Characterization of synergistic antibacterial effect of silver nanoparticles and ebselen

Chen, Xueqing; Chen, Heming; Zhang, Hongying; Peng, Yanjuan; Deng, Fuchang; Gao, Jiye; Chai, Chun-Li; Tang, Song; Zuo, Xi‐Nian; Lu, Jun; Du, Hao

doi:10.1080/21691401.2019.1648278

Cited by 12 publications

(11 citation statements)

References 46 publications

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“…As shown in Figure 3b, both AgNPs and AgNPs−PEI−Ce6 can completely kill the E. coli at the testing concentration, which can be ascribed to the higher sensitivity to the Ag + of the Gram-negative bacteria. 43 However, when the concentration of AgNPs was reduced to 0.005 mg mL −1 (Figure S9), the composite AgNPs−PEI−Ce6 can still eliminate the bacteria, while 10 5 cfu mL −1 of E. coli survived from the treatment of AgNPs, demonstrating the superiority of the combined treatment.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

Silver Nanoparticle-Assisted Photodynamic Therapy for Biofilm Eradication

Sun

Tan

et al. 2022

ACS Appl. Nano Mater.

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As an antibiotic-free treatment, photodynamic therapy (PDT) is considered a promising alternative to antibiotic therapy for bacterial infections. However, the recalcitrant bacterial biofilm has manifested significant endurance to PDT, especially the gram-negative bacteria with the protective outer membrane. The ever-developing nanotechnology has provided new opportunities to overcome the biofilm infection. Here, we used silver nanoparticles as the auxiliary for PDT to implement a combined treatment against biofilms. A photosensitizer chlorin e6-modified polyethyleneimine was used as the ligands of silver nanoparticles. In the combined treatment, the silver and PDT exhibited a synergistic effect by mutually reinforcing each other. The surface plasma resonance of silver promotes the photodynamic effect to generate singlet oxygen, and the reactive oxygen can in turn stimulate the oxidative dissolution of the bactericidal Ag + . As a result, the combined treatment showed advanced antibacterial activity against both the Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Moreover, the Gram-negative E. coli, which is more susceptible to silver, becomes almost extinct even in the biofilm form. The therapy on mice with epidermal wound infection verified the high effectiveness of the nanocomposite. This research developed an efficient combined therapy for biofilm eradication, which strengthens the weakness of PDT in eliminating the Gram-negative bacteria, providing an alternative way to fight biofilm-related infections.

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Section: ■ Results and Discussionmentioning

confidence: 97%

Silver Nanoparticle-Assisted Photodynamic Therapy for Biofilm Eradication

Sun

Tan

et al. 2022

ACS Appl. Nano Mater.

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“…Similar to silver ions, silver nanoparticles are strong inhibitors of bacterial thioredoxin reductase and have a synergistic effect on E. coli and S. aureus in vitro in combination with ebselen [ 35 ]. The activity of E. coli thioredoxin reductase decreased in a concentration-dependent manner; an 80% inhibitory effect of nanoparticles was observed at a concentration of 0.54 µg/mL.…”

Section: Synergy Of Ebselen With Silvermentioning

confidence: 99%

Antibacterial Activity of Ebselen

Maślanka

Mucha

2023

IJMS

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Ebselen is a low-molecular-weight organoselenium compound that has been broadly studied for its antioxidant, anti-inflammatory, and cytoprotective properties. These advantageous properties were initially associated with mimicking the activity of selenoprotein glutathione peroxidase, but the biomedical impact of this compound appear to be far more complex. Ebselen serves as a substrate or inhibitor with multiple protein/enzyme targets, whereas inhibition typically originates from the covalent modification of cysteine residues by opening the benzisoselenazolone ring and S–Se bond formation. The inhibition of enzymes of various classes and origins has been associated with substantial antimicrobial potential among other activities. In this contribution, we summarize the current state of the art regarding the antibacterial activity of ebselen. This activity, alone and in combination with commercial pharmaceuticals, against pathogens, including those resistant to drugs, is presented, together with the molecular mechanism behind the reactivity. The specific inactivation of thioredoxin reductase, bacterial toxins, and other resistance factors is considered to have certain therapeutic implications. Synergistic action and sensitization to common antibiotics assisted with the use of ebselen appear to be promising directions in the treatment of persistent infections.

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“…Several studies have shown that application of exogenous natural AMPs can have immunomodulatory and protective effects against infectious keratitis ( Gao and Yu, 2015 ; Kolar et al, 2015 ; Shah et al, 2017 ). Furthermore, it has been observed that AMPs can synergize with each other and additional antibacterial enzymes ( Nagaoka et al, 2000 ; Singh et al, 2000 ; Wu et al, 2009 ; Chen et al, 2019 ). From this, researchers hypothesized that AMPs could synergize with current antibiotic therapies to potentiate bactericidal effects ( Li et al, 2014 ; Carion et al, 2020 ).…”

Section: Therapeutic Use Of Antimicrobial Peptidesmentioning

confidence: 99%

Antimicrobial Peptide Expression at the Ocular Surface and Their Therapeutic Use in the Treatment of Microbial Keratitis

et al. 2022

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Microbial keratitis is a common cause of ocular pain and visual impairment worldwide. The ocular surface has a relatively paucicellular microbial community, mostly found in the conjunctiva, while the cornea would be considered relatively sterile. However, in patients with microbial keratitis, the cornea can be infected with multiple pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, and Fusarium sp. Treatment with topical antimicrobials serves as the standard of care for microbial keratitis, however, due to high rates of pathogen resistance to current antimicrobial medications, alternative therapeutic strategies must be developed. Multiple studies have characterized the expression and activity of antimicrobial peptides (AMPs), endogenous peptides with key antimicrobial and wound healing properties, on the ocular surface. Recent studies and clinical trials provide promise for the use of AMPs as therapeutic agents. This article reviews the repertoire of AMPs expressed at the ocular surface, how expression of these AMPs can be modulated, and the potential for harnessing the AMPs as potential therapeutics for patients with microbial keratitis.

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Characterization of synergistic antibacterial effect of silver nanoparticles and ebselen

Cited by 12 publications

References 46 publications

Silver Nanoparticle-Assisted Photodynamic Therapy for Biofilm Eradication

Silver Nanoparticle-Assisted Photodynamic Therapy for Biofilm Eradication

Antibacterial Activity of Ebselen

Antimicrobial Peptide Expression at the Ocular Surface and Their Therapeutic Use in the Treatment of Microbial Keratitis

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