Characterization of tachykinin receptors in the uterus of the oestrogen‐primed rat

Magraner, Josefina; Pinto, Francisco M.; Anselmi, Elsa; Hernández, Mariano; Perez-Afonso, Ricardo; Martı́n, Julio D.; Advenier, C.; Candenas, Luz

doi:10.1038/sj.bjp.0701613

Cited by 27 publications

(43 citation statements)

References 39 publications

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“…The order of potency of the mammalian tachykinins (NKA4SPXNKB); the actions of receptorselective agonists; and the potent antagonistic effect of the tachykinin NK 2 receptor-selective antagonist, SR48968 (Emonds-Alt et al, 1992), all pointed towards the notion that the NK 2 receptor mediates the uterotonic effects of tachykinins. These findings are similar to those in rat uterus (Fisher et al, 1993;Pennefather et al, 1993b;Fisher & Pennefather, 1997;Magraner et al, 1998), but are in contrast to the nonpregnant mouse in which NK 1 receptors are important in mediating uterine contraction (Patak et al, 2002a).…”

Section: E Patak Et Alsupporting

confidence: 73%

“…To date, the majority of molecular (Pinto et al, 1999;Candenas et al, 2001), immunohistochemical (Traurig et al, 1984;Papka et al, 1985) and functional (Fisher et al, 1993;Pennefather et al, 1993b;Fisher & Pennefather, 1997;Magraner et al, 1998;Patak et al, 2000a) studies of the uterine distribution of tachykinins, tachykinin receptors and/or degradation of tachykinins have been conducted using the rat. It is now emerging, however, that there are species (Patak et al, 2000a, b;2002a) as well as hormonal-and pregnancyrelated differences in tachykinin expression and actions in the mammalian myometrium (Pinto et al, 1999;Candenas et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Tachykinins and tachykinin receptors in human uterus

Patak

Candenas

Pennefather

et al. 2003

British J Pharmacology

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1 Studies were undertaken to determine the nature of the receptors mediating contractile effects of tachykinins in the uteri of nonpregnant women, and to analyse the expression of preprotachykinins (PPT), tachykinin receptors and the cell-surface peptidase, neprilysin (NEP), in the myometrium from pregnant and nonpregnant women. 2 The neurokinin B (NKB) precursor PPT-B was expressed in higher levels in the myometrium from nonpregnant than from pregnant women. Faint expression of PPT-A mRNA was detectable in the myometrium from nonpregnant but not pregnant women. PPT-C, the gene encoding the novel tachykinin peptide hemokinin-1 (HK-1), was present in trace amounts in the uteri from both pregnant and nonpregnant women. 3 Tachykinin NK 2 receptors were more strongly expressed in tissues from nonpregnant than from pregnant women. NK 1 receptor mRNA was present in low levels in tissues from both pregnant and nonpregnant women. A low abundance transcript corresponding to the NK 3 receptor was present only in tissues from nonpregnant women. 4 The mRNA expression of the tachykinin-degrading enzyme NEP was lower in tissues from nonpregnant than from pregnant women. 5 Substance P (SP), neurokinin A (NKA) and NKB, in the presence of the peptidase inhibitors thiorphan, captopril and bestatin, produced contractions of myometrium from nonpregnant women. The order of potency was NKAbSPXNKB. The potency of NKA was unchanged in the absence of peptidase inhibitors. Nle 10 ]NKA(4 -10). 8 These data are consistent with a role of tachykinins in the regulation of human uterine function, and reinforce the importance of NK 2 receptors in the regulation of myometrial contraction.

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Section: E Patak Et Alsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Tachykinins and tachykinin receptors in human uterus

Patak

Candenas

Pennefather

et al. 2003

British J Pharmacology

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“…Preparation of uterine strips was done as previously described [20] with slight modifications as follows. Uterine horns from virgin female Wistar rats (200 ± 250 g) in the oestrous stage of the hormonal cycle were removed and carefully cleaned.…”

Section: Experimental Partmentioning

confidence: 99%

“…Neither 1 nor the mixture 1 ± 3 of natural composition had any significant effect on preparations of rat-isolated uterus under resting tension ( Table, a). In the same myometrial strips, a maximally effective concentration of acetylcholine (ACh) (10 À3 m) [20] elicited an initial, rapid phasic contraction (83.51 AE 6.83, expressed, here and below, in mg per mg of wet tissue) followed by rhythmic contractions. The ACh-induced contraction was completely abolished in the presence of atropine (10 À6 m), demonstrating that the O-galactosyldi-O-acylglycerolipids assayed here are devoid of cholinergic activity.…”

mentioning

confidence: 99%

Polar Metabolites of the Tropical Green SeaweedCaulerpa taxifolia Which Is Spreading in the Mediterranean Sea: Glycoglycerolipids and Stable Enols (α\=Keto Esters)

Mancini

Guella

Defant

et al. 1998

HCA

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Examination of the polar components of the green seaweed Caulerpa taxifolia (Vahl) C. Agardh, which is heavily spreading in the northeastern Mediterranean, led to two families of compounds. The new (2R)‐3‐O‐β‐D‐galactopyranosyl‐1‐O‐[(7Z,10Z,13Z)‐hexadeca‐7,10,13‐trienoyl]‐2‐O‐[(9Z,12Z,15Z)‐octadeca‐1,12,15‐trienoyl]‐sn‐glycerol (2) was isolated in low abundance, like the analogues 1 and 3 already known from freshwater cyanobacteria. The acyl positions in 1 – 3 were determined by enzymatic methods and the absolute configuration from the O‐galactosylglycerol obtained upon alcaline methanolysis. More abundant were the (4‐hydroxyphenyl)‐ and (3,4‐dihydroxyphenyl)pyruvic acid methyl esters, occurring in the enol (Z) forms 13a and 14a accompanied by very minor (E) forms 13b and 14b. The latter became predominant on UV irradiation of 13a or 14a, allowing the determination of the C=C configuration of these isolatable, stable enols from 1H,13C NMR couplings (larger H−C(3)/C(1) coupling constant in the (E) than in the (Z) isomer). Contrary to literature implications, the O‐galactosylglycerolipids 1 – 3 lack any cholinergic or histaminergic activity; similarly, enols (= α‐keto esters) 13 and 14 or terpenoids of this seaweed were also devoid of such biological activities (see Table).

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“…The majority of studies of TKs and their effects and their receptors on the mammalian uterus have been undertaken using the non-pregnant rat (Shew et al, 1991;Barr et al, 1991;Fisher et al, 1993;Pennefather et al, 1993;Fisher and Pennefather, 1997, 1999Magraner et al, 1997Magraner et al, , 1998Moodley et al, 1999;Hamlin et al, 2000;Patak et al, 2000a;Shintani et al, 2000;Cintado et al, 2001;Pinto et al, 2001;Crane et al, 2002;Williams et al, 2003). SP, NKA, and NKB all cause concentration-dependent contraction of the longitudinally arranged myometrium from cycling (Moodley et al, 1999) and oestrogen-primed virgin rats Magraner et al, 1998;Fisher and Pennefather, 1999).…”

Section: Effects On Myometrial and Oviductal Contractilitymentioning

confidence: 98%