Characterization of specific random amplified polymorphic (RAPD) DNA fragments related to catechin content for early detection methods in gambier plant (Uncaria gambir (Hunter) Roxb.)

Abstract

The existence of a target DNA fragments associated with the potential high level of catechins in gambier plant was carried out preliminary using RAPD markers. Target fragment was ligated and transformed into Escherichia coli DH5α strains using pGem-T Easy vector. The purpose of this study was to obtain information of specific DNA fragments and to generate specific primer combinations associated with potentially high content of catechins. DNA sequence was successfully generated with 206 nucleotides in length. The combination of specific primers, designated as Udtg3 (5'-CAGATCCTTTGCCACACTGA-3') and Udtg4 (5'-CGTACCTGCCAA CATA ACAG -3') were successfully designed and could be applied as a diagnostic marker in detection of catechin content production of gambier plant.

Genotypic identification and catechin profiling of Uncaria gambir in West Sumatra, Indonesia

“…Gambir (Uncaria gambir (W.Hunter) Roxb.) is a medicinal plant and a highly sought-after export commodity in Indonesia (Ferita et al 2013). Belonging to the Rubiaceae family, Gambir is also cultivated in Malaysia and various other Southeast Asian regions (Munggari et al 2022).…”

“…This plant grows in West Sumatra, North Sumatra, Riau, and South Sumatra in Indonesia (Yunarto et al 2023). Traditionally, Gambir has been used for the treatment of a wide range of ailments, including gastrointestinal diseases (Syukur et al 2022), wounds, stomachaches (Fahmi et al 2023), diarrhea, and diabetes (Ferita et al 2013).…”

“…In the West Sumatra region, which stands as the largest producer of Gambir in Indonesia, four distinct genotypes of Gambir are cultivated: Udang, Riau, Mancik, and Cubadak (Ferita et al 2013). Prior studies have primarily focused on morphological identification to assess genotypic diversity.…”

DNA barcoding for the discrimination of Uncaria gambir and its closely related species using internal transcribed spacer genes

“…The ITS gene is located between 18S and 28S genes in the nrDNA repeat unit and covers the ITS1 and ITS2 regions, connected by the 5.8S rRNA gene (Bellemain et al, 2010). Previous studies have used restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) to investigate catechin molecular markers in Uncaria gambir (Istino et al, 2013).…”