Characterization of Tanzanian elite sweet potato genotypes for sweet potato virus disease (SPVD) resistance and high dry matter content using simple sequence repeat (SSR) markers

Gwandu, C.; Tairo, Fred; Mneney, Emmarold E.; Kullaya, Alois

doi:10.5897/ajb11.4208

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…This suggestion might be supported by Dominguaz [20] who reported that dry matter content is genetically controlled trait in sweet potato. Catherine and Scott [21,22] reported that the dry matter percentage of different sweet potato varieties were between 13.4 and 29.2%, 25.23 and 41.11%, respectively. Tsakama, Fred, Bonsi and Loretan [23][24][25][26] also found that the dry matter in storage roots were ranged from 12.5 to 30.2, 29 to 39.07, 25 to 42 and 25.5 to 31.7%, respectively.…”

Section: Marketable Unmarketable and Total Storage Root Numbermentioning

confidence: 99%

Agronomic and Physicochemical Evaluation of Sweet Potato [Ipomoea batatas (L.) Lam.] Collections in Ethiopia

Mohammed¹

2015

Adv Crop Sci Tech

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The productivity of sweet potato [Ipomoea batatas (L.) Lam.] is mainly dependant on the acquisition accessions which posses desirable traits and development of high yielding varieties with desired quality attributes. For this purpose, Haramaya University collected 116 sweet potato accessions from International and National sources to develop varieties for eastern Ethiopia; however, the accessions characterization and documentation were not exhaustively done to support the improvement program. Therefore, this study was conducted during 2012/2013 cropping season to characterize, evaluate, and documenting of agronomic and physicochemical attributes of sweet potato accessions at Haramaya. Augmented design consisting of 114 entries/tests and two checks were used. Varied number of accessions recorded significantly higher values than the mean of the checks for days to physiological maturity, above ground fresh biomass, storage root fresh weight, total storage root yield, marketable storage root yield, reducing sugar, total sugar, and total starch content, pH, dry matter content, total soluble solid, specific gravity and peel content. Tis-9465-7 had the highest storage root fresh weight yield; marketable storage root yield and total storage root yield and Koka-12 and CN-2069-7 exhibited significantly highest values than mean of the checks for days to physiological maturity and above ground fresh biomass, respectively. CN-1752-14, CN-2056 and Tis-80/043-1 for reducing sugar, pH and total soluble solid, respectively, exhibited significantly highest values, while CN-1752-15 recorded the highest total sugar and total starch content. Korojo had significantly highest values for specific gravity, dry matter. Tis-82/0602 were exhibited that the lowest in peel content. Elliptic shape (27.19%) and horizontal constriction (45.62%) defect were dominant in the accessions. Most of the accession had white skin color (22.6%) while 21.92% accessions had creamy flesh color.

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Section: Marketable Unmarketable and Total Storage Root Numbermentioning

confidence: 99%

Agronomic and Physicochemical Evaluation of Sweet Potato [Ipomoea batatas (L.) Lam.] Collections in Ethiopia

Mohammed¹

2015

Adv Crop Sci Tech

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“…Similarly, Gwandu et al (2012) analyzed 57 sweet potato genotypes in Tanzania with 4 SSRs and found alleles from 11 to 22.…”

Section: Genetic Variabilitymentioning

confidence: 99%

Molecular genotyping of sweet potato (Ipomoea batatas L. Lam) accessions using microsatellites

Keyagha

Ulasi

Umeh

et al. 2023

Arch. Agric. Environ. Sci.

