Characterization of the bacterial community structure of Sydney Tar Ponds sediment

William, YeungC.; WooMonica,; LeeKenneth,; GreerCharles, W

doi:10.1139/w11-032

Cited by 22 publications

(19 citation statements)

References 35 publications

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“…Community profiles were subjected to cluster analysis using Gelcompar II (Applied Maths, Sint-Marten-Latem, Belgium) with Dice's band-matching coefficient and unweighted pair group method with arithmetic averages. Individual bands were excised, reamplified, and sequenced, as described previously (82). Sequencing was performed at the Centre for Applied Genomics (The Hospital for Sick Children, Toronto, ON, Canada) with their respective primers.…”

Section: Methodsmentioning

confidence: 99%

Selective enrichment of commensal gut bacteria protects againstCitrobacter rodentium-induced colitis

Vong

Pinnell

Määttänen

et al. 2015

American Journal of Physiology-Gastrointestinal and Liver Physi

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The intestinal microbiota plays a key role in shaping the host immune system. Perturbation of gut microbial composition, termed dysbiosis, is associated with an increased susceptibility to intestinal pathogens and is a hallmark of a number of inflammatory, metabolic, and infectious diseases. The prospect of mining the commensal gut microbiota for bacterial strains that can impact immune function represents an attractive strategy to counteract dysbiosis and resulting disease. In this study, we show that selective enrichment of commensal gut lactobacilli protects against the murine pathogen Citrobacter rodentium, a well-characterized model of enteropathogenic and enterohemorrhagic Escherichia coli infection. The lactobacilli-enriched bacterial culture prevented the expansion of Gammaproteobacteria and Actinobacteria and was associated with improved indexes of epithelial barrier function (dextran flux), transmissible crypt hyperplasia, and tissue inflammatory cytokine levels. Moreover, cultivation of gut bacteria from Citrobacter rodentium-infected mice reveals the differential capacity of bacterial subsets to mobilize neutrophil oxidative burst and initiate the formation of weblike neutrophil extracellular traps. Our findings highlight the beneficial effects of a lactobacilli-enriched commensal gut microenvironment and, in the context of an intestinal barrier breach, the ability of neutrophils to immobilize both commensal and pathogenic bacteria.

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Section: Methodsmentioning

confidence: 99%

Selective enrichment of commensal gut bacteria protects againstCitrobacter rodentium-induced colitis

Vong

Pinnell

Määttänen

et al. 2015

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…PCR was performed on the extracted DNA using the bacteria-specific forward primer U341F and reverse primer U803R (formerly U758R), and the PCR products were quantified as described by Yeung et al (2011).…”

Section: Isolation Of Bacterial Species From Indoor Airmentioning

confidence: 99%

Interspecies interaction extends bacterial survival at solid–air interfaces

et al. 2013

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Despite the ubiquity of biofilms in natural and man-made environments, research on surface-associated cells has focused primarily on solid-liquid interfaces. This study evaluated the extent to which bacterial cells persist on inanimate solid-air interfaces. The desiccation tolerance of bacterial strains isolated from indoor air, as well as of a test strain (Pseudomonas aeruginosa), was determined at different levels of relative humidity (RH) using the large droplet inoculation method in an aerosol chamber. The cells survived longer at lower (25 and 42%) than at high RH (95%). Four of the seven indoor strains selected for further study showed extended period of survival following deposition as 0.05-0.1 ml of washed culture followed by desiccation, each with different effects on the survival of the test strain, P. aeruginosa. A strain closely related to Arthrobacter species afforded the highest level of protection to the test strain. Even though the desiccation-tolerant strains survived when they were deposited as bioaerosols, the protective role towards the test strain was not observed when the latter was deposited as a bioaerosol. These, which are often-unculturable, bacteria may go undetected during routine monitoring of biofouling, thereby allowing them to act as reservoirs and extending the habitat range of undesired microorganisms.

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“…The resulting sequences were aligned by comparing forward and reverse sequences using the Bioedit and checked manually [19]. Phylogenetic affiliations of individual sequences were determined based on analysis of the classifier function from the Ribosomal Database Project [41].…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

“…Recently, molecular method-based studies have indicated that bacterial diversity in sediment has been linked with impacts of anthropogenic activities, such as increase of eutrophication and chemical pollution [9][10][11][12][13], changes in species of phytoplankton or zooplankton [14][15][16], and operating a dredging, reclamation, or remediation project [17][18][19][20]. Furthermore, some efforts have been made to study the bacterial community structure dynamics related to mariculture processes.…”

Section: Introductionmentioning

confidence: 99%

Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China

et al. 2013

Microb Ecol

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Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon-Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of γ-and δ-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, γ-Proteobacteria were dominant in all samples (21.7~45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO 4 3− -P, NH 4 + -N, and Chlorophyll a content. The phyla of γ-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.

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Characterization of the bacterial community structure of Sydney Tar Ponds sediment

Cited by 22 publications

References 35 publications

Selective enrichment of commensal gut bacteria protects againstCitrobacter rodentium-induced colitis

Selective enrichment of commensal gut bacteria protects againstCitrobacter rodentium-induced colitis

Interspecies interaction extends bacterial survival at solid–air interfaces

Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China

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