Characterization of sunlight-grown transgenic rice plants expressing Arabidopsis phytochrome A

Kong, Sam‐Geun; Lee, Dae-Seok; Kwak, Su-Nam; Kim, Ju-Kon; Sohn, Jae-Keun; Kim, In-Soo

doi:10.1023/b:molb.0000037993.79486.7b

Cited by 28 publications

(29 citation statements)

References 35 publications

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“…For example, while expression of rice PHYA in tobacco variety Xanthi has been reported to result in shorter stems and dark green leaves (Keller et al 1989;McCormac et al 1991McCormac et al , 1993Nagatani et al 1991), a comparable study in which rice PHYA gene was expressed in the tobacco variety SR1 showed no morphological phenotype associated with transgene expression (Kay et al 1989). Similar background differences may account for the variation seen between the japonica rice lines characterized by Kong et al (2004) and the indica rice lines characterized in this study. In contrast to reduction in tiller number per hill in japonica transgenic lines (Kong et al 2004), the indica transgenic lines had relatively higher number of tillers and increased grain weight per plant.…”

Section: Discussionsupporting

confidence: 63%

“…Additionally, there may be differences in the stability of Arabidopsis PHYA and oat PHYA when expressed in rice. Both these effects may contribute to the differences observed between the study of Clough et al (1995) and that of Kong et al (2004) and the results reported here. In general, there was no apparent relationship observed between PHYA level and phenotypic consequences in the various transgenic rice lines.…”

Section: Discussionmentioning

confidence: 55%

“…However, although transgenic lines expressed three to four times more spectrally active phytochrome than that of non-transgenic lines, there were no morphological differences between transgenic and nontransgenic mature plants. In a separate study Kong et al (2004) expressed Arabidopsis PHYA in a different japonica rice variety (Nakdong) under the control of the rice rbcS promoter. In this instance the level of accumulation of the heterologous PHYA was higher than in the study of Clough et al (1995) and clear morphological effects were seen.…”

Section: Discussionmentioning

confidence: 99%

“…The construction of binary plasmid, pSBR 3.7 containing a full-length Arabidopsis thaliana L. (ecotype Columbia) PHYA cDNA (Sharrock and Quail 1989) in the pSB11 vector (Komari et al 1996) is described in Kong et al (2004). The components of the plasmid within the T-DNA region, and the selected restriction enzyme sites are shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Constitutive overexpression of an oat PHYA gene in rice (Oryza sativa L. Gulfmont) and wheat (Triticum aestivum L. Florida) failed to result in dramatic phenotypic effects (Clough et al 1995;Shlumukov et al 2001). However, tissue-specific overexpression of Arabidopsis PHYA in japonica rice (Oryza sativa L. Nakdong) resulted in reduced plant height, larger grain size, higher chlorophyll content, and lower tillering ability of sun-light grown rice plants (Kong et al 2004). Thus, the emerging picture from the studies on overexpression of a monocot or a dicot PHYA in cereal plants is one of a complex interplay between photoreceptors and downstream responses (Sawers et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield

Garg

Sawers

Wang

et al. 2005

Planta

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The phytochromes are a family of red/far-red light absorbing photoreceptors that control plant developmental and metabolic processes in response to changes in the light environment. We report here the overexpression of Arabidopsis thaliana PHYTOCHROME A (PHYA) gene in a commercially important indica rice variety (Oryza sativa L. Pusa Basmati-1). The expression of the transgene was driven by the light-regulated and tissue-specific rice rbcS promoter. Several independent homozygous sixth generation (T(5)) transgenic lines were characterized and shown to accumulate relatively high levels of PHYA protein in the light. Under both far-red and red light, PHYA-overexpressing lines showed inhibition of the coleoptile extension in comparison to non-transgenic seedlings. Furthermore, compared with non-transgenic rice plants, mature transgenic plants showed significant reduction in plant height, internode length and internode diameter (including differences in cell size and number), and produced an increased number of panicles per plant. Under greenhouse conditions, rice grain yield was 6-21% higher in three PHYA-overexpressing lines than in non-transgenic plants. These results demonstrate the potential of manipulating light signal-transduction pathways to minimize the problems of lodging in basmati/aromatic rice and to enhance grain productivity.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield

Garg

Sawers

Wang

et al. 2005

Planta

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Rice

Emani

Jiang

Hall

et al. 2008

Compendium of Transgenic Crop Plants

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Rice serves as the principal source of nourishment for over half of the global population. The year 2004 was declared as the international year of rice by the Food and Agricultural Organization, to heighten awareness of this crop in combating global poverty and malnutrition. The present review is a comprehensive account of this model monocot crop tracing its history, botanical descriptions, and the research work in breeding and biotechnology. An exhaustive illustrative account of all the important milestones in plant breeding, particle bombardment, Agrobacterium ‐mediated transformation, and genomics has been provided, with a focus on other important aspects such as transgene silencing, biosafety regulations of transgenic plants, and the global consequences of rice biotechnology research.

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Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B

Figueroa‐Balderas

Chi-Ham

et al. 2020

Plant Direct

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractThe constitutively active missense allele of Arabidopsis phytochrome B, AtPHYB Y276H or AtYHB, encodes a polypeptide that adopts a light-insensitive, physiologically active conformation capable of sustaining photomorphogenesis in darkness. Here, we show that the orthologous OsYHB allele of rice phytochrome B (OsPHYB Y283H ) also encodes a dominant "constitutively active" photoreceptor through comparative phenotypic analyses of AtYHB and OsYHB transgenic lines of four eudicot species, Arabidopsis thaliana, Nicotiana tabacum (tobacco), Nicotiana sylvestris and Solanum lycopersicum cv. MicroTom (tomato), and of two monocot species, Oryza sativa ssp. japonica and Brachypodium distachyon. Reciprocal transformation experiments show that the gainof-function constitutive photomorphogenic (cop) phenotypes by YHB expression are stronger in host plants within the same class than across classes. Our studies also reveal additional YHB-dependent traits in adult plants, which include extreme shade tolerance, both early and late flowering behaviors, delayed leaf senescence, reduced tillering, and even viviparous seed germination. However, the strength of these gainof-function phenotypes depends on the specific combination of YHB allele and species/cultivar transformed. Flowering and tillering of OsYHB-and OsPHYB-expressing lines of rice Nipponbare and Kitaake cultivars were compared, also revealing differences in YHB/PHYB allele versus genotype interaction on the phenotypic behavior of the two rice cultivars. In view of recent evidence that the regulatory activity of AtYHB is not only light insensitive but also temperature insensitive, selective YHB expression is expected to yield improved agronomic performance of both dicot and monocot crop plant species not possible with wild-type PHYB alleles.

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Characterization of sunlight-grown transgenic rice plants expressing Arabidopsis phytochrome A

Cited by 28 publications

References 35 publications

Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield

Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield

Rice

Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B

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