Characterization of sulfate and phosphate containing plasma polymer surfaces

Siow, Kim Shyong; Britcher, Leanne; Kumar, Saravana; Griesser, Hans J.

doi:10.1109/iconn.2006.340612

Cited by 4 publications

(6 citation statements)

References 16 publications

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“…The spectra of the Thermanox substrate (Figure 2C) can be easily visually distinguished from those from CSMs (Figures 2D, 2E & 2F) and adsorbed proteins (Figures 2A & 2B). Thermanox is understood to be a custom poly(ethylene terephthalate) (PET) resin subsequently functionalized to contain nitrogen based groups to improve cell adhesion [60]. Many prominent peaks are known fragments of the PET backbone, including the PET-repeat unit (m/z 193) and the benzyol fragment C 7 H 5 O + (m/z 104), whilst others, not seen in the spectra of pure PET previously reported [61] can be identified as nitrogen containing organic secondary ions, presumably resulting from proprietary modifications to PET, including negative ions m/z 26 and m/z 42.…”

Section: Resultsmentioning

confidence: 99%

Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry

et al. 2015

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Cell-secreted matrices (CSMs), where extracellular matrix (ECM) deposited by monolayer cell cultures are decellularized, have been increasingly used to produce surfaces that may be reseeded with cells. Such surfaces are useful to help us understand cell-ECM interactions in a microenvironment closer to the in vivo situation than synthetic substrates with adsorbed proteins. We describe the production of CSMs from mouse primary osteoblasts (mPObs) exposed to cytokine challenge during matrix secretion, mimicking in vivo inflammatory environments. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) data revealed that CSMs with cytokine challenge at day 7 or day 12 of culture can be chemically distinguished from one another and from untreated CSM using multivariate analysis. Comparison of the differences with reference spectra from adsorbed protein mixtures points towards cytokine challenge resulting in a decrease in collagen content. This is supported by immunocytochemical and histological staining, demonstrating a 44% loss of collagen mass and a 32% loss in collagen I coverage. CSM surfaces demonstrate greater cell adhesion than adsorbed ECM proteins. When mPObs were reseeded onto cytokine-challenged CSMs they exhibited reduced adhesion and elongated morphology compared to untreated CSMs. Such changes may direct subsequent cell fate and function and provide insights into pathological responses at sites of inflammation.

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Section: Resultsmentioning

confidence: 99%

Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry

et al. 2015

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“…33 Peaks observed at around 2958, 2935, 2873 and 1703 cm −1 were attributed to the C-H stretching vibrations. 22,34,35 Peaks observed between 2700 and 2100 cm −1 were attributed to the acidic phosphorus acid O-H stretching vibrations. P-H stretching vibrations of basic phosphine groups were observed at 2028 and 807 cm −1 .…”

Section: Ftir-atr Spectroscopymentioning

confidence: 99%

“…P=O stretching vibrations were observed around 1380 and 1234 cm −1 , while the P-O-C stretching vibration was observed at 1054 cm −1 . 22,34,35 Although the phosphorus acid O-H and P-H groups were not present in the DP monomer, they were present on the surface after plasma functionalization, which is probably due to the random breaking down and reformation of new bonds during the plasma process. FTIR data also indicate that after plasma modification, both acidic and basic groups (proton donor and acceptor groups) were present on the surface.…”

Section: Ftir-atr Spectroscopymentioning

confidence: 99%

“…The percentage of these components can be determined using CF procedures with the assumption that the effective absorptivities of all components are equal. 22,34,39,40 In Figure 3, peaks observed between 1695 and 1670 and 1625 and 1610 cm −1 are assigned to the intermolecular b-structure; between 1690 and 1680 and 1640 and 1630 cm −1 , to the intramolecular b-structure; between 1666 and 1659 cm −1 , to the 'three-turn'…”

Section: Protein Adhesion and Conformational Changesmentioning

confidence: 99%

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In vitro and in vivo bacterial antifouling properties of phosphite plasma-treated silicone

Akdoğan

Demirbilek

Şen

et al. 2019

Surface Innovations

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In order to improve their bacterial antifouling property, silicone surfaces were functionalized through the plasma polymerization (PP) technique using diethyl phosphite as the precursor. The functionalized surfaces were characterized using contact angle measurements, contact angle titration, Fourier transform infrared-attenuated total reflection spectroscopy and in vitro cytotoxicity assay. The amount of non-specific protein adsorption and the conformational changes of surface-adsorbed proteins were investigated. Antifouling properties of the surfaces were evaluated in vitro and in vivo. PP functionalization generated a hydrophilic and amphoteric surface with a very good protein and bacterial antifouling property and caused less conformational changes on the secondary structure of surface-adsorbed proteins. In in vivo conditions, no slime layer was formed around bacteria that adhered on the PPfunctionalized surface. It is concluded that the amphoteric nature of the PP-functionalized surface is the reason for the good antifouling property.

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“…4,11 Plasma sources represent a quick and clean method for the surface modification of polymers. 12,13 In particular, atmospheric plasma treatment (APT) has many advantages in terms of time savings, operating costs, and maintenance compared to reduced pressure systems. 14 APT has the benefit of being performed at a low temperature, which prevents overheating and damage to the materials being treated.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric plasma treatment to improve the surface properties of P3HB coating. A study of the influence of substrate roughness

Martinez,

Vázquez‐Vélez,

Galván‐Hernández

et al. 2023

J of Applied Polymer Sci

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Polyhydroxybutyrate (P3HB) is a biocompatible polymer. However, its hydrophobic surface can disrupt cell adhesion, which is essential in implanted biomaterials. Atmospheric plasma treatment is an environmentally friendly method that improves surface properties. This study analyzed P3HB coatings obtained on stainless steel by various mechanical interlocking and their plasma process. The surface free energy and the degree of crystallinity of coatings increased with the plasma treatment. Atomic force microscopy analysis showed that roughness improved, and adhesion energy increased by a factor of three after plasma treatment by inserting polar groups analyzed with Raman spectroscopy. Based on this, a mechanism of chemical functionalization by plasma on the surface is proposed. The results depend on the initial properties given by the mechanical interlocking at the metal‐polymer interface.

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Characterization of sulfate and phosphate containing plasma polymer surfaces

Cited by 4 publications

References 16 publications

Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry

Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry

In vitro and in vivo bacterial antifouling properties of phosphite plasma-treated silicone

Atmospheric plasma treatment to improve the surface properties of P3HB coating. A study of the influence of substrate roughness

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