Characterization of the analytic performance of an electrochemical point‐of‐care meter for measuring β‐hydroxybutyrate concentration in blood and plasma from periparturient dairy cattle

Abstract

Meter accuracy was markedly dependent on the BHBe-to-BHBp ratio and consequently the HCT. Therefore, the algorithm used by the meter should be revised when applied to bovine blood for improved accuracy.

Clinical Utility of Plasma Fructosamine Concentration as a Hypoglycemic Biomarker during Early Lactation in Dairy Cattle

“…The study reported here was part of a larger study investigating energy and potassium homeostasis in the periparturient period, and the prediction of parturition and dystocia in Holstein–Friesian cattle. These results have been published elsewhere …”

“…Immediately after blood collection, blood [BHB] was measured in a drop of non‐heparinized blood from the tip of the vacutainer needle using Precision Xtra point‐of‐care meter . Plasma [BHB] was calculated from the Precision Xtra reading for blood [BHB] in mmol/L ([BHB] b‐meter ) using the following equation developed by our laboratory: …”

“…Body condition score (BCS) and backfat thickness (BFT) were measured to assess fat mobilization and LDT was measured to assess muscle mobilization; these are well validated indices that assess the magnitude of negative energy balance in early lactation . Periparturient dairy cows initially mobilize skeletal muscle in response to negative energy balance in order to provide amino acids for gluconeogenesis.…”

Evaluation of a point‐of‐care electrochemical meter to detect subclinical ketosis and hypoglycaemia in lactating dairy cows

“…Few method validation studies document the performance of the Precision Xtra® meter to measure blood BHB or glucose in cattle. Those validation studies applied a drop of blood to the test strip, which resulted in unavoidable variability in the applied blood volume and therefore the moles of BHB or glucose potentially available for chemical reaction.…”

“…The current version of the Precision Xtra® meter uses glucose oxidase as the active enzyme in the test strip reaction, whereas an earlier version used glucose dehydrogenase. The manufacturers of the current meters recommend that glucose oxidase reference methodology be used in method comparison studies instead of the hexokinase assay method that was used in the three previously published method comparison studies and may have resulted in an underestimation of the meter's accuracy. Our second hypothesis was therefore that the Precision Xtra® meter's performance is improved when the glucose‐oxidase reference method is applied.…”