Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh

Binnie, J.; McManus, Michael T.

doi:10.1016/j.phytochem.2009.01.002

Cited by 47 publications

(36 citation statements)

References 61 publications

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“…In apple, ACO genes are encoded by a multigene family, and three isoforms have been reported so far, namely ACO1, ACO2 and ACO3 identified by Binnie and McManus (2009); all three gene isoforms are differentially expressed in fruit and leaf tissue. Of these ACO isoforms, ACO1 is predominantly expressed in ripening apple fruit (Wakasa et al 2006).…”

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confidence: 99%

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Maric¹

2016

Hort. Sci. (Prague)

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Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This paper presents the results of molecular cloning and characterization of a newly identified allele of the ripening-specific ACO1 gene from Malus floribunda 821. A polymorphism of the ACO1 gene was detected using the polymerase chain reaction and BamH1 and RsaI enzymatic digestion. Whilst cloning and sequencing data confirmed the identity of allele d, it also revealed high sequence conservation (97.3 to 98.9%). In addition, a comparison of sequence data revealed some differences within the coding regions of the ACO1 alleles identified so far.

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confidence: 99%

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Maric¹

2016

Hort. Sci. (Prague)

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“…Western blot analysis was performed in a wheat germ protein expression system using anti-Flag raised against the gene product of AsACO. The molecular mass of the protein was similar to that of MDACO3 (Binnie and McManus, 2009). …”

Section: Discussionmentioning

confidence: 61%

“…Signaling regulated by ethylene is involved in plant growth, including seed germination, flower and leaf formation, sexual development, fruit ripening, and senescence (Bleecker and Kende, 2000). Ethylene biosynthesis has been demonstrated to play a crucial role not only in plant development, but also in stress tolerance (Yang and Hoffman, 1984;Binnie and McManus, 2009). Therefore, studies have investigated the mechanisms of ethylene production and manipulated the amount of ethylene produced, thus increasing plant vigor and stress tolerance (Ski and Ska, 2005;Han et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Much research has been performed to isolate and utilize the ACO gene in dicotyledons, such as apple (Binnie and McManus, 2009), melon (Lasserre et al, 1996), white clover (Chen and McManus, 2006), and the model organism Arabidopsis thaliana (Babula et al, 2006). However, limited studies have been performed using monocotyledonous plants such as Agrostis stolonifera, to explore the mechanisms controlling ethylene biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Cloning and expression of the 1-aminocyclopropane-1-carboxylic oxidase gene from Agrostis stolonifera

Xiao¹,

Li²,

Teng³

et al. 2016

Genet. Mol. Res.

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ABSTRACT.A gene encoding 1-aminocyclopropane-1-carboxylic oxidase (ACO), which catalyzes the terminal step in ethylene biosynthesis, was isolated from Agrostis stolonifera. The AsACO gene is composed of 975 bp, encoding 324 amino acids. Three exons interspersed by two introns form AsACO gDNA. A BLAST search of the nucleotide sequence revealed a high level of similarity (79-91%) between AsACO and ACO genes of other plants. A phylogenetic tree was constructed via BLAST in the NCBI, and revealed the highest homology with wheat TaACO. The calculated molecular mass and predicted isoelectric point of AsACO were 36.25 and 4.89 kDa, respectively. Analysis of subcellular localization revealed that AsACO is located in the nucleus and cytoplasm. The Fe(II)-binding cofactors and cosubstrate were identified, pertaining to the ACO family. The expression patterns of AsACO were determined by quantitative real time PCR. AsACO expression was highest in the stem, and was strongly up-regulated in response to ethephon, methyl jasmonate, salicylic acid, and cold temperature, but down-regulated in response to drought and NaCl treatment. The protein encoded by AsACO exhibited ACC oxidase activity in vitro. Taken together, these findings suggest that AsACO contains domains common to the ACO family, and is induced in response to exogenous hormones. Conversely, some abiotic stress conditions can inhibit AsACO expression.

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“…ACO has been cloned and characterized in many plant species such as peach, potato, strawberry, white clover, plum, Arabidopsis, rice, apple, pine, pear (Ruperti et al, 2001;Nie et al, 2002;Trainotti et al, 2005;Chen and McManus, 2006;Fernandez-Otero et al, 2006;Babula et al, 2006;Quyang et al, 2007;Binnie and McManus, 2009;Yuan et al, 2010;Qi et al, 2015). Here, we identified the GbACO gene originated from the G. bilobaleaves that promoted the growth of ginkgo, and analyzed the sequence structure and function.We constructed a phylogenetic tree for the comparison of GbACO with other members of the plant dioxygenase family through the neighbor-joining method.…”

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confidence: 99%

Cloning and Identification of an ACO Gene Involved in Ethylene Production Pathway from Ginkgo biloba

Yan¹,

Zhang²,

Feng³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh

Cited by 47 publications

References 61 publications

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Molecular cloning and characterization of allele d ‒ a newly identified allele of the 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) gene in apple - Short Communication

Cloning and expression of the 1-aminocyclopropane-1-carboxylic oxidase gene from Agrostis stolonifera

Cloning and Identification of an ACO Gene Involved in Ethylene Production Pathway from Ginkgo biloba

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