Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea: Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori

Yamamoto, Kohji; Kimura, Makoto; Aso, Yoichi; Banno, Yutaka; Koga, Katsumi

doi:10.1303/aez.2007.465

Cited by 3 publications

(5 citation statements)

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“…The apparent specific activity of nlSOD1 O 2 − ‐degradation was 90% of that of bmSOD (Yamamoto et al, 2005b) and 121% of that of hcSOD (Yamamoto et al, 2007) (Table 1). The pH and thermal stability of purified nlSOD1 preparations were investigated.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we discovered and characterized a novel SOD present in N. lugens, named nlSOD1, which possesses N-and C-terminal extensions, compared with bmSOD and hcSOD (Yamamoto et al, 2007(Yamamoto et al, , 2005b. In addition, its mRNA responded to insecticide exposure.…”

Section: Induction Of Nlsod1 Mrna and H 2 O 2 Content After Exposure ...mentioning

confidence: 99%

“…SODs in insects have not been extensively studied compared with those in mammals. SOD1 in Bombyx mori (Yamamoto et al, 2005a) and Hyphantria cunea (Yamamoto et al, 2007), and SOD2 in B. mori (Yamamoto et al, 2005b) have been previously identified. As there have been no reports on SOD in Nilaparvata lugens , in this study, we characterized nlSOD1 in the brown planthopper ( N. lugens ), which is a major pest of rice crops.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a novel superoxide dismutase in Nilaparvata lugens

Yamamoto

Yamaguchi

2021

Arch Insect Biochem Physiol

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The brown planthopper (Nilaparvata lugens) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest-control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn-SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O 2 and H 2 O 2 . Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens. The findings of this study may assist in the development of novel methods to control the population of N. lugens.

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Section: Resultsmentioning

confidence: 99%

Section: Induction Of Nlsod1 Mrna and H 2 O 2 Content After Exposure ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a novel superoxide dismutase in Nilaparvata lugens

Yamamoto

Yamaguchi

2021

Arch Insect Biochem Physiol

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“…Mn-SOD is considered a general stress-responsive element, and several internal and external cues, including pesticide toxicity at the transcriptional and translational levels, can substantially impact its expression profiles ( Zelko et al, 2002 ; Cho et al, 2006 ). The SOD enzymes are a group of highly conserved stress response enzymes that function as molecular chaperones assisting in protein refolding and degradation in stressed cells ( Mayer and Bukau, 2005 ; Yamamoto et al, 2005d; Yamamoto et al, 2007 ). However, only a little is known to date about oxidative stress induced by JGM pesticides and sugars (glucose, sucrose, trehalose) in BPH.…”

Section: Discussionmentioning

confidence: 99%

“…SOD studies in insects are comparatively rare compared to plants and mammals. However, some of the genes have been studied in insects, such as SOD1 in B. mori ( Yamamoto et al, 2005a ) and Hyphantria cunea ( Yamamoto et al, 2007 ), as well as SOD2 in B. mori and NlSOD1 in N. lugens ( Yamamoto and Yamaguchi, 2022 ). In the current study, we have systematically characterized the NlSOD gene family in BPH.…”

Section: Introductionmentioning

confidence: 99%

The superoxide dismutase (SOD) genes family mediates the response of Nilaparvata lugens to jinggangmycin and sugar

Sheraz,

Zhu,

Dong

et al. 2023

Front. Physiol.

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Introduction: Brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), is a major rice pest causing significant damage to rice throughout the world. Intensive pesticide usage often causes resistance in these seasonal pests, mainly through the modulation of antioxidant machinery. The superoxide dismutase (SOD) gene family is known for regulating BPH response to pesticides.Methods: In the present study, we identified eight NlSOD genes from the NCBI using the BLASTP program. The bioinformatics analysis includes a phylogenetic tree, conserved domain, motifs, gene ontology (GO) analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathways, and protein-protein interaction, highlighting the distinctive functional elements of NlSOD genes.Results and discussion: Additionally, the NlSOD genes showed expression in all developmental stages of BPH. Under three sugars (glucose, sucrose, and trehalose) treatment, the respective upregulation of NlSOD8, NlSOD6, and NlSOD2 was noted. The NlSOD1 induced significantly under jinggamycin (JGM) deduced its potential as a key regulator of BPH response to the pesticide. Our study has provided detailed knowledge of the NlSOD gene family in-silico analysis and the defensive response to insecticide and high sugar of BPH. We hope the results of this research will help to shed light on the resistance of BPH towards insecticide toxicity and high sugar and help to control it more efficiently.

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