Characterization of the biocontrol activity of <i>Debaryomyces hansenii</i> in the control of <i>Penicillium digitatum</i> on grapefruit

Droby, Samir; Chalutz, E.; Wilson, C. L.; Wisniewski, Michael

doi:10.1139/m89-132

Cited by 210 publications

(89 citation statements)

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“…1 and 2) or in situ by the addition of exogenous apple nutrients (Figs. 3, 4, 7 and 8), which constitute supporting evidence for a possible involvement of competition for nutrients in the biocontrol activity of strain Ach1-1 against blue mold caused by P. expansum on stored apple fruit (Droby et al, 1989;Janisiewicz et al, 2000;Castoria et al, 2001;Meziane et al, 2006). If this is the case, strain Ach1-1 applied on apple surfaces will exert a fungistatic rather than fungicidal activity on P. expansum as it will deplete (limiting) nutrients available at the infection site (wounds) and inhibit conidia germination that depends on exogenous nutrients without affecting their viability.…”

Section: Discussionmentioning

confidence: 53%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

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Aureobasidium pullulans strain Ach1-1 was selected for its effectiveness against blue mold caused by Penicillium expansum on stored apple fruit. The possible involvement of competition for nutrients in the biocontrol activity of this antagonistic strain was investigated both in vitro and in situ. For in vitro assays, the effect of strain Ach1-1 on germination percentages of P. expansum conidia was evaluated after a 24 h incubation period in the presence of increasing apple juice concentrations (0-5%) using a system allowing the physical separation of both agents. In the absence of strain Ach1-1, conidial germination was strongly promoted by apple juice whatever the concentration. However, germination was significantly reduced by the presence of strain Ach1-1 except at the highest juice concentration. For conidia previously inhibited at 0.5% juice, germination after 24 h of incubation was partially recovered in the presence of strain Ach1-1 when fresh juice was added to a final concentration of 5%, and completely restored at both 0.5 and 5% juice concentrations in the absence of strain Ach1-1. For in situ assays, strain Ach1-1 was very protective against P. expansum on postharvest wounded apples. However, the application of high concentrations of exogenous sugars, vitamins and most particularly amino acids, significantly reduced such protection. Time-course analysis of apple amino acids at the wound site revealed that these compounds were more depleted in wounds treated with strain Ach1-1 alone and especially in those treated with both agents (strain Ach1-1 and P. expansum) compared to wounds treated with P. expansum alone or to untreated ones. Exogenous amino acids, applied at high concentrations on apple wounds as a mixture of specific amino acid groups or as individuals, significantly decreased strain Ach1-1 efficacy against P. expansum. The present study provides in vitro and in situ evidence that competition for apple nutrients, most particularly amino acids, may be a main mechanism of the biocontrol activity of A. pullulans strain Ach1-1 against blue mold caused by P. expansum on harvested apple fruit.

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Section: Discussionmentioning

confidence: 53%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

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“…Several attempts have been made for the biological control of pest fungi by using antagonistic microorganisms (38)(39)(40). In these applications, the antagonist can either directly interact with the pathogen (7,41), compete with the pathogen for nutrients and space (42,43), or inhibit the pathogen by secretion of antibiotics (38,44). Several studies have focused on the antifungal secondary metabolites and enzymes produced by other fungi (27,45,46), as they appear to be a more practical and controllable alternative to the direct application of microorganisms to postharvest commodities (38).…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Arfi

Levasseur

Record

2013

Appl Environ Microbiol

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Fungi compete against each other for environmental resources. These interspecific combative interactions encompass a wide range of mechanisms. In this study, we highlight the ability of the white-rot fungus Pycnoporus coccineus to quickly overgrow or replace a wide range of competitor fungi, including the gray-mold fungus Botrytis cinerea and the brown-rot fungus Coniophora puteana. To gain a better understanding of the mechanisms deployed by P. coccineus to compete against other fungi and to assess whether common pathways are used to interact with different competitors, differential gene expression in P. coccineus during cocultivation was assessed by transcriptome sequencing and confirmed by quantitative reverse transcription-PCR analysis of a set of 15 representative genes. Compared with the pure culture, 1,343 transcripts were differentially expressed in the interaction with C. puteana and 4,253 were differentially expressed in the interaction with B. cinerea, but only 197 transcripts were overexpressed in both interactions. Overall, the results suggest that a broad array of functions is necessary for P. coccineus to replace its competitors and that different responses are elicited by the two competitors, although a portion of the mechanism is common to both. However, the functions elicited by the expression of specific transcripts appear to converge toward a limited set of roles, including detoxification of secondary metabolites. Interspecific interactions are key elements in the ecology of fungi, which live in complex communities and often have to compete against each other for a territory and the resources it contains (1, 2). Competitive mycelial interactions usually result in replacement (one of the competitors overruns the other) or deadlock (neither fungus gains any territory). The outcome of these interactions will in turn affect the succession of species, community development, and substratum decay (3-5). This ability to eliminate competitors has also been used as a biotechnological tool, with the development of methods using aggressive, strongly antagonistic species, such as Trichoderma sp., to control plantpathogenic and wood rot fungi (1, 6, 7).When antagonistic relations occur, a broad array of responses can be observed, including changes in mycelial morphology at the interaction front, modification of metabolism, and production and secretion of extracellular enzymes (1,(8)(9)(10)(11). Two main pathways are used to gain an advantage over the competitor: development of attack or defense mechanisms and improvements in nutrient uptake (12). For instance, the overproduction of a large number of oxidative enzymes, such as laccases, phenoloxidases, and peroxidases, has been demonstrated in multiple interactions (13-16). As most of these enzymes are also secreted during oxidative and abiotic stresses, it has been suggested that they are part of a defensive function that contributes to the detoxification of reactive oxygen species (ROS). Laccases and peroxidases are also thought to be involved in morpholo...

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“…An alternative to pesticide application is that, it may be possible to utilize a scheme of inducible plant defense which may provide protection against a broad spectrum of disease-causing pathogenic microorganisms. Biological control using microbial antagonists has shown potential as an alternative for natural control of plant pathogens instead of synthetic chemical fungicides [51]. Another alternative control method is given by enhancing natural resistance of plants towards the pathogen.…”

Section: Integrated Fungicidal Alternatives and Yeast (S Cerevisiae)mentioning

confidence: 99%

“…In general, biological control using microbial antagonists has shown potential as an alternative for natural control of plant pathogens instead of synthetic chemical fungicides [51]. The mode of action of antagonistic yeasts may be competition for space and nutrients [51,90], production of cell-wall lytic enzymes [91], and induction of host resistance [92,93].…”

Section: Integrated Fungicidal Alternatives and Yeast (S Cerevisiae)mentioning

confidence: 99%

“…The mode of action of antagonistic yeasts may be competition for space and nutrients [51,90], production of cell-wall lytic enzymes [91], and induction of host resistance [92,93]. In large-scale tests, the use of biological control often needed to be combined with low doses of synthetic fungicides to obtain a level of disease control equivalent to synthetic fungicides [94].…”

Section: Integrated Fungicidal Alternatives and Yeast (S Cerevisiae)mentioning

confidence: 99%

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Biological and Chemical Resistance Inducers Approaches for Controlling Foliar Diseases of Some Vegetables under Protected Cultivation System

NS¹

2013

J Plant Pathol Microb

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Characterization of the biocontrol activity of Debaryomyces hansenii in the control of Penicillium digitatum on grapefruit

Cited by 210 publications

References 0 publications

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Biological and Chemical Resistance Inducers Approaches for Controlling Foliar Diseases of Some Vegetables under Protected Cultivation System

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