Characterization of substrate specificity and inhibitory mechanism of bile salt hydrolase from Lactobacillus reuteri CRL 1098 using molecular docking analysis

Ledesma, Ana Estela; Taranto, María Pía; Bustos, Ana Yanina

doi:10.1007/s10529-021-03097-y

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…The result of molecular docking matched the distance range for many enzyme-substrate interactions. The binding energy was consistent with previous reports that suggested the possibility of enzyme-substrate interactions [35,36]. Compared with the Figure 7.…”

Section: Discussionsupporting

confidence: 91%

Insight into the High-Efficiency Benzo(a)pyrene Degradation Ability of Pseudomonas benzopyrenica BaP3 and Its Application in the Complete Bioremediation of Benzo(a)pyrene

Dong,

Wu,

Rao

et al. 2023

IJMS

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Polycyclic aromatic hydrocarbons (PAHs) are common carcinogens. Benzo(a)pyrene is one of the most difficult high-molecular-weight (HMW) PAHs to remove. Biodegradation has become an ideal method to eliminate PAH pollutants from the environment. The existing research is mostly limited to low-molecular-weight PAHs; there is little understanding of HMW PAHs, particularly benzo(a)pyrene. Research into the biodegradation of HMW PAHs contributes to the development of microbial metabolic mechanisms and also provides new systems for environmental treatments. Pseudomonas benzopyrenica BaP3 is a highly efficient benzo(a)pyrene-degrading strain that is isolated from soil samples, but its mechanism of degradation remains unknown. In this study, we aimed to clarify the high degradation efficiency mechanism of BaP3. The genes encoding Rhd1 and Rhd2 in strain BaP3 were characterized, and the results revealed that rhd1 was the critical factor for high degradation efficiency. Molecular docking and enzyme activity determinations confirmed this conclusion. A recombinant strain that could completely mineralize benzo(a)pyrene was also proposed for the first time. We explained the mechanism of the high-efficiency benzo(a)pyrene degradation ability of BaP3 to improve understanding of the degradation mechanism of highly toxic PAHs and to provide new solutions to practical applications via synthetic biology.

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Section: Discussionsupporting

confidence: 91%

Insight into the High-Efficiency Benzo(a)pyrene Degradation Ability of Pseudomonas benzopyrenica BaP3 and Its Application in the Complete Bioremediation of Benzo(a)pyrene

Dong,

Wu,

Rao

et al. 2023

IJMS

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“…BSH enzymes are represented across most bacterial phyla. Regarding the commensal gut microbiota, BSH activity is found in Gram-positive bacteria: Lactobacillus , Bifidobacterium [ 3 , 17 , 18 ], Clostridium spp., and Enterococcus [ 19 , 20 ]. However, they are also detected in some commensal Gram-negative strains, such as the Bacteroides spp [ 3 , 12 , 21 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

Farnesoid X Receptor, Bile Acid Metabolism, and Gut Microbiota

et al. 2022

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Obesity, type 2 diabetes, and non-alcoholic fatty liver disease (NAFLD) are characterized by the concepts of lipo- and glucotoxicity. NAFLD is characterized by the accumulation of different lipidic species within the hepatocytes. Bile acids (BA), derived from cholesterol, and conjugated and stored in the gallbladder, help the absorption/processing of lipids, and modulate host inflammatory responses and gut microbiota (GM) composition. The latter is the new “actor” that links the GI tract and liver in NAFLD pathogenesis. In fact, the discovery and mechanistic characterization of hepatic and intestinal farnesoid X receptor (FXR) shed new light on the gut–liver axis. We conducted a search on the main medical databases for original articles, reviews, meta-analyses of randomized clinical trials, and case series using the following keywords, their acronyms, and their associations: farnesoid X receptor, bile acids metabolism, gut microbiota, dysbiosis, and liver steatosis. Findings on the synthesis, metabolism, and conjugation processes of BAs, and their action on FXR, change the understanding of NAFLD physiopathology. In detail, BAs act as ligands to several FXRs with GM modulation. On the other hand, the BAs pool is modulated by GM, thus, regulating FXRs functioning in the frame of liver fat deposition and fibrosis development. In conclusion, BAs passed from their role of simple lipid absorption and metabolism agents to messengers between the gut and liver, modulated by GM.

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“…Based on previous work, this study, which comprehensively assessed BSH‐encoding gut bacteria through multivariate analysis and data mining of extensive metagenome data, discovered crucial BSH‐encoding lactobacilli species. Compared with other BSH‐encoding genera, lactobacilli was the most reported (Supporting Information: Figure S11 , Table S12 ), including L. gasseri [ 22 ], L. reuteri [ 33 ], L. salivarius [ 34 ], and so on. A previous study reported about 39 BSH‐encoding lactobacilli species, but including some species that are not indigenous to the gut, such as L. agilis and L. vaginalis , which are typically found in the saliva and the vagina, respectively [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Taxonomic identification of bile salt hydrolase‐encoding lactobacilli: Modulation of the enterohepatic bile acid profile

Song

Feng

Zhou

et al. 2023

iMeta

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Bile salt hydrolases (BSHs) are enzymes that are essential for the enterohepatic metabolism of bile acids (BAs). BSHs catalyze the production of unconjugated BAs and regulate the homeostasis of BA pool. This study identified Lactobacillus as a crucial BSH‐encoding genus, and 16 main species were obtained using metagenomic data from publicly available human gut microbiome databases. Then, the 16 species of lactobacilli were classified into four typical categories by BSH phylotypes, including five species encoding BSH‐T0, six species encoding BSH‐T2, four species encoding BSH‐T3, and Ligilactobacillus salivarius encoding both BSH‐T0 and BSH‐T3. The lactobacilli with the highest in vitro deconjugation activities against seven conjugated BAs were the BSH‐T3‐encoding strains. Furthermore, in vivo studies in mice administered four representative lactobacilli strains encoding different BSH phylotypes showed that treatment with BSH‐T3‐encoding Limosilactobacillus reuteri altered the structure of the gut microbiome and metabolome and significantly increased the levels of unconjugated BAs and total BA excretion. Our findings facilitated the taxonomic identification of crucial BSH‐encoding lactobacilli in human gut microbiota and shed light on their contributions toward modulation of the enterohepatic circulation of BAs, which will contribute to future therapeutic applications of BSH‐encoding probiotics to improve human health.

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Characterization of substrate specificity and inhibitory mechanism of bile salt hydrolase from Lactobacillus reuteri CRL 1098 using molecular docking analysis

Cited by 4 publications

References 16 publications

Insight into the High-Efficiency Benzo(a)pyrene Degradation Ability of Pseudomonas benzopyrenica BaP3 and Its Application in the Complete Bioremediation of Benzo(a)pyrene

Insight into the High-Efficiency Benzo(a)pyrene Degradation Ability of Pseudomonas benzopyrenica BaP3 and Its Application in the Complete Bioremediation of Benzo(a)pyrene

Farnesoid X Receptor, Bile Acid Metabolism, and Gut Microbiota

Taxonomic identification of bile salt hydrolase‐encoding lactobacilli: Modulation of the enterohepatic bile acid profile

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