Characterization of streptococcus-like bacteria from diseased Oreochromis niloticus farmed on Lake Kariba in Zambia

Bwalya, Patricia; Simukoko, Chalumba Kachusi; Hang’ombe, Bernard M.; Støre, Sofie C; Peder, Store; Gamil, Amr Ahmed Abdelrahim; Evensen, Øystein; Mutoloki, Stephen

doi:10.1016/j.aquaculture.2020.735185

Cited by 13 publications

(39 citation statements)

References 22 publications

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“…The carbohydrate fermentation test results gave positive results because the changed from red to yellow colour due to phenol red indicator that changed the colour in the acidic pH, this is in agreement with Belhadj et al (2014). β hemolytic Streptococcus could ferment several sugars and gave positive results for Glucose, Maltose, Sucrose, Lactose, Arabinose, Galactose, Sorbitol, D-Mannose while could not fermentation (negative results) of Mannitol, Inositol, this result was an agreement with Bwalya et al (2020), Lucia et al (2017, Adikesavalu et al (2017), Hardi et al (2011), Pourgholam et al (2011.…”

Section: Discussionsupporting

confidence: 78%

Isolation and Identification of GBS Bacteria From Mastitis by Camp Test and Lancefield’s Serological Grouping

Alkader¹,

Hyyawi²

2021

PLANT ARCHIVES

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Mastitis is an udder tissue inflammation which has infected various species of animals. It happens through several types of pathogenic bacteria, particularly Streptococcus agalactiae. GBS is a leading cause of cow mastitis. In our sample, 9.52% of Streptococcus agalactiae were isolated which were collected from bovine mastic milk and identified by biochemical tests such as catalase, oxidase, Production of indole, fermentation of sugar, an examination of antibiotic sensitivity, CAMP test and group kits of Lancefield. The results showed that all Streptococcus agalactiae isolate was diagnosed by CAMP test by the appearance of the arrowhead in blood agar and by the appearance of visible agglutination on a card in the serological grouping kit of Lancefield.

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Section: Discussionsupporting

confidence: 78%

Isolation and Identification of GBS Bacteria From Mastitis by Camp Test and Lancefield’s Serological Grouping

Alkader¹,

Hyyawi²

2021

PLANT ARCHIVES

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“…The experiment to determine the 50% endpoint (LD 50 ) is described elsewhere (Bwalya, Simukoko, et al, 2020). Briefly, 100 Nile tilapia ( Oreochromis niloticus ) were divided into 7 groups of 10 fish each.…”

Section: Methodsmentioning

confidence: 99%

“…Lactococcus garvieae previously isolated from diseased Nile tilapia on Lake Kariba in Siavonga district (Bwalya, Simukoko, et al, 2020) was used. The isolate had been stored in Brain Heart Infusion Broth (BHIB [Unipath, England]) with 20% glycerol at −20°C until used.…”

Section: Preparation Of Lactococcus Garvieae For Challengementioning

confidence: 99%

Lactococcus garvieae isolated from Lake Kariba (Zambia) has low invasive potential in Nile tilapia (Oreochromis niloticus)

Bwalya

Hang’ombe

Evensen

et al. 2021

Journal of Fish Diseases

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The pathogenesis of Lactococcus garvieae (L. garvieae) was assessed in Nile tilapia (Oreochromis niloticus) following administration by two different routes of infection (intraperitoneal versus immersion), using 180 fish divided into three groups. The first group of fish was injected intraperitoneally (IP) with 3 × 105 colony‐forming units (cfu) of L. garvieae; the second group was infected by immersion (IMM) into water containing 9.6 × 105 cfu/ml L. garvieae, and in group 3 (Control), the fish were injected IP with sterile normal saline. Mortalities were recorded daily, and on 3, 5, 7, and 13 days post‐infection (dpi), liver, kidney, spleen, brain and eyes were sampled. The level of infection between groups was assessed by number of mortalities that occurred, pathology/histopathology of internal organs, bacterial re‐isolation and presence of bacteria in situ determined using immunohistochemistry. A significant difference (p < .0001) was observed between L. garvieae re‐isolation from tilapia following administration by IP injection and IMM. Similarly, more clinical signs and mortalities (p < .001) were observed in the IP group compared to the IMM group where no mortalities were observed. These findings suggest that L. garvieae has a low invasive potential in Nile tilapia with intact skin/external barriers and highlights the importance of maintaining fish without cuts or abrasions under field conditions.

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“…As with intensive fish farming elsewhere that is affected by fish diseases [3], the increase in the fish production on Lake Kariba soon faced the same problem. Lactococcosis outbreaks caused by Lactococcus garvieae (L. garvieae) have been experienced since early this decade [4].…”

Section: Introductionmentioning

confidence: 99%

A whole-cell Lactococcus garvieae autovaccine protects Nile tilapia against infection

et al. 2020

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The autovaccine was produced in-house using a bacterial isolate from a diseased fish from the target farm. Three groups of 150 fish each were injected with either 1) an oil-adjuvanted, inactivated whole cell autovaccine, 2) adjuvant only or 3) PBS (negative control). Approximately 660 degree days post vaccination, the fish were challenged with 9x10 5 cfu bacteria/ fish by intraperitoneal injection and monitored for a further 28 days. Protection against infections was measured by lack of/reduced bacterial loads both by bacterial re-isolation and immunohistochemistry as well as absence of clinical signs/pathology. Significantly less L. garvieae (p<0.03) was re-isolated from either the adjuvant only or control groups compared to the vaccinated group. Furthermore, a significantly high amount (p<0.001) of anti-L. garvieae specific antibodies were observed in the vaccinated group compared to the adjuvant only or control groups at time of challenge. This coincided with protection against infection measured by absence/reduced L. garvieae re-isolation from internal organs, reduced clinical signs and lack of pathology in this group. In the adjuvant only and control groups, bacteria were re-isolated from the kidney, liver, spleen, brain and eyes during the first 14 days. The findings suggest that oil-based vaccines can protect tilapia against L. garvieae infection through an antibody mediated response.

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Characterization of streptococcus-like bacteria from diseased Oreochromis niloticus farmed on Lake Kariba in Zambia

Cited by 13 publications

References 22 publications

Isolation and Identification of GBS Bacteria From Mastitis by Camp Test and Lancefield’s Serological Grouping

Isolation and Identification of GBS Bacteria From Mastitis by Camp Test and Lancefield’s Serological Grouping

Lactococcus garvieae isolated from Lake Kariba (Zambia) has low invasive potential in Nile tilapia (Oreochromis niloticus)

A whole-cell Lactococcus garvieae autovaccine protects Nile tilapia against infection

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