Characterization of Standard Reference Material 2943, Cu-ion-doped glass, spectral correction standard for blue fluorescence

Abstract

a b s t r a c tStandard Reference Material (SRM) 2943 is a cuvette-shaped, Cu-ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance verification of steady-state fluorescence spectrometers. Properties of this standard that influence its effective use or contribute to the uncertainty in its certified emission spectrum were explored here. These properties include its photostability, absorbance, dissolution rate in water, anisotropy and temperature coefficient of fluorescence intensity. The expanded uncertainties in the certified spectrum are about 5% around the peak maximum at 446 nm, using an excitation wavelength of 330 nm. SRM 2943 can replace SRM 936a quinine sulfate dihydrate, which is no longer sold by NIST, for many applications, as it covers the same spectral range. SRM 2943 is significantly more photostable than organic dyes, but unlike the other fluorescent glass SRMs in this series, it does photodegrade gradually under lamp-based excitation.

State-of-the Art Comparability of Corrected Emission Spectra.1. Spectral Correction with Physical Transfer Standards and Spectral Fluorescence Standards by Expert Laboratories

“…Until recently, ,,,− , dye QS was the only commercial fluorescence standard supplied with a normalized certified corrected emission spectrum including wavelength-dependent uncertainties. For this reason, comparison of the study result to this universally used emission standard was a necessity.…”

“…The development of fluorescence standardization methods involves suitable instrument calibration, validation procedures, and standards for all instrument quantities and parameters that can affect the analyte-specific spectral position, spectral shape, and intensity of measured fluorescence signals. Relevant quantities include the (relative) spectral responsivity of the emission channel, the (relative) spectral irradiance of the excitation beam at the sample, and the accuracy of excitation and emission wavelengths and bandwidths. ,, For this purpose, new spectral fluorescence standards were recently developed by the National Institute of Standards and Technology (NIST; glass-based reference materials (RMs)) − and the Federal Institute for Materials Research and Testing (BAM, Germany; liquid RMs). ,, The importance of the reliable determination of the spectral responsivity of the detection channel for the comparability of emission spectra and the determination of reliable fluorescence quantum yields encouraged four National Metrology Institutes (NMIs) currently active in the area of high-precision steady-state fluorometry to evaluate the state-of-the-art comparability of corrected fluorescence emission spectra, reported here. For this purpose, different instrument calibration procedures using calibrated physical transfer standards (PTSs), such as standard (calibrated) lamps, detectors, and diffuse reflectors, and easy-to-use, certified, commercially available RMs were assessed.…”

Wave Function Engineering in CdSe/PbS Core/Shell Quantum Dots

“…The intensity of the first absorption peak was kept between 0.05 and 0.1 absorbance units for optimal signal and to minimize reabsorption of emitted light. The intensity profiles of the emission spectra were corrected using an instrument response function created using standard reference materials (see Supporting Information for details). − …”

Меры На Основе Стекол, Активированных Ионами Редкоземельных Элементов, Для Калибровки Флуоресцентных И Рамановских Спектрометров

“…В течение 2008−2013 гг. NIST были разработаны стандартные образцы на основе неорганических стекол [5][6][7][8][9], предназначенные для коррекции относительных спектров эмиссии. Каждый из этих стандартных образцов имеет достаточно широкую линию эмиссии, а в совокупности они охватывают достаточно широкий диапазон длин волн эмиссии -от 320 до 630 nm.…”