Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI)

Walker, James I. H.; Faik, Pelin; Morgan, Michael J.

doi:10.1016/0888-7543(90)90212-d

Cited by 25 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The long distance between this TATA element and its transcription start site is a clear example that TATA elements can function at considerable upstream distances. Other promoters contain TATA elements as far as 100 bp 5' to their transcription start sites, although not all have been examined for functional promoter activity (27)(28)(29)(30).…”

Section: Resultsmentioning

confidence: 99%

Functional identification of the promoter for the gene encoding the alpha subunit of calcium/calmodulin-dependent protein kinase II.

Olson¹,

Massé²,

Suzuki³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

To examine the expression of the alpha subunit of calcium/calmodulin-dependent protein kinase II, various 5' flanking genomic sequences were inserted into a chloramphenicol acetyltransferase (CAT) reporter plasmid and CAT enzyme activities were analyzed in transfected NB2a neuroblastoma cells and mRNA transcription was analyzed by nuclease protection assays. A core promoter was identified which contained an essential TATA element located 162 nt 5' to the transcription start site. Sequences 3' to the transcription start site, as well as 5' to the TATA element, increased levels of CAT activity in transfected cells. The alpha-subunit gene promoter displayed higher CAT activities, relative to a simian virus 40 promoter, in transfected neuronal cell lines than in nonneuronal cell lines. Results also suggested that sequence surrounding the natural alpha-gene transcription initiation site may be important for targeting transcription initiation 162 nt downstream of its TATA element.

show abstract

Section: Resultsmentioning

confidence: 99%

Functional identification of the promoter for the gene encoding the alpha subunit of calcium/calmodulin-dependent protein kinase II.

Olson¹,

Massé²,

Suzuki³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…A short genomic sequence has been reported for human PGI [42] that includes the two 5' exons and part of the intervening intron of ca. 1 kb.…”

Section: Comparison Of C Lewisii Pgic2-a To Other Pgismentioning

confidence: 99%

Molecular analysis of the plant gene encoding cytosolic phosphoglucose isomerase

Thomas

Laudencia‐Chingcuanco

Gottlieb

1992

Plant Mol Biol

View full text Add to dashboard Cite

The gene encoding a cytosolic isozyme of phosphoglucose isomerase (PGI, EC 5.3.1.9) was isolated from Clarkia lewisii, a wild flower native to California, and the structure and sequence of the entire coding region determined. PGI catalyzes an essential step in glycolysis and carbohydrate biosynthesis in plants. Spanning about 6 kb, the gene has 23 exons and 22 introns, the highest number yet reported in plants. The exons range in size from 43 to 156 nt and encode a protein of 569 amino acids. The protein is about 44-46% identical to the inferred protein sequences of pig, Escherichia coli and Saccharomyces cerevisiae. All of the introns are bordered with the consensus 5'-GT...AG-3' dinucleotides. The longest intron includes a large stem-loop structure bounded by a perfect 9 nt direct repeat. We cloned the PGI gene from a genomic library prepared from a single plant of known PGI genotype. The locus and allele of the clone were identified by matching restriction fragments to fragments from genetically defined genomic DNAs by Southern hybridization.

show abstract

“…The 1.9-kb GPI cDNA predicts a protein of 558 residues. The sequence of the 5' noncoding region, exon 1, intron 1, and exon 2 of the GPI gene has been reported and data suggest that the gene is at least 50 kb in length ( 19). Recently, two patients with chronic hemolytic anemia caused by GPI deficiency were shown by reverse transcription PCR to exhibit point mutations (20 for 30 s were performed in a Thermalcycler (Perkin-Elmer Corp., Norwalk, CT).…”

Section: Introductionmentioning

confidence: 99%

The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic anemia.

Xu¹,

Beutler²

1994

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI)

Cited by 25 publications

References 18 publications

Functional identification of the promoter for the gene encoding the alpha subunit of calcium/calmodulin-dependent protein kinase II.

Functional identification of the promoter for the gene encoding the alpha subunit of calcium/calmodulin-dependent protein kinase II.

Molecular analysis of the plant gene encoding cytosolic phosphoglucose isomerase

The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic anemia.

Contact Info

Product

Resources

About