Characterization of Sterol Demethylation Inhibitor-Resistant Isolates of <i>Fusarium asiaticum</i> and <i>F. graminearum</i> Collected from Wheat in China

Yin, Yanni; Liu, Xiaoyu; B, Li; Ma, Zhonghua

doi:10.1094/phyto-99-5-0487

Cited by 157 publications

(124 citation statements)

References 27 publications

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“…Contrastingly, Klix et al (2007), assessed fungicide sensitivity of F. graminearum isolates, based on the ascosporic germination rate, and observed a larger decline in sensitivity over time (1987 to 2004) for tebuconazole, followed by metconazole and prothioconazole. In China, Yin et al, (2009) assessed sensitivity of 40 F. graminearum isolates to tebuconazole and found a EC 50 mean of 0.248 mg/L (0.021-1.152 mg/L), which is much higher than those found in our study to both fungicides tested. However, in Japan, Tateishi et al (2010) studied 101 isolates representing F. graminearum and F. asiaticum populations and found that 80% of isolates showed EC 50 towards metconazole of less than 0.1mg/L, a level of sensitivity more similar to that found in the present study.…”

contrasting

confidence: 73%

“…In Europe and Asia, the increasing usage of triazoles in FHB management is suspected to have led to selection of less sensitive isolates of F. graminearum (Klix et al, 2007;Yin et al, 2009;Becher et al, 2010). Fungal resistance to triazoles is associated with mutations in the 14-α-demethylase (CYP51) gene and overexpression of the ATPbinding cassette transporters (Ma and Michailides, 2005;Liu et al, 2010).…”

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confidence: 99%

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Sensitivity of Fusarium graminearum causing head blight of wheat in Brazil to tebuconazole and metconazole fungicides

Spolti

Jorge

Ponte

2012

Trop. plant pathol.

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Fusarium head blight (FHB) of wheat, is caused by fungal populations belonging to the Fusarium graminearum species complex. Although triazole fungicides are widely used in FHB management, information about the sensitivity of F. graminearum populations to such fungicides is scarce, particularly in Brazil. This work aimed at determining the sensitivity of pathogenic populations of F. graminearum to metconazole and tebuconazole. Fungal isolates were obtained from diseased wheat heads collected in commercial fields at the northern region of Rio Grande do Sul state, Brazil, along the 2009 and 2010 growing seasons. Fifty isolates obtained from eleven locations were grown in media amended with increasing fungicide dosages and the effective concentration reducing mycelial growth to 50% (EC 50 ) was determined. The median of the EC 50 values was 0.001 mg/L (<0.001-0.152 mg/L) and 0.037 mg/L (<0.001-0.324), for tebuconazole and metconazole, respectively. Cross-resistance was detected based on significance of correlation analysis (r s = 0.46; P < 0.0001) between the log-transformed EC 50 of the two triazoles. The mean EC 50 for a group of ten isolates classified as "more sensitive" differed statistically from the ten isolates representing the "less sensitive" group. Nevertheless, these groups did not differ in terms of mycelial growth on PDA in the absence of fungicides. It was concluded that pathogenic populations of F. graminearum are sensitive to the triazoles at various levels due to the intrinsic characteristics of the isolates or to selection pressure by the increasing fungicide use.

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confidence: 73%

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confidence: 99%

Sensitivity of Fusarium graminearum causing head blight of wheat in Brazil to tebuconazole and metconazole fungicides

Spolti

Jorge

Ponte

2012

Trop. plant pathol.

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“…Para o manejo da giberela e das micotoxinas nos grãos, é recomendado o uso de cultivares com maior grau de resistência, aplicação de fungicidas e o ajuste dos mecanismos da colheita mecânica para a eliminação de grãos giberelados (McMULLEN et al, 2012). Fungicidas do grupo dos triazóis, que possuem ação fungistática sobre F. graminearum sensu lato, têm sido indicados para uso durante o fl orescimento pleno (YIN et al, 2009). Considerando que grãos giberelados são menores e mais leves do que grãos sadios, especialmente aqueles infectados no início do fl orescimento, o ajuste dos mecanismos da colheita mecânica pode facilitar a eliminação de grãos giberelados e, consequentemente, reduzir os níveis de micotoxinas nos lotes de grãos colhidos (SALGADO et al, 2011).…”

Section: Introductionunclassified

Agressividade diferencial de espécies do complexo Fusarium graminearum em interação com o fungicida tebuconazole na redução do rendimento de trigo

Spolti

Ponte

2013

Cienc. Rural

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“…A few reports 30) have investigated the sensitivity to DMIs and FHB pathogen species but no report has examined the relationship between DMI sensitivity and trichothecene chemotype. Nor has any paper investigated the relationship between the efficacy of DMI and the sensitivity of FHB causal fungi to DMI.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Japanese Fusarium graminearum species complex isolates to metconazole

et al. 2010

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The sensitivity of the Fusarium graminearum species complex, the causal fungus of Fusarium head blight, to metconazole was measured. The minimum inhibitory concentrations (MIC) of 101 isolates ranged from 0.20 to 6.25 mg/l with a single peak at 1.56 mg/l. The effective concentration for 50% growth inhibition (EC 50 ) was Ͻ0.1 mg/l in about 80% of isolates, and no isolate was significantly less sensitive to metconazole among this group. Among the F. graminearum species complex, F. asiaticum and F. graminearum s. str. were identified by PCR-RFLP. The trichothecene chemotypes were determined as 3ADON, 15ADON, or NIV by multiplex PCR. Although species-specific geographical distributions and mycotoxin production characteristics were found, the MIC values to metconazole of both species were distributed within a similar range, and no significant difference in sensitivity was observed between the species or trichothecene chemotypes. Next, CYP51 genes from isolates with different sensitivities to metconazole were amplified by PCR, and their sequences were compared. As a result, it was suggested that the differences in sensitivity to metconazole between the isolates were not due to the substitution of amino acids in CYP51, the target enzyme of DMI. Then, macrospores from isolates with various sensitivities to metconazole were sprayed onto wheat ears, and the efficacy of metconazole was examined. Metconazole showed high control activity against every isolate.

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Characterization of Sterol Demethylation Inhibitor-Resistant Isolates of Fusarium asiaticum and F. graminearum Collected from Wheat in China

Cited by 157 publications

References 27 publications

Sensitivity of Fusarium graminearum causing head blight of wheat in Brazil to tebuconazole and metconazole fungicides

Sensitivity of Fusarium graminearum causing head blight of wheat in Brazil to tebuconazole and metconazole fungicides

Agressividade diferencial de espécies do complexo Fusarium graminearum em interação com o fungicida tebuconazole na redução do rendimento de trigo

Sensitivity of Japanese Fusarium graminearum species complex isolates to metconazole

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