Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA-1) cells

Dong, Ke; Fung, Ting; Juan, Yu Wen; Chen, Chih‐Wei; Lin, Zhiyong; Xiang, Xiaoqin; Mahony, M.C.; Gibbons, William E.; Oehninger, Sergio

doi:10.1067/mob.2001.113849

Cited by 39 publications

(15 citation statements)

References 25 publications

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“…At the present, human oocytes are required for sperm-ZP interaction test to diagnose DZPIAR and no artificial human ZP or other substitute is available to replace native human ZP. Recombinant human ZP (rhZP) proteins are easily produced by several groups but rhZP does not bind to human sperm and also vey low biological activity in inducing the AR compared with native human ZP (39)(40)(41)(42)(43)(44)(45). This is also a main reason for non progress of development of commercial rhZP kit for clinical diagnosis of male infertility with either defective sperm-ZP binding or DZPIAR.…”

Section: Figurementioning

confidence: 98%

Defective protein kinase A and C pathways are common causes of disordered zona pellucida (ZP)–induced acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding

Liu

Zhao

Baker

2013

Fertility and Sterility

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Section: Figurementioning

confidence: 98%

Defective protein kinase A and C pathways are common causes of disordered zona pellucida (ZP)–induced acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding

Liu

Zhao

Baker

2013

Fertility and Sterility

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“…There was no correlation between either calcium ionophore A23187 or progesterone-induced AR and the human ZP-induced AR (Liu and Baker, 1996a;. While recombinant human ZP3 (rhZP3) has been produced by several groups, it has little biological activity when compared with native human ZP for binding sperm or inducing the AR (van Duin, et al, 1994;Brewis, et al, 1996;Whitmarsh, et al, 1996;Dong, 2001;Martic et al, 2004). Even when rhZP1, 2 and 3 were co-expressed in a human kidney cell line to produce recombinant proteins glycosylated in a human pattern, the recombinant proteins still had no activity to bind human sperm or induce the human AR in vitro (Martic et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Hyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm

Liu

Clarke

et al. 2007

Human Reproduction

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BACKGROUND: The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro. METHODS: Sperm samples from 129 infertile men were studied. Motile sperm (2 3 10 6 ) selected by Pure sperm were incubated with four oocytes in 1 ml human tubal fluid supplemented with 10% human serum. After 2-h incubation, the number of sperm bound to the ZP and the AR of ZP-bound sperm were examined. Velocities and HA of sperm in insemination medium were assessed by Hamilton-Thorn Sperm Analyzer. RESULTS: The HA was highly correlated with the ZP-induced AR in all the subjects (rho 5 0.626, P < 0.001). In the 69 men with 100 sperm bound/ZP, allowing accurate counts, HA was not significantly correlated with sperm-ZP binding. Men with <7% HA sperm were more likely to have very low ZP-induced AR. Only normal sperm morphology was significantly correlated with sperm-ZP binding (rho 5 0.346 and 0.446 in semen and insemination medium, respectively, both P < 0.001). Sperm motility and velocities were significantly correlated with sperm morphology but not with either sperm-ZP binding or the ZP-induced AR. CONCLUSIONS: The correlation of HA with the ZP-induced AR of ZP-bound sperm suggests a mechanistic link between HA and the physiological AR in humans. Assessment of HA of capacitated sperm in vitro may be a useful clinical test for male infertility associated with defective ZP-induced AR that does not require the use of human oocytes.

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“…To circumvent this problem, human ZP proteins have been generated in vitro with use of recombinant DNA technology. In particular, ZPC has been expressed in prokaryotic and eukaryotic systems (7)(8)(9)(10)(11)(12)(13)(14). Recombinant human ZP protein C (rec-hZPC) produced in Chinese hamster ovary (CHO) cells consistently has been shown to induce the acrosomal loss of human spermatozoa (7,8,15,16); in addition, rec-hZPC has been reported to bind to sperm components in solid-phase interaction assays (17,18).…”

mentioning

confidence: 99%

Recombinant human zona pellucida protein C produced in Chinese hamster ovary cells binds to human spermatozoa and inhibits sperm–zona pellucida interaction

Marı́n-Briggiler

González-Echeverría

Harris³

et al. 2008

Fertility and Sterility

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Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA-1) cells

Cited by 39 publications

References 25 publications

Defective protein kinase A and C pathways are common causes of disordered zona pellucida