Internal temperature monitoring of battery cells can be very useful, as the core temperature can deviate significantly from that of the housing, especially in case of cells with a thick electrode stack. Conventional resistance temperature detectors can accurately measure temperature, but are limited to the outer surface of the cell due to induction effects. They are therefore not suitable for internal in situ measurements. Fiber Bragg grating (FBG) sensors are unaffected by the electric field as they operate by reflecting light. However, a specific difficulty is the distinction of temperature vs. strain effects as the grating is sensitive to both. In this work a calibration routine to separate the influences of temperature and strain in a lithium-ion battery cell is presented and examined for two multi-layer stack pouch cells (10 and 20 Ah). The obtained in situ temperature data reveal a difference of up to 2°C between center and cell housing at elevated discharge rate (4C) and a delay in detection of temperature peaks by the external sensor by 12 s. Strain data correlate with numbers of electrode layers in the stack and yield a stress of up to 27.3 MPa in the center of the 20 Ah cell.