Characterization of stationary phases used in reversed-phase and hydrophobic interaction chromatography

Rippel, Géza; Alattyani, Edit; Szepesy, L.

doi:10.1016/0021-9673(94)80119-3

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…Most of the tests have been designed for reversed stationary phases. It has been shown [10] that the column parameters such as hydrophobicity and hydrophilicity strongly depend on the nature of the test substances. It is thus of fundamental importance to choose test substances that are as similar to the compounds to be separated as possible.…”

Section: Introductionmentioning

confidence: 99%

Capillary liquid chromatography as a tool for separation of hydrophobic basic drugs. Relation between tests for column characterization and real analysis

Kafková

Tesařová²,

Suchánková

et al. 2003

J of Separation Science

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Capillary liquid chromatography as a tool for separation of hydrophobic basic drugs. Relation between tests for column characterization and real analysis Two capillary columns for reversed phase (RP) capillary liquid chromatography (CLC), viz. Nucleosil 100 -5 C18 and LiChrosorb RP-select B, were characterized by the Walters test, i.e. the chromatographic test proposed for RP stationary phases. Hydrophobicity indices were determined not only in acetonitrile/water mobile phase, as proposed in the test, but they were also measured in buffered systems. This approach was used to quantify the influence of mobile phase composition on the modification of the surface of the stationary phases. In the next step, small basic compounds differing in their hydrophobicity and basicity were selected and their retention on the stationary phases in mobile phases of the same composition as used for column testing was examined. Furthermore, the retention of newly synthesized drugs, chemotherapeutics derived from thioacridine and pyridoquinoline, differing in their structures, basicity, and hydrophobicity, was also studied. The composition of the mobile phases had to be shifted to higher contents of organic modifiers -acetonitrile or methanol -in order to elute these hydrophobic compounds from the columns. The question we wanted to answer was: How is the method for testing of reversed phases related to retention, separation efficiency, and peak symmetry of various analytes?

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Section: Introductionmentioning

confidence: 99%

Capillary liquid chromatography as a tool for separation of hydrophobic basic drugs. Relation between tests for column characterization and real analysis

Kafková

Tesařová²,

Suchánková

et al. 2003

J of Separation Science

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“…While this may actually be an advantage in some applications, we have recently obviated this need by adapting PEG-ABS to a solid-supported chromatographic mode (23,24). Although the chromatographic techniques employed probably fit under the category of hydrophobic interaction chromatography (25,26), we prefer to name this separation technique: Aqueous Biphasic Extraction chromatography (ABEC). We believe this acronym is appropriate due to the behavior of our resins and the strong relationship with liquidfliquid PEG-ABS.…”

Section: Introductionmentioning

confidence: 99%

New Technetium-99M Generator Technologies Utilizing Polyethylene Glycol-Based Aqueous Biphasic Systems

Rogers

Bond

Zhang

et al. 1997

Separation Science and Technology

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Two new schemes for TcOJMo0~-separations fiom OH-and MOO: -media using polyethylene glycol (PEG)-based aqueous biphasic systems (ASS) have been developed. The two most important salt solutions in current -Tc-generator technologies, OH-and MOO: , also salt out PEG to form ABS. In liquidfliquid PEG-ABS, pertechnetate can be separated from molybdate with separation factors as high as 10,000. Stripping is accomplished by reduction of the TcO; and back extraction into a salt solution. The strip solution can be the salt of an imaging agent (e.g., Na,HEDPA) and thus may, under the appropriate conditions, be injected directly into the human body. -TcOi can also be concentrated fiom a dilute load solution of ?MOO: in NaOH using an aqueous biphasic extraction chromatographic technique (ABEC). A rinse with KJO, assures that all ?MOO:-is removed from the column and this is confirmed by a rapid drop in ?Mo activity by the fourth free column volume (fcv) of rinse. The T c O i is then eluted with water. This chromatographic separation affords 94% of the V c O ; activity in 5 fcv, with the y spectrum showing less than 2 x IO4 of the original %o activity.

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“…The major applications of these phases are in RPC and HIC permitting very varied separations [20, and there is a wide range of commercial columns of this type on the market; in addition, most evaluation and testing procedures have been designed for these phases, They can be classified into four groups:…”

Section: Approaches For Column Selection and Testingmentioning

confidence: 99%

“…This partly stems from the very different principles and assumptions employed in the individual testing methods and further from the fact that a great variety of test substances, mobile phase compositions and calculation procedures are used. For example, it has been shown [20] that column parameters such as hydrophobicity and hydrophilic@ strongly depend on the nature of the test substance. Unfortunately, inappropriate test substances are sometimes selected when testing columns for peptide and protein analysis, usually small molecules whose behaviour is very different from that of large peptide and protein species.…”

Section: Approaches For Column Selection and Testingmentioning

confidence: 99%

“…The isopotential concept of Bidlingmeyer [29] and the slope-intercept relationships (log of retention factor versus salt molality (HIC) or per cent of organic modifier (RPC)) were recently examined for HIC and RPC [20,23,39], in order to evaluate columns for peptide and protein analysis. The results have been partially confirmed using other testing methods but discrepancies have also been discovered.…”

Section: Approaches For Column Selection and Testingmentioning

confidence: 99%

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Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

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A survey is given of modern stationary phases employed in high performance liquid chromatography (HPLC) analysis of peptides. The physico-chemical properties of peptides and their consequences for the selection and optimization of the separation system are briefly discussed, followed by a summary of the approaches to the selection and characterization of stationary phases. The properties and applicability of various stationary phases are then critically reviewed, including aspects such as size-exclusion, ion-exchange, reversed-phase, hydrophobic-interaction, affinity and chiral systems, as well as some specialized separation techniques. Emphasis is placed on the most recent literature. 0 1997 Elsevier Science B.V.

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Characterization of stationary phases used in reversed-phase and hydrophobic interaction chromatography

Cited by 17 publications

References 22 publications

Capillary liquid chromatography as a tool for separation of hydrophobic basic drugs. Relation between tests for column characterization and real analysis

Capillary liquid chromatography as a tool for separation of hydrophobic basic drugs. Relation between tests for column characterization and real analysis

New Technetium-99M Generator Technologies Utilizing Polyethylene Glycol-Based Aqueous Biphasic Systems

Stationary phases for peptide analysis by high performance liquid chromatography: a review

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