Characterization of sperm cell membrane charge and selection of high-quality sperm using microfluidics in stallions

Orsolini, M. F.; Verstraete, Margo H.; Heule, Machteld van; Orellana, Daniela; Ortega, Alyssa; Meyers, Stuart A.; Dini, Pouya

doi:10.1016/j.theriogenology.2022.08.014

Cited by 5 publications

(4 citation statements)

References 52 publications

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“…Microfluidic devices handle sperm cells, oocytes, and embryos and carry out other processes in an environment similar to an in vivo environment. Several microfluidic devices have been utilized to look at sperm selection based on their ability to swim in chemical (chemotaxis) and thermal differences (thermotaxis) (Marzano et al, 2020; Orsolini et al, 2022; Oseguera‐López et al, 2019; Pinto et al, 2021). However, most of these devices are used for research and have a limited therapeutic use, and determining the optimum approach for selecting high‐quality spermatozoa is critical to enhancing ART results.…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic devices are cutting-edge technologies that employ physical and chemical principles of fluid dynamics at the submicrolitre scale in a controlled environment (Orsolini et al, 2022). Microfluidic devices handle sperm cells, oocytes, and embryos and carry out other processes in an environment similar to an in vivo environment.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfluidic devices employing chemo‐ and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation

et al. 2022

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Conventional sperm processing uses centrifugation has a negative effect on sperm parameters and DNA integrity. We designed and fabricated a novel microfluid device based on chemotaxis and thermotaxis, and compared it with the swim‐up method. Twenty normal samples with high DNA fragmentation were included. Each sample was divided into four groups: Group 1, control, Group 2: sperm selection by thermotaxis, Group 3: sperm selection by chemotaxis, and Group 4: sperm selection with thermotaxis and chemotaxis. We used cumulus cells in a microfluid device to create chemotaxis, and, two warm stages to form a temperature gradient for thermotaxis. The spermatozoa were assessed based on the concentration, motility, and fine morphology using Motile Sperm Organelle Morphology Examination, mitochondrial membrane potential (MMP), acrosome reaction (AR), and sperm DNA fragmentation. Concentration (22.40 ± 5.39 vs. 66.50 ± 19.21; p < 0.001) and DNA fragmentation (12.30 ± 3.96% vs. 17.95 ± 2.89%; p < 0.001) after selection in the chemotaxis and thermotaxis microfluid device were significantly lower than control group. The progressive motility (93.75 ± 4.39% vs. 75.55 ± 5.86%, p < 0.001), normal morphology (15.45 ± 2.50% vs. 10.35 ± 3.36, p < 0.001), MMP (97.65 ± 1.81% vs. 94 ± 3.89%, p = 0.02), and AR status (79.20 ± 5.28% vs. 31.20 ± 5.24%, p < 0.001) in the chemotaxis and thermotaxis microfluid device were significantly increased compared to control group. According to these findings, spermatozoa that have penetrated the cumulus oophorus have better morphology and motility, as well as acrosome reactivity and DNA integrity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfluidic devices employing chemo‐ and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation

et al. 2022

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“…The study of systems that can process and move very small amounts of liquid using microchannels in dimensions and on a micro scale is known as micro uidics scale (22); Micro uidics' capacity to precisely regulate and manipulate uidic microenvironments has led to its use in the isolation of motile sperm through rheotaxis (23). Recently, micro uidic advancements are increasingly acknowledged as a promising platform for reproductive clinics to optimize and enhance the execution of IVF procedures (24).…”

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confidence: 99%

Separation of sperm based on rheotaxis mechanism using microfluidic device

Torkashvand,

Shabani,

Pilehvari

et al. 2024

Preprint

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Background Assisted reproductive technologies (ARTs) face a significant hurdle in efficiently selecting the most capable sperm population from a semen sample. In this study, we have developed an innovative microfluidic device designed to utilize rheotaxis for highly efficient sperm sorting. This novel approach allows for the effective selection of high-quality sperm populations compared to other conventional methods. Method 30 normal samples from couples undergoing ICSI cycle were included in the study. The samples were analyzed and examined in the andrology unit of the Infertility Center at Fatemieh Hospital in Hamedan. Each sample were split into three distinct groups: Group 1: initial sample (Control), Group 2: Direct Swim-Up sperm selection (DSU), and Group 3: Sperm selection with rheotaxis. We used a syringe pump connected to the microfluidic device to create optimal flow in the device. The spermatozoa underwent evaluation for concentration, motility, and morphology, mitochondrial membrane potential (MMP), and sperm DNA fragmentation index (DFI). A one-way ANOVA followed by a Student's t-test was conducted to ascertain the statistical significance of observed differences. Results Concentration (7.46 ± 2.84 vs. 56.67 ± 18.27; p < 0.0001) and DNA fragmentation (2.93 ± 2.70 vs. 21.13 ± 5.27; p < 0.0001) after selection in the rheotaxis microfluidic device were significantly lower than control group. The progressive motility (98.10 ± 2.41% vs. 44.13 ± 7.06%, p < 0.0001), normal morphology (8.36 ± 1.47% vs. 5.20 ± 1.15, p < 0.0001), MMP (99.63 ± 0.71% vs. 81.13 ± 9.19%, p < 0.0001), in the rheotaxis microfluidic device experienced a noteworthy rise in comparison to the control group. Conclusion our study demonstrates the effectiveness of the rheotaxis-based microfluidic device in selecting a high-quality sperm population, showcasing improved motility, morphology, mitochondrial membrane potential, and reduced DNA fragmentation. This advancement holds promise for enhancing assisted reproductive technologies.

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Profiling of charge characteristics and effect of pH on charge dynamics in cyprinid milt

Priyadarshi,

Das,

Biswal

et al. 2025

Aquaculture

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Characterization of sperm cell membrane charge and selection of high-quality sperm using microfluidics in stallions

Cited by 5 publications

References 52 publications

Microfluidic devices employing chemo‐ and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation

Microfluidic devices employing chemo‐ and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation

Separation of sperm based on rheotaxis mechanism using microfluidic device

Profiling of charge characteristics and effect of pH on charge dynamics in cyprinid milt

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