Characterization of Sortase A in
            <i>Lactobacillus Acidophilus</i>
            ATCC 4356 Involved in Adherence to Intestinal Cells

Wu, Zhen; Wu, Jing; Lang, Fengxuan; Cai, Zhendong; Zeng, Xiaoqun; Guo, Yuxing; Liu, Xiaotao; Pan, Daodong

doi:10.2217/fmb-2019-0219

Cited by 10 publications

(3 citation statements)

References 29 publications

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“…For protection against reactive oxygen, the strain carried thioredoxin reductase (NADPH), glutathione reductase, peptide methionine sulfoxide reductase msrA/msrB [43], thiol peroxidase, thioredoxin 1 [42], and superoxide dismutase. The genes involved in adhesion in DMST-H2 included sortase A [44], elongation factor Tu, competence protein ComGC [43], and chaperonin GroEL [45]. These findings suggest the strain's ability to adapt to gastrointestinal tract conditions.…”

Section: Probiotic Potential Of Dmst-h2mentioning

confidence: 90%

Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea

Huang

Kuang

et al. 2020

Microorganisms

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Antibiotic-associated diarrhea (AAD) is the most common side effect of antibiotics and is routinely treated with probiotics in clinical. Streptococcus thermophiles, extensively utilized for producing dairy foods, has recently been regarded as a new promising probiotic candidate. In this study, the efficacy of Streptococcus thermophiles DMST-H2 (DMST-H2) for AAD treatment in mice was investigated. DMST-H2 was isolated from Chinese traditional yogurt, proved to be non-toxic, and presented tolerance against simulated gastrointestinal conditions in vitro. Additionally, genomic analysis revealed that it possessed genes related to acid tolerance, bile salt tolerance, adhesion, oxidative stress and bacteriocin production. The animal experiment results showed that both DMST-H2 treatment and natural recovery could reduce fecal water content. Compared with spontaneous recovery, DMST-H2 accelerated the recovery of the enlarged caecum and intestinal barrier injury from AAD, and further decreased endotoxin (ET), D-lactate (D-LA) and diamine oxidase (DAO) content in serum. Moreover, pro-inflammatory cytokines (TNF-α) were reduced, while interferon-γ (IFN-γ) and anti-inflammatory cytokines (IL-10) increased after treating with DMST-H2. Furthermore, DMST-H2 better restored the structure of intestinal flora. At the phylum level, Firmicutes increased and Proteobacteria decreased. These findings indicate that DMST-H2 could promote recovery in mice with antibiotic-associated diarrhea.

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Section: Probiotic Potential Of Dmst-h2mentioning

confidence: 90%

Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea

Huang

Kuang

et al. 2020

Microorganisms

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“…srtA codes sortase A; it recognizes LPxTG sorting motifs and influences the adhesion of L. acidophilus ATCC 4356. [ 27 ]. SrtA deletion significantly decreases the surface exposure of mannose-specific adhesins; this could explain the effects on mannose-specific adhesion [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215

Kim

Lee

Kim

et al. 2022

J. Microbiol. Biotechnol.

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Gamma-aminobutyric acid (GABA) improves various physiological illnesses, including diabetes, hypertension, depression, memory lapse, and insomnia in humans. Therefore, interest in the commercial production of GABA is steadily increasing. Lactic acid bacteria (LAB) have widely been reported as a GABA producer and are safe for human consumption. In this study, GABA-producing LAB were preliminarily identified and quantified via GABase assay. The acid and bile tolerance of the L. plantarum FBT215 strain were evaluated. The one-factor-at-a-time (OFAT) strategy was applied to determine the optimal conditions for GABA production using HPLC. Response surface methodology (RSM) with Box-Behnken design was used to predict the optimum GABA production. The strain FBT215 was shown to be acid and bile tolerant. The optimization of GABA production via the OFAT strategy resulted in an average GABA concentration of 1688.65 ± 14.29 μg/ml, while it was 1812.16 ± 23.16 μg/ml when RSM was applied. In conclusion, this study provides the optimum culture conditions for GABA production by the strain FBT215 and indicates that L. plantarum FBT215 is potentially promising for commercial functional probiotics with health claims.

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“…Prevotella copri , Lactobacillus acidophilus (ATCC4356) and Streptococcus suis were cultured as described previously ( Chen et al., 2021a ; Liang et al., 2022 ; Wu et al., 2020b ). The three bacteria mentioned above were grown in the presence or absence of EO in LB medium for 2, 4, 8, 12 and 24 h. The concentration of bacteria within the broth was estimated by measuring OD at 600 nm in a spectrophotometer (Thermo Scientific, Wilmington, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Essential oils improve nursery pigs' performance and appetite via modulation of intestinal health and microbiota

Zhao,

Wang,

Chen

et al. 2024

Animal Nutrition

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Characterization of Sortase A in Lactobacillus Acidophilus ATCC 4356 Involved in Adherence to Intestinal Cells

Cited by 10 publications

References 29 publications

Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea

Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea

Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215

Essential oils improve nursery pigs' performance and appetite via modulation of intestinal health and microbiota

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