Characterization of ST80 Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus clone in Tunisia

Nejma, Mouna Ben; Mastouri, Maha; Jrad, Besma Bel Hadj; Nour, Mohamed

doi:10.1016/j.diagmicrobio.2008.02.010

Cited by 47 publications

(30 citation statements)

References 40 publications

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“…6,7 This clone is also known to be frequent in North African and Middle East countries such as Tunisia, Jordan, and Kuwait. 1,17,31 However, the heterogeneity of the MRSA strains isolated in this study was to some extent a surprising finding compared to the large predominance of ST80-IV in the preceding investigations. Noteworthy, the two tst1 positive ST22-MRSA-IV strains, spa type t223, appeared to be similar to the ''Gaza strain,'' the predominant MRSA clone in healthy children and their parents living in the Gaza strip.…”

Section: Discussioncontrasting

confidence: 46%

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Djoudi

Benallaoua

Aléo

et al. 2015

Microbial Drug Resistance

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Section: Discussioncontrasting

confidence: 46%

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Djoudi

Benallaoua

Aléo

et al. 2015

Microbial Drug Resistance

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“…Epidemiological data suggested that the clone may have had its origin outside Europe, as many of the European CA-MRSA cases in Scandinavia were associated with import by individuals with relations to the Middle East and Africa (24, 25). Concurrently, multiple reports showed that the European CA-MRSA clone was prevalent in North Africa and the Middle East (13, 14, 26–28). …”

Section: Introductionmentioning

confidence: 93%

“…It is notable that CC80 isolates have historically been found to be predominantly methicillin resistant, and a recent European multicenter study showed that they account for >30% of all CA-MRSA isolates (31). In contrast, CC80 isolates are rarely found in cases of S. aureus bacteremia or among healthy carriers (28, 31–37). …”

Section: Introductionmentioning

confidence: 99%

Origin and Evolution of European Community-Acquired Methicillin-Resistant Staphylococcus aureus

Stegger

Wirth

Andersen

et al. 2014

mBio

109

121

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Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was recognized in Europe and worldwide in the late 1990s. Within a decade, several genetically and geographically distinct CA-MRSA lineages carrying the small SCCmec type IV and V genetic elements and the Panton-Valentine leukocidin (PVL) emerged around the world. In Europe, the predominant CA-MRSA strain belongs to clonal complex 80 (CC80) and is resistant to kanamycin/amikacin and fusidic acid. CC80 was first reported in 1993 but was relatively rare until the late 1990s. It has since been identified throughout North Africa, the Middle East, and Europe, with recent sporadic reports in sub-Saharan Africa. While strongly associated with skin and soft tissue infections, it is rarely found among asymptomatic carriers. Methicillin-sensitive S. aureus (MSSA) CC80 strains are extremely rare except in sub-Saharan Africa. In the current study, we applied whole-genome sequencing to a global collection of both MSSA and MRSA CC80 isolates. Phylogenetic analyses strongly suggest that the European epidemic CA-MRSA lineage is derived from a PVL-positive MSSA ancestor from sub-Saharan Africa. Moreover, the tree topology suggests a single acquisition of both the SCCmec element and a plasmid encoding the fusidic acid resistance determinant. Four canonical SNPs distinguish the derived CA-MRSA lineage and include a nonsynonymous mutation in accessory gene regulator C (agrC). These changes were associated with a star-like expansion into Europe, the Middle East, and North Africa in the early 1990s, including multiple cases of cross-continent imports likely driven by human migrations.

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“…European clone ST80-IV (allelic profile 1-3-1-14-11-51-10) was first identified in the early 1990s and today is found throughout Europe, the Middle East, and Northern Africa [10], [16], [17], [18], [19], [20]. This clonal lineage is PVL-positive, belongs to agr type III, has type 8 capsular polysaccharide, and is resistant to tetracycline, streptomycin, kanamycin, and fusidic acid with a pronounced susceptibility to gentamicin [18], [19], [20].…”

Section: Introductionmentioning

confidence: 99%

Community-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 80 Type IV (CC80-MRSA-IV) Isolated from the Middle East: A Heterogeneous Expanding Clonal Lineage

Harastani

Tokajian

2014

PLoS ONE

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BackgroundThe emergence of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) has caused a change in MRSA epidemiology worldwide. In the Middle East, the persistent spread of CA-MRSA isolates that were associated with multilocus sequence type (MLST) clonal complex 80 and with staphylococcal cassette chromosome mec (SCCmec) type IV (CC80-MRSA-IV), calls for novel approaches for infection control that would limit its spread.Methodology/Principal FindingsIn this study, the epidemiology of CC80-MRSA-IV was investigated in Jordan and Lebanon retrospectively covering the period from 2000 to 2011. Ninety-four S. aureus isolates, 63 (67%) collected from Lebanon and 31 (33%) collected from Jordan were included in this study. More than half of the isolates (56%) were associated with skin and soft tissue infections (SSTIs), and 73 (78%) were Panton-Valentine Leukocidin (PVL) positive. Majority of the isolates (84%) carried the gene for exofoliative toxin d (etd), 19% had the Toxic Shock Syndrome Toxin-1 gene (tst), and seven isolates from Jordan had a rare combination being positive for both tst and PVL genes. spa typing showed the prevalence of type t044 (85%) and pulsed-field gel electrophoresis (PFGE) recognized 21 different patterns. Antimicrobial susceptibility testing showed the prevalence (36%) of a unique resistant profile, which included resistance to streptomycin, kanamycin, and fusidic acid (SKF profile).ConclusionsThe genetic diversity among the CC80 isolates observed in this study poses an additional challenge to infection control of CA-MRSA epidemics. CA-MRSA related to ST80 in the Middle East was distinguished in this study from the ones described in other countries. Genetic diversity observed, which may be due to mutations and differences in the antibiotic regimens between countries may have led to the development of heterogeneous strains. Hence, it is difficult to maintain “the European CA-MRSA clone” as a uniform clone and it is better to designate as CC80-MRSA-IV isolates.

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Characterization of ST80 Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus clone in Tunisia

Cited by 47 publications

References 40 publications

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Descriptive Epidemiology of Nasal Carriage ofStaphylococcus aureusand Methicillin-ResistantStaphylococcus aureusAmong Patients Admitted to Two Healthcare Facilities in Algeria

Origin and Evolution of European Community-Acquired Methicillin-Resistant Staphylococcus aureus

Community-Associated Methicillin-Resistant Staphylococcus aureus Clonal Complex 80 Type IV (CC80-MRSA-IV) Isolated from the Middle East: A Heterogeneous Expanding Clonal Lineage

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