Characterization of the binding capacity of mercurial species in <i>Lactobacillus</i> strains

Alcántara, Cristina; Jadán-Piedra, Carlos; Vélez, Dinoraz; Devesa, Vicenta; Zúñiga, Manuel; Monedero, Vicente

doi:10.1002/jsfa.8388

Cited by 28 publications

(22 citation statements)

References 37 publications

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“…It has been postulated that binding of heavy metals by lactic acid bacteria occurs mainly via surface interactions with cell wall components and that low proportions of the toxic are internalized through processes that may involve specific/unspecific transport or diffusion (for review, see: Mrvcic et al, 2012 ; Zoghi et al, 2014 ; Chiocchetti et al, 2018 ). This notion has been specifically proven for L. casei BL23 ( Alcantara et al, 2017 ). Since most poly-P is accumulated intracellularly as cytoplasmic granules, it would therefore have a low interaction with mercury.…”

Section: Discussionmentioning

confidence: 79%

“…Our observations indicate that the poly-P-influenced mechanisms that affect mercury resistance would not necessarily ave an impact on the capacity to accumulate mercury. It has been recently shown that lactobacilli can efficiently sequester Hg(II) as well as CH 3 Hg, the main mercurial form found as food contaminant ( Alcantara et al, 2017 ; Jadan-Piedra et al, 2017 ). However, our results question the role of poly-P in the mercury retention mechanism.…”

Section: Discussionmentioning

confidence: 99%

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Polyphosphate in Lactobacillus and Its Link to Stress Tolerance and Probiotic Properties

et al. 2018

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The synthesis of the inorganic polymer polyphosphate (poly-P) in bacteria has been linked to stress survival and to the capacity of some strains to sequester heavy metals. In addition, synthesis of poly-P by certain strains of probiotic lactobacilli has been evidenced as a probiotic mechanism due to the homeostatic properties of this compound at the intestinal epithelium. We analyzed the link between poly-P synthesis, stress response, and mercury toxicity/accumulation by comparing wild-type strains of Lactobacillus and their corresponding mutants devoid of poly-P synthesis capacity (defective in the poly-P kinase, ppk, gene). Results showed that resistance to salt (NaCl) and acidic (pH 4) stresses upon ppk mutation was affected in Lactobacillus casei, while no effect was observed in two different Lactobacillus plantarum strains. Inorganic [Hg(II)] and organic (CH3Hg) mercury toxicity was generally increased upon ppk mutation, but no influence was seen on the capacity to retain both mercurial forms by the bacteria. Notwithstanding, the culture supernatants of ppk-defective L. plantarum strains possessed a diminished capacity to induce HSP27 expression, a marker for cell protection, in cultured Caco-2 cells compared to wild-type strains. In summary, our results illustrate that the role of poly-P in stress tolerance can vary between strains and they reinforce the idea of probiotic-derived poly-P as a molecule that modulates host-signaling pathways. They also question the relevance of this polymer to the capacity to retain mercury of probiotics.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Polyphosphate in Lactobacillus and Its Link to Stress Tolerance and Probiotic Properties

et al. 2018

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“…Binding of both forms was immediate and mainly occurred at the cell wall. Cell viability was not required for binding; in fact, an increase of binding capacity was observed for heat-killed cells (Alcántara et al, 2017). Furthermore, Lactobacillus casei mutants affected in genes involved in the modulation of the negative charge of the cell wall anionic polymer lipoteichoic acid showed increased Hg biosorption.…”

Section: Metals and Metalloidsmentioning

confidence: 98%

“…Another study investigated the binding capacity of inorganic Hg [Hg(II)] and the organic form methylmercury (CH3Hg) to various strains of Lactobacillus (Alcántara et al, 2017).…”

Section: Metals and Metalloidsmentioning

confidence: 99%

Use of lactic acid bacteria and yeasts to reduce exposure to chemical food contaminants and toxicity

Chiocchetti

Jadán-Piedra

Monedero

et al. 2018

Critical Reviews in Food Science and Nutrition

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Chemical contaminants that are present in food pose a health problem and their levels are controlled by national and international food safety organizations. Despite increasing regulation, foods that exceed legal limits reach the market. In Europe, the number of notifications of chemical contamination due to pesticide residues, mycotoxins and metals is particularly high. Moreover, in many parts of the world, drinking water contains high levels of chemical contaminants owing to geogenic or anthropogenic causes. Elimination of chemical contaminants from water and especially from food is quite complex. Drastic treatments are usually required, which can modify the food matrix or involve changes in the forms of cultivation and production of the food products. These modifications often make these treatments unfeasible. In recent years, efforts have been made to develop strategies based on the use of components of natural origin to reduce the quantity of contaminants in foods and drinking water, and to reduce the quantity that reaches the bloodstream after ingestion, and thus, their toxicity. This review provides a summary of the existing literature on strategies based on the use of lactic acid bacteria or yeasts belonging to the genus Saccharomyces that are employed in food industry or for dietary purposes.

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“…Several studies have revealed that specific Lactobacillus strains alleviated heavy metal toxicity (Tian et al, 2015 ; Trinder et al, 2016 ; Yu et al, 2016 ; Alcantara et al, 2017 ). Recently, Majlesi M et al have proved the L. plantarum and Bacillus coagulans protected significantly against mercury toxicity in liver and kidney in rats by decreasing the mercury, creatinine, urea, bilirubin, ALT, and AST level, and preventing alterations in the levels of GPx and SOD (Majlesi et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus brevis 23017 Relieves Mercury Toxicity in the Colon by Modulation of Oxidative Stress and Inflammation Through the Interplay of MAPK and NF-κB Signaling Cascades

Jiang

Liu³

et al. 2018

Front. Microbiol.

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Aims: Lactobacillus strains have protective effects against heavy metals while relieving oxidative stress and modulating the immune response. Mechanisms that ameliorate heavy metal toxicity and the relationship between probiotics and gut barrier protection in the process of heavy metal pathogenesis was poorly understood.Methods and Results: In this study, Lactobacillus brevis 23017 (LAB, L. brevis 23017), a selected probiotics strain with strong mercury binding capacities, was applied to evaluate the efficiency against mercury toxicity in a mouse model. Histopathological results along with HE stains show that L. brevis 23017 protects the integrity of the small intestinal villus, which slows weight loss in response to Hg exposure. The qRT-PCR results demonstrate that L. brevis 23017 maintains a normal mucosal barrier via modulation of tight junction proteins. Importantly, the present study demonstrates that L. brevis 23017 effectively ameliorates injury of the small intestine by reducing intestinal inflammation and alleviating oxidative stress in animal models. Moreover, L. brevis 23017 blocks oxidative stress and inflammation through MAPK and NF-κB pathways, as shown by western blot.Conclusions: Together, these results reveal that L. brevis 23017 may have applications in the prevention and treatment of oral Hg exposure with fermented functional foods by protecting gut health in daily life.

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Characterization of the binding capacity of mercurial species in Lactobacillus strains

Cited by 28 publications

References 37 publications

Polyphosphate in Lactobacillus and Its Link to Stress Tolerance and Probiotic Properties

Polyphosphate in Lactobacillus and Its Link to Stress Tolerance and Probiotic Properties

Use of lactic acid bacteria and yeasts to reduce exposure to chemical food contaminants and toxicity

Lactobacillus brevis 23017 Relieves Mercury Toxicity in the Colon by Modulation of Oxidative Stress and Inflammation Through the Interplay of MAPK and NF-κB Signaling Cascades

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