Characterization of Somatic Embryogenesis Receptor-Like Kinase 4 as a Negative Regulator of Leaf Senescence in Arabidopsis

Li, Xiaoxu; Ahmad, Saboor; Ali, Akhtar; Guo, Chunfang; Li, Hong; Yu, Jing; Zhang, Yan; Gao, Xiao‐Ming; Guo, Yongfeng

doi:10.3390/cells8010050

Cited by 22 publications

(15 citation statements)

References 43 publications

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“…After incubation of 2–4 days, these leaves were subjected to 4,6-diamidino-2-phenylindole (DAPI) staining to confirm the position of the nucleus. As previous studies, the confocal microscope (TCS-SP8 Leica, Wetzlar, Germany) was used to capture the fluorescence signals [53,54].…”

Section: Methodsmentioning

confidence: 99%

Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses

Guo

Ahmad

et al. 2019

Biomolecules

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The MYB proteins represent a large family of transcription factors and play important roles in development, senescence, and stress responses in plants. In the current study, 233 MYB transcription factor-encoding genes were identified and analyzed in the potato genome, including 119 R1-MYB, 112 R2R3-MYB, and two R1R2R3-MYB members. R2R3-MYB is the most abundant MYB subclass and potato R2R3-MYB members together with their Arabidopsis homologs were divided into 35 well-supported subgroups as the result of phylogenetic analyses. Analyses on gene structure and protein motif revealed that members from the same subgroup shared similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. Evolution of the potato MYB family was studied via syntenic analysis. Forty-one pairs of StMYB genes were predicted to have arisen from tandem or segmental duplication events, which played important roles in the expansion of the StMYB family. Expression profiling revealed that the StMYB genes were expressed in various tissues and several StMYB genes were identified to be induced by different stress conditions. Notably, StMYB030 was found to act as the homolog of AtMYB44 and was significantly up-regulated by salt and drought stress treatments. Furthermore, overexpression of StMYB030 in Arabidopsis enhanced salt stress tolerance of transgenic plants. The results from this study provided information for further functional analysis and for crop improvements through genetic manipulation of these StMYB genes.

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Section: Methodsmentioning

confidence: 99%

Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses

Guo

Ahmad

et al. 2019

Biomolecules

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“…Senescence is the last stage of leaf development, accompanied by the hydrolysis of a series of macromolecules and the disassembly of chloroplasts and mitochondria, which ultimately leads to leaf death ( Buchanan-Wollaston et al, 2005 ; Mao et al, 2017 ; Woo et al, 2019 ). Among them, yellowing of leaves from tip to base due to the loss of chlorophyll is the most striking marker of leaf senescence ( Guo and Gan, 2005 ; Li et al, 2019 ; Woo et al, 2019 ). During leaf senescence, nutrients released by the catabolism of macromolecular substances such as proteins, lipids, and nucleic acids are transferred to active growing organs such as new buds and developing fruits and seeds, or stored for use in the next growing season ( Guo and Gan, 2005 ; Lim et al, 2007 ; Woo et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Transcription Factor NAC075 Delays Leaf Senescence by Deterring Reactive Oxygen Species Accumulation in Arabidopsis

et al. 2021

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Leaf senescence is a highly complex genetic process that is finely tuned by multiple layers of regulation. Among them, transcriptional regulation plays a critical role in controlling the initiation and progression of leaf senescence. Here, we found that the NAC transcription factor NAC075 functions as a novel negative regulator of leaf senescence. Loss of function of NAC075 promotes leaf senescence in an age-dependent manner, whereas constitutive overexpression of NAC075 delays senescence in Arabidopsis. Transcriptome analysis revealed that transcript levels of antioxidant enzymes such as catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) are significantly suppressed in nac075 mutants compared with wild-type plants. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) analyses revealed that NAC075 directly binds the promoter of catalase 2 (CAT2). Moreover, genetic analysis showed that overexpression of CAT2 suppresses the overproduction of reactive oxygen species (ROS) and the early senescence phenotypes of nac075 mutants, suggesting that CAT2 acts downstream of NAC075 to delay leaf senescence by repressing ROS accumulation. Collectively, our findings provide a new regulatory module involving NAC075-CAT2-ROS in controlling leaf senescence in Arabidopsis.

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“…Receptor‐like protein kinases are deemed to act as important cell surface receptors and are involved in a variety of biological processes, such as aging and stress responses (Zheng et al, ). Recent research revealed that the expression of RLKs was upregulated during leaf senescence in Arabidopsis (Li et al, ). Consistent with our findings, H 2 S and rhizobia prominently enhanced the gene expression of GmRLKs in soybean leaves during senescence (Figure l).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency‐induced senescence in soybean

Zhang¹,

Zou

Lin³

et al. 2020

Plant Cell & Environment

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Hydrogen sulfide (H 2 S) is emerging as an important signalling molecule that regulates plant growth and abiotic stress responses. However, the roles of H 2 S in symbiotic nitrogen (N) assimilation and remobilization have not been characterized. Therefore, we examined how H 2 S influences the soybean (Glycine max)/rhizobia interaction in terms of symbiotic N fixation and mobilization during N deficiency-induced senescence. H 2 S enhanced biomass accumulation and delayed leaf senescence through effects on nodule numbers, leaf chlorophyll contents, leaf N resorption efficiency, and the N contents in different tissues. Moreover, grain numbers and yield were regulated by H 2 S and rhizobia, together with N accumulation in the organs, and N use efficiency. The synergistic effects of H 2 S and rhizobia were also demonstrated by effects on the enzyme activities, protein abundances, and gene expressions associated with N metabolism, and senescence-associated genes (SAGs) expression in soybeans grown under conditions of N deficiency. Taken together, these results show that H 2 S and rhizobia accelerate N assimilation and remobilization by regulation of the expression of SAGs during N deficiency-induced senescence. Thus, H 2 S enhances the vegetative and reproductive growth of soybean, presumably through interactions with rhizobia under conditions of N deficiency. K E Y W O R D S assimilation, hydrogen sulfide (H 2 S), nitrogen, remobilization, rhizobia, soybean (Glycine max)

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Characterization of Somatic Embryogenesis Receptor-Like Kinase 4 as a Negative Regulator of Leaf Senescence in Arabidopsis

Cited by 22 publications

References 43 publications

Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses

Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses

Transcription Factor NAC075 Delays Leaf Senescence by Deterring Reactive Oxygen Species Accumulation in Arabidopsis

Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency‐induced senescence in soybean

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