelatin (pHiso ϭ 4.9, M w ϭ 2.19 ϫ 10 5 and polydispersity index ϭ 2.3) 1,7 was deionized and freeze dried. Sodium acetate, acetic acid, and sodium dodecyl sulfate (SDS) were from Fisher Scientific (Pittsburgh, PA).…”

Section: Experimental Materialsmentioning

confidence: 99%

“…Alternatively, flow fieldflow fractionation can be used to determine the size of the complex. 7 In addition, molecular weight-sensitive detectors, such as a light-scattering detector, cannot be used on this SEC system to provide molecular weights of the complexes, because the background noise from the analytes in the mobile phase is too high.…”

Section: Monitoring Complex Formationmentioning

confidence: 99%

“…Our results from differential SEC for these systems have been compared previously with those obtained from flow fieldflow fractionation and static light scattering. 1,7 This article describes in detail the analytical procedure of the differential SEC method.…”

Section: Introductionmentioning

confidence: 99%

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Differential size‐exclusion chromatography for the analysis of gelatin–polyelectrolyte complexes

Harrison¹,

Tan²

1999

J. Polym. Sci. B Polym. Phys.

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Differential size‐exclusion chromatography (SEC) is used to characterize complexes formed between gelatin and two synthetic polyelectrolytes, sodium poly(styrenesulfonate) and sodium poly(2‐acrylamido‐2‐methylpropanesulfonate). The analysis is performed under aqueous, low‐salt conditions where maximum complexation between gelatin and the polyelectrolytes occurs. The adsorption effects that are commonly encountered in conventional SEC for gelatin and other charged polymers chromatographed under these solution conditions are minimized, because the columns are constantly equilibrated with the analytes in the mobile phase. Analyte solutions of identical composition, but of higher or lower concentration than that contained in the mobile phase, are injected, resulting in positive or negative detector responses, respectively. This method can separate the complexes from individual components, and can be used to determine relative sizes and stoichiometries of the complexes as a function of both the input ratio of gelatin to polyelectrolyte and the molecular weight of the polyelectrolyte. © 1999 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 37: 275–280, 1999

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Section: Stoichiometry Of Complex Formationsupporting

confidence: 85%

Section: Experimental Materialsmentioning

confidence: 99%

Section: Monitoring Complex Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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