Characterization of the antigenic and functional domains of a Mycoplasma synoviae variant vlhA gene

Khiari, A. Béjaoui; Mardassi, Boutheina Ben Abdelmoumen

doi:10.1016/j.vetmic.2011.11.016

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…Elongation factor-Tu (EF-Tu) is one of the most abundant and conserved bacterial proteins and plays a key role in protein synthesis ( 33 ). MSPB is the N-terminal part of vlhA that is responsible for the attachment of MS to chicken erythrocytes ( 34 ). MSPB and LP78 are major MS lipoproteins ( 18 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

“…Thirdly, the divergent antigenic variations can protect MS against elimination by the host’s immune system. vlhA is the most abundant and important virulence factor of MS , but it undergoes phase variable expression, and highly divergent antigenic variants are generated owing to pseudogene insertions ( 34 , 45 ). Additionally, the inoculum of NDV-IBV vaccine priors to MS exposure exacerbates the pathogenicity of MS infection ( 3 , 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Adhesion to host cells is a prerequisite for mycoplasma infection and a precondition for successful colonization (11). In previous studies, antibodies against recombinant DnaK, enolase, EF-Tu, MSPB and NADH can inhibit mycoplasmas adherence and growth (20,21,23,34,35). Thus, the humoral immune responses are assumed to contribute to the protective immunity.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae

Han,

Wang,

Chang

et al. 2024

Front. Vet. Sci.

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Mycoplasma synoviae (MS) is a primary avian pathogen prevalent worldwide that causes airsacculitis and synovitis in birds. Vaccination is recommended as the most cost-effective strategy in the control of MS infection. Novel alternative vaccines are needed for eradicating and controlling MS infection in flocks. DnaK, enolase, elongation factor Tu (EF-Tu), MSPB, NADH oxidase and LP78 are the major immunogenic antigens of MS and are promising targets for subunit vaccine candidates. In the present study, genes encoding DnaK, enolase, EF-Tu, MSPB, LP78, and NADH oxidase were cloned and expressed in Escherichia coli. Enzyme-linked immunosorbent assay showed that the six recombinant proteins were recognized by convalescent sera, indicating that they were expressed during infection. Two injections of the six subunit vaccines induced a robust antibody response and increased the concentrations of IFN-γ and IL-4, especially rEnolase and rEF-Tu. The proliferation of peripheral blood lymphocytes was enhanced in all of the immunized groups. Chickens immunized with rEnolase, rEF-Tu, rLP78, and rMSPB conferred significant protection against MS infection, as indicated by significantly lower DNA copies in the trachea, lower scores of air sac lesions, and lesser tracheal mucosal thickness than that in the challenge control. Especially, rEnolase provided the best protective efficacy, followed by rEF-Tu, rMSPB, and rLP78. Our finds demonstrate that the subunit vaccines and bacterin can only reduce the lesions caused by MS infection, but not prevent colonization of the organism. Our findings may contribute to the development of novel vaccine agents against MS infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae

Han,

Wang,

Chang

et al. 2024

Front. Vet. Sci.

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“…The protein family Variable lipoprotein haemagglutinin was identi ed as an attachment by MS. It encoded two major immunodominant and surface-exposed membrane proteins, an amino-terminal region (MSPB) and a carboxyl-terminal region (MSPA) [29,30]. MSPA has been reported involvement in the adhesion of MS to host cells, and MSPB is a high-frequency variable antigen that is mainly involved in MS's immune evasion [31].…”

Section: Discussionmentioning

confidence: 99%

The immunogenicity identification and adherence characterization analysis of acetate kinase of Mycoplasma synoviae

Yue

Zhang

et al. 2023

Preprint

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Background: Mycoplasma synoviae (MS) is a major pathogen that leads to a significant economic loss to the world’s industry of poultry. Acetate kinase (ACK) is a member of the key pathway for acetyl-CoA synthesis in bacteria, but its role in MS maintains unknown. Results: The immunogenicity assay and complement-dependent mycoplasmacidal assay identified recombinant protein MS ACK (rMS ACK) as an immunogenic protein. The distribution of ACK was localized to both the cytoplasm and membrane of MS detected by Western blot and ELISA. Meanwhile, adherence and adherence inhibition assay indicated that the rMS ACK could directly adhere to DF-1 cells, and anti-rMS ACK serum could effectively inhibit the adherence of MS to DF-1 cells. Conclusion: The immunogenicity and adhesion characterization of ACK membrane protein were analyzed for the first time, enhancing the understanding of the pathogenicity of MS infection.

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“…The gene family encoding these phase-and size-variable lipoproteins (Vlps) was also shown to be involved in the adhesion to host cells [23,24]. The lipoprotein VlhA, a hemagglutinin in M. synoviae, is notable as one of the most well-studied phase-variable proteins in mycoplasmas [25]. There are several other membrane-variable lipoproteins in mycoplasmas, such as the pMGA in M. gallisepticum [26], Vmm in M. mycoides subsp.…”

Section: Introductionmentioning

confidence: 99%

Predominant Single Stable VpmaV Expression in Strain GM139 and Major Differences with Mycoplasma agalactiae Type Strain PG2

Barbosa

Spergser

Marques

et al. 2022

Animals

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Although mycoplasmas have a reduced genome and no cell wall, they have important mechanisms for the antigenic variation in surface lipoproteins that modulate their interactions with the host. Mycoplasma agalactiae, the main etiological agent of contagious agalactia, has a multigene family involved in the high-frequency phase variation in surface lipoproteins called variable proteins of M. agalactiae (Vpmas). The Vpma lipoproteins are involved in the immune evasion, colonization, dissemination, and persistence of M. agalactiae in the host. In this paper, we evaluate the Vpma phenotypic profiles of two different strains of M. agalactiae, namely, GM139 and the type strain PG2, to assess possible correlations between Vpma phase variability and the geographic localization, animal origin, and pathogenicity of these two strains. Using monospecific Vpma antibodies against individual Vpmas in immunoblots, we demonstrate that, unlike PG2, which expresses six Vpma proteins with high-frequency phase variation, colonies of GM139 predominantly express VpmaV and do not exhibit any sectoring phenotype for any Vpma. Since VpmaV is one of the most important Vpmas for cell adhesion and invasion, its predominant sole expression in GM139 without high-frequency variation may be the basis of the differential pathogenicity of GM139 and PG2. Additionally, MALDI-ToF MS analysis also demonstrates significant differences between these two strains and their relatedness with other M. agalactiae strains.

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Characterization of the antigenic and functional domains of a Mycoplasma synoviae variant vlhA gene

Cited by 4 publications

References 21 publications

Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae

Evaluation of the protective efficacy of six major immunogenic proteins of Mycoplasma Synoviae

The immunogenicity identification and adherence characterization analysis of acetate kinase of Mycoplasma synoviae

Predominant Single Stable VpmaV Expression in Strain GM139 and Major Differences with Mycoplasma agalactiae Type Strain PG2

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