Characterization of stem-like cells in a new astroblastoma cell line

Çoban, Esra Aydemir; Kaşıkcı, Ezgi; Karataş, Ömer Faruk; Suakar, Öznur; Kuşkucu, Ayşegül; Altunbek, Mine; Türe, Uğur; Şahın, Fikrettin; Bayrak, Ömer Faruk

doi:10.1016/j.yexcr.2017.02.030

Cited by 7 publications

(6 citation statements)

References 38 publications

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“…With the advancement of gene identi cation technology, numerous speci c genes have been identi ed in astroblastomas. The most frequent gene alterations detected were MN1 mutation, gain of chromosome arm 20q and chromosome 19, loss of 9q, 10 and X [18][19][20]. These ndings suggested that astroblastomas represent a distinct entity with characteristic cytogenetic features that differ from those of ependymomas and astrocytomas.…”

Section: Discussionmentioning

confidence: 88%

Cerebral astroblastoma with oligodendroglial-like cells：A case report

Gu¹,

Yu²,

Xu³

et al. 2020

Preprint

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Background: Astroblastoma is a rare tumor of the central nervous system, with unclear biological behavior and origin. Its histopathological features have been well established. However, to the best of our knowledge, astroblastoma with oligodendroglial-like cells have not been reported. Case presentation: Herein, we reported a case of astroblastoma with atypical pathological features. A 15-year-old girl presented with nausea, vomiting, headache and visual disturbance. Magnetic resonance imaging (MRI) revealed a large neoplasm in the left temporal lobe. Histologically, the tumor showed solid and pseudopapillary structure. The tumor cells were radially arranged around blood vessels, with a single, prominent process, and as astroblastic pseudorosettes. More importantly, typical oligodendroglial-like cells were observed. In addition to membrane staining for EMA, immunohistochemical staining also showed that the tumor cells were positive for GFAP and Vimentin. The oligodendroglial-like cells were positive for GFAP, Vimentin, and Olig-2. The Ki-67 labeling index was about 4%. Sequencing for IDH1 codon 132 and IDH2 codon 172 gene mutations showed negative results. Furthermore, fluorescent analysis revealed neither 1p nor 19q deletion in the lesion. Based on these findings, the tumor was finally diagnosed as astroblastoma. Conclusions: Herein, we reported an extremely rare case of astroblastoma, which was morphologically characterized by the appearance of oligodendroglial-like cells.

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Section: Discussionmentioning

confidence: 88%

Cerebral astroblastoma with oligodendroglial-like cells：A case report

Gu¹,

Yu²,

Xu³

et al. 2020

Preprint

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“…But as the technology of gene identification matures, more and more specific genes are identified in astroblastoma. The most frequent genes alterations detected were meningioma 1 mutation, gains of chromosome arm 20q, and chromosome 19, losses on 9q, 10, and X [18–20] . These suggested that astroblastomas represent a distinct entity with characteristic cytogenetic features that differ from those of ependymomas and astrocytomas.…”

Section: Discussionmentioning

confidence: 94%

“…The most frequent genes alterations detected were meningioma 1 mutation, gains of chromosome arm 20q, and chromosome 19, losses on 9q, 10, and X. [ 18 – 20 ] These suggested that astroblastomas represent a distinct entity with characteristic cytogenetic features that differ from those of ependymomas and astrocytomas. Because astroblastomas do not have gene detection for large sample cases, there is no unified gene mutation spectrum.…”

Section: Discussionmentioning

confidence: 99%

Cerebral astroblastoma with oligodendroglial-like cells

Wang

2021

Medicine

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Rationale: Astroblastoma is a rare tumor of the central nervous system with uncertain biological behavior and origin. Its histopathological features have been well established, while, to our knowledge, astroblastoma with oligodendroglial-like cells have not been reported.Patient concerns: A 15-year-old girl presented with nausea, vomiting, headache, and visual disturbance.Diagnosis: Magnetic resonance imaging revealed a large neoplasm in the left temporal. Histologically, the tumor showed solid and pseudopapillary structure. Immunohistochemical staining showed that the tumor cells were positive for glial fibrillary acidic protein and vimentin. The oligodendroglial-like cells were positive for glial fibrillary acidic protein, vimentin, and oligodendrocyte transcription factor 2. The antigen KI67 labeling index was about 4%. Sequencing for isocitrate dehydrogenase (IDH) 1 codon 132 and IDH2 codon 172 gene mutations showed negative results. Furthermore, fluorescent analysis revealed neither 1p nor 19q deletion in the lesion. Based on these findings, the girl was finally diagnosed as astroblastoma.Interventions: A craniotomy with total excision of the tumor was performed.Outcomes: The follow-up time was 1 year, no evidence of disease recurrence was found in magnetic resonance imaging.Lessons: Cerebral astroblastoma with oligodendroglial-like cells is a clinically rare tumor of central nervous system. Clear distinction and diagnosis are critical. Abbreviations: EMA = epithelial membrane antigen, FISH = fluorescent in situ hybridization, GFAP = glial fibrillary acidic protein, IDH = isocitrate dehydrogenase, NeuN = neuronal nuclear antigen, Olig-2 = oligodendrocyte transcription factor 2, P53 = tumor protein 53, Syn = synaptophysin.

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“…The effect of exosome treatment on cell cycle was determined by the protocol described previously [17]. Shortly, NP cells -treated with exosomes-were harvested and suspended with ice-cold 70% ethanol for at least 2 hours prior to the analysis.…”

Section: Cell Cycle Assaymentioning

confidence: 99%

Investigating the Effects of Chordoma Cell-Derived Exosomes on the Tumorigenicity of Nucleus Pulposus Cells

Aydemir

Yılmaz

Bayrak

et al. 2023

J Neurol Surg B Skull Base

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Objective Interaction of tumor cells with the surrounding environment is essential for tumor growth and progression that eventually leads to metastasis. Growing evidence shows that extracellular vesicles (EVs) also known as exosomes play a crucial role in signalling between the tumor and its microenvironment. Tumor-derived exosomes have generally pro tumorigenic effects such as metastasis, hypoxia, angiogenesis and epithelial-mesenchymal transition (EMT). Methods In this study, exosomes were isolated from a chordoma cell line, MUG-Chor1, and characterized subsequently. The number of exosomes were determined and introduced into the healthy nucleus pulposus (NP) cells for 140 days. The pro-tumorigenic effects of a chordoma cell line-derived exosomes that initiate the tumorigenesis on NP cells were investigated. The impact of tumor-derived exosomes on various cellular events including cell cycle, migration, proliferation, apoptosis, and viability have been studied by treating NP cells with chordoma cell-line derived exosomes cells. Results Upon treatment with exosomes, the NP cells not only gained a chordoma-like morphology but also molecular characteristics such as alterations in the levels of certain gene expressions. The migratory and angiogenic capabilities of NP cells increased after treated with chordoma-derived exosomes. Conclusion Based on our findings, we can conclude that exosomes carry information from tumor cells and may exert tumorigenic effects on non-tumorous cells.

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Characterization of stem-like cells in a new astroblastoma cell line

Cited by 7 publications

References 38 publications

Cerebral astroblastoma with oligodendroglial-like cells：A case report

Cerebral astroblastoma with oligodendroglial-like cells：A case report

Cerebral astroblastoma with oligodendroglial-like cells

Investigating the Effects of Chordoma Cell-Derived Exosomes on the Tumorigenicity of Nucleus Pulposus Cells

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