Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection

Sandallı, Cemal; Özgümüş, Osman Birol; Sevim, Ali

doi:10.1007/s11274-010-0381-z

Cited by 12 publications

(10 citation statements)

References 20 publications

(12 reference statements)

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“…The presence of tet(B), tet(E) and, rarely, tet(D) determinants has also been noted in TET R and OTC R isolates, whereas the tet(C) gene was not found. Similar results were reported by Börjesson et al [36], Sandalli et al [34] and Tao et al [19]. Auerbach et al [37] observed a wide variety of tetracycline resistance genes in wastewater, including tet(C) which was not identified in this study.…”

Section: Discussionsupporting

confidence: 91%

“…In untreated wastewater isolates, there was not the predominant gene in the TET R group, tet(A) was most frequently determined in the OTC R groups, whereas the predominance of tet(A) gene was noted in both bacterial groups from treated effluent. Sandalli et al [34] and Tao et al [19] have demonstrated the predominance of the tet(A) gene in Enterobacteriaceae bacteria, and Harnisz et al [14] -in bacteria of Aeromonas genus. The tet(A) gene, which encodes the removal of tetracyclines from the cell with the involvement of transport proteins, is often found on plasmids, including conjugating plasmids [35].…”

Section: Discussionmentioning

confidence: 99%

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Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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Abstract. The objective of this study was to monitor changes (amplification or attenuation) in antibiotic resistance during wastewater treatment based on the ecology of tetracycline-resistant bacteria. The untreated and treated wastewater were collected in four seasons. Number of tetracycline-(TET R ) and oxytetracycline-resistant (OTC R ) bacteria, their qualitative composition, minimum inhibitory concentrations (MICs), sensitivity to other antibiotics, and the presence of tet(A, B, C, D, E) resistance genes were determined. TET R and OTC R counts in untreated wastewater were 100 to 1000 higher than in treated effluent. OTC R bacterial counts were higher than TET R populations in both untreated and treated wastewater. TET R isolates were not dominated by a single bacterial genus or species, whereas Aeromonas hydrophila and Aeromonas sobria were the most common in OTC R isolates. The treatment process attenuated the drug resistance of TET R bacteria and amplified the resistance of OTC R bacteria. In both microbial groups, the frequency of tet(A) gene increased in effluent in comparison with untreated wastewater. Our results also indicate that treated wastewater is a reservoir of multiple drug-resistant bacteria as well as resistance determinants which may pose a health hazard for humans and animals when released to the natural environment.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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“…Tetracycline is the most frequently used antibiotic in veterinary medicine and in aquaculture worldwide (Miranda 2012). Sandalli et al (2010) reported that bacteria isolated from rivers of the Black Sea region of Turkey had tetA and tetB resistance genes among tested tet genes. DePaola & Roberts (1995) isolated and characterized tetracycline-resistant Gram-negative bacteria from catfish and reported that these isolates harbored either the tetD or tetE gene.…”

Section: T2mentioning

confidence: 99%

Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment

Çapkın¹,

Terzi̇²,

Altınok³

2015

Dis. Aquat. Org.

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Antibiotic resistance and presence of the resistance genes were investigated in the bacteria isolated from water, sediment, and fish in trout farms. A total of 9 bacterial species, particularly Escherichia coli, were isolated from the water and sediment samples, and 12 species were isolated from fish. The antimicrobial test indicated the highest resistance against sulfamethoxazole and ampicillin in coliform bacteria, and against sulfamethoxazole, imipenem, and aztreonam in known pathogenic bacteria isolated from fish. The most effective antibiotics were rifampicin, chloramphenicol, and tetracycline. The multiple antibiotic resistance index was above the critical limit for almost all of the bacteria isolated. The most common antibiotic resistance gene was ampC, followed by tetA, sul2, blaCTX-M1, and blaTEM in the coliform bacteria. At least one resistance gene was found in 70.8% of the bacteria, and 66.6% of the bacteria had 2 or more resistance genes. Approximately 36.54% of the bacteria that contain plasmids were able to transfer them to other bacteria. The plasmid-mediated transferable resistance genes were ampC, blaCTX-M1, tetA, sul2, and blaTEM. These results indicate that the aquatic environment could play an important role in the development of antibiotic resistance and the dissemination of resistance genes among bacteria.

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“…Currently, tetracycline resistance genes have been widely selected as an indicator to evaluate ARGs contamination [21][22][23] . Among the four kinds of tetracycline resistance genes (tetC, tetG, tetM, and tetO) tested in this study, tetC was detected in all sampling sites (both water and sediment) and tetG was also detected in all water samples which was consistent with previous studies [1,22,[24][25][26] .…”

Section: Discussionmentioning

confidence: 99%

Occurrence of antibiotic resistance and integronase genes in Taihu Lake

Wang¹,

Pu²,

Ye³

et al. 2016

Appl Env Biotechnol

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Antibiotic resistance genes (ARGs), a potential threat to the health of humans and animals, have been widely detected in various environments. However, not much information about ARGs in freshwater lakes have been recorded. In this study, we investigated the occurrence of 17 kinds of ARGs and three types of integronase genes in Taihu Lake (China), an important drinking water source for local residents. Fecal coliforms were also isolated from the water and sediments for antimicrobial susceptibility tests and related ARGs detection. Results showed that tetracycline resistance gene tetC, sulfanilamide resistance genes sul1 and sul2, and class 1 integronase gene int1 were present in all water and sediment samples. TetG was present in all water samples but was mainly distributed in sediment samples from the northern region of Taihu Lake. β-Lactam resistance gene blaOXA-1 was present in all water samples but was absent in the sediment samples. TetM and tetO were found present in water and sediment samples from the western area of the lake. Remarkably, 95% of isolated fecal coliforms were resistant to trimethoprim and multi-drug resistant isolates were also observed. Sul1 and tetC genes were found to be carried by isolates resistant to corresponding antibiotics. This study provided baseline information about the occurrence of ARGs and integronase genes in Taihu Lake and the results may extend our knowledge about antibiotic resistance of microbial communities in the lake.

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Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection

Cited by 12 publications

References 20 publications

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment

Occurrence of antibiotic resistance and integronase genes in Taihu Lake

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