Characterization of Spiroplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gels

Mouchès, C.; Vignault, J.C.; Tully, Joseph G.; Whitcomb, R. F.; Bové, Joseph M.

doi:10.1007/bf02603058

Cited by 93 publications

(53 citation statements)

References 8 publications

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“…Our DNA homology data and data reported elsewhere on the antigenic properties (13-16), SDS-polyacrylamide gel electrophoresis profile patterns (19,20), G+C values, and DNA restriction cleavage patterns (8) indicate that the two feline ureaplasma serogroups and at least one canine serogroup (serogroup SI) represent new species or subspecies within the genus. In an accompanying paper (6), we present further characterizations of the two feline serogroups and propose that each of the feline serogroups (serogroups SI and SII) be given species status.…”

Section: Discussionsupporting

confidence: 72%

DNA Relatedness among Established Ureaplasma Species and Unidentified Feline and Canine Serogroups

Harasawa

Stephens

Koshimizu

et al. 1990

International Journal of Systematic Bacteriology

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The levels of DNA relatedness among two unclassified feline ureaplasma serogroups, four unclassified canine ureaplasma serogroups, and the three previously established Ureuplasmu species were examined and compared. The strains examined included five feline strains representing two feline serogroups, four canine strains representing four canine serogroups, and the type strains of the three established species. Each strain representing each species or serogroup exhibited 78% or more actual DNA homology with its homologous DNA, but less than 10% DNA homology with DNAs from the heterologous strains. These findings indicate that each of these human, bovine, avian, feline, and canine strains is genomically distinct. In addition, the three previously recognized species (UreupZusma ureulyticum [human], Ureaplusmu diversum [bovine], and Ureuplusmu gallorule [avian]), which were established on the basis of phenotypic properties, were also shown to be genomically distinct. The three feline serogroup SI strains were genomically related (from 89 to 100% DNA homology) to each other but were unrelated (less than 10% DNA homology) to the feline serogroup SII strains, indicating that these two feline serogroups are also genomically distinct. Conversely, the two feline serogroup SII strains were genomically very similar (from 83 to 100% DNA homology) to each other but were unrelated (less than 10% DNA homology) to the three feline serogroup SI strains. However, canine serogroup SI strain D1M-C exhibited 73 % DNA homology with serologically distinct canine serogroup SII strain D29M, indicating that these strains representing two separate serogroups belong to the same genomic species. Our findings indicate that the two feline ureaplasma serogroups and at least one canine ureaplasma serogroup are genomically distinct and are unrelated to the previously established species and that each serogroup represents a new species or subspecies within the genus Ureaplusmu.

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Section: Discussionsupporting

confidence: 72%

DNA Relatedness among Established Ureaplasma Species and Unidentified Feline and Canine Serogroups

Harasawa

Stephens

Koshimizu

et al. 1990

International Journal of Systematic Bacteriology

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“…The number of immunoprecipitin rockets identified by crossed immunoelectrophoresis represents only a fraction (about 10 %) of the total number of polypeptides in spiroplasmas (Mouches et al, 1979). The resolution of two-dimensional gel electrophoresis (Mouches et al, 1979) is higher than that of crossed immunoelectrophoresis and while a number of the polypeptides may not elicit antibody formation in the rabbit it is unlikely that the number of rockets seen represents the total number of antigenic proteins. It is therefore possible that the tulip tree spiroplasmas and SMCA may have some relationship to S. citri.…”

Section: Discussionmentioning

confidence: 99%

Immunoelectrophoretic Separation of Spiroplasma Antigens

Archer

Townsend

1981

Microbiology

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Antigens from a number of spiroplasmas were solubilized in the neutral detergent Lubrol PX and compared by crossed immunoelectrophoresis. Samples were also run in tandem with the type species Spiroplasma citri, continuity of immunoprecipitin rockets indicating antigenic identity and serological relatedness of spiroplasmas. It was concluded that corn stunt spiroplasmas, the honeybee (BC3) spiroplasma and the tick (277F) spiroplasma were related serologically to S. citri but some flower isolates and the tick (SMCA) spiroplasma showed no obvious relationship. The serological relatedness of S. citri and the honeybee (BC3) spiroplasma was investigated in detail. These two spiroplasmas each contain a major membrane protein which differ slightly in molecular weight. The two proteins were purified under identical conditions and used to raise monospecific antisera. Using the sera obtained in this way with a modified immunoelectrophoretic system having sodium dodecyl sulphatepolyacrylamide gel electrophoresis as the first dimension separation, it was shown that the major membrane proteins of S. citri and the honeybee (BC3) spiroplasma are antigenically dissimilar. We suggest that spiralin, the major membrane protein of S . citri, and the major membrane protein of the honeybee (BC3) spiroplasma are dominant cell surface antigens, so that while these two spiroplasmas are known to be very similar, serological tests involving comparison of cell surface antigens indicate less relationship. I N T R O D U C T I O NRelatedness of spiroplasmas has been estimated by many techniques, and serology has played a major part in formulating classification schemes within the family Spiroplasmataceae (Order Mycoplasmatales, Class Mollicutes). At least four serological groups are recognized (Davis et al., 1979; Williamson et al., 1979). The Spiroplasma citri group includes sub-groups of corn stunt spiroplasmas, some honeybee spiroplasmas, including strain BC 3, and the tick (277F) spiroplasma.Serological typing gives a technically simple approximation to genotypic homology, but different serological tests indicate different levels of relatedness between spiroplasmas. Spiroplasma citri and BC3 have a 65 % DNA homology (Junca et al

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“…The difference was partial, however, in that some protein bands were common to both. In two-dimensional protein electrophoretic studies of spiroplasmas, Mouches et al (18) found a few common proteins and a significant number of homologous proteins in Spiroplasma citri and the CSS. From the protein maps, they concluded that the C S S shows definite relatedness to Spiroplasma citri, but suckling mouse cataract spiroplasma probably deserves a different species designation.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, there is no precedent for species delineation solely on this basis (14). Eventually, twodimensional gel electrophoresis of cell proteins (18) The present study was undertaken to examine the value of deoxyribonucleic acid (DNA) homology as a taxonomic tool for differentiating the CSS from Spiroplasma citri. Preparation of labeled DNA.…”

mentioning

confidence: 99%

Deoxyribonucleic Acid Relationship Between Spiroplasma citri and the Corn Stunt Spiroplasma

Rahimian

Gumpf

1980

International Journal of Systematic Bacteriology

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Spiroplasma citri and the corn stunt spiroplasma were compared by deoxyribonucleic acid reassociation. Homologies among Spiroplasma citri strains ranged from 93 to loo%, but between Spiroplasma citri and the corn stunt spiroplasma, the homology was about 68%. It is therefore concluded that the corn stunt spiroplasma and SpiropZasma citri may belong to the same species but to separate subspecies or biovars.

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Characterization of Spiroplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gels

Cited by 93 publications

References 8 publications

DNA Relatedness among Established Ureaplasma Species and Unidentified Feline and Canine Serogroups

DNA Relatedness among Established Ureaplasma Species and Unidentified Feline and Canine Serogroups

Immunoelectrophoretic Separation of Spiroplasma Antigens

Deoxyribonucleic Acid Relationship Between Spiroplasma citri and the Corn Stunt Spiroplasma

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