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The experiment was conducted to ascertain the level of genetic diversity in sweet potato accessions using microsatellites. Thirty sweet potato accessions obtained from the International Potato Center (CIP), Kumasi, Ghana, Mozambique, and local germplasm of the National Root Crops Research Institute (NRCRI), Umudike, Abia State, Nigeria, as well as sweet potato vines from local farmers' fields in Jos, Plateau State, and Bauchi State, Nigeria, were analyzed for genetic diversity using five microsatellite markers. The results showed that the polymorphic SSR loci revealed diverse relationship among the sweet potato cultivars, which was grouped into four major clusters by unweighted pair group method analysis (UPGMA) method. Cluster analysis showed a Jaccard co-efficient ranging from 0.0 to 3.0 indicating high genetic diversity. The primers detected a total of 18 alleles and the number of alleles per locus was 4 for IBR-19, IBR-286, IBR-297 and 3 for IBR-16 and IBR-242 with an average of 3.67 alleles per locus. The polymorphic information content (PIC) of the markers varied from 0.35 to 0.72 with an average of 0.497. Marker IBR-19 revealed the highest PIC of 0.72, while marker IBR-297 had the lowest PIC of 0.35. Observed heterozygosity ranged from 0.32 to 0.89 with a mean of 0.675 across the five SSR loci. The results from the Analysis of molecular variance (AMOVA) which was used to quantify the diversity level and genetic relationship among the thirty sweet potato accessions indicated that a high diversity was mostly distributed within the populations for sweet potato accessions (75.12%) and (15.67%) among the populations.

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“…In that study, 255 alleles were identified at 21 loci with an observed heterozygosity of 0.71 across populations. Gwandu et al (2012) examined diversity within 57 Tanzanian elite sweetpotato genotypes using four SSR markers. The results showed no specific clustering based on disease, dry matter content, or geographic location.…”

Section: Introductionmentioning

confidence: 99%

Genetic Identity, Diversity, and Population Structure of CIP's Sweetpotato (I. batatas) Germplasm Collection

et al. 2021

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The in trust sweetpotato collection housed by the International Center of Potato (CIP) is one of the largest assemblages of plant material representing the genetic resources of this important staple crop. The collection currently contains almost 6,000 accessions of Ipomoea batatas (cultivated sweetpotato) and over 1,000 accessions of sweetpotato crop wild relatives (CWRs). In this study, the entire cultivated collection (5,979 accessions) was genotyped with a panel of 20 simple sequence repeat (SSR) markers to assess genetic identity, diversity, and population structure. Genotyping and phenotyping of in vitro plantlets and mother plants were conducted simultaneously on 2,711 accessions (45% of the total collection) to identify and correct possible genetic identity errors which could have occurred at any time over the thirty plus years of maintenance in the in vitro collection. Within this group, 533 accessions (19.6%) had errors in identity. Field evaluations of morphological descriptors were carried out to confirm the marker data. A phylogenetic tree was constructed to reveal the intraspecific relationships in the population which uncovered high levels of redundancy in material from Peru and Latin America. These genotypic data were supported by morphological data. Population structure analysis demonstrated support for four ancestral populations with many of the accessions having lower levels of gene flow from the other populations. This was especially true of germplasm derived from Peru, Ecuador, and Africa. The set of 20 SSR markers was subsequently utilized to examine a subset of 189 accessions from the USDA sweetpotato germplasm collection and to identify and reconcile potential errors in the identification of clones shared between these collections. Marker analysis demonstrated that the USDA subset of material had 65 unique accessions that were not found in the larger CIP collection. As far as the authors are aware, this is the first report of genotyping an entire sweetpotato germplasm collection in its entirety.

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Characterization of Tanzanian elite sweet potato genotypes for sweet potato virus disease (SPVD) resistance and high dry matter content using simple sequence repeat (SSR) markers

Cited by 11 publications

References 19 publications

Agronomic and Physicochemical Evaluation of Sweet Potato [Ipomoea batatas (L.) Lam.] Collections in Ethiopia

Agronomic and Physicochemical Evaluation of Sweet Potato [Ipomoea batatas (L.) Lam.] Collections in Ethiopia

Molecular genotyping of sweet potato (Ipomoea batatas L. Lam) accessions using microsatellites

Genetic Identity, Diversity, and Population Structure of CIP's Sweetpotato (I. batatas) Germplasm Collection

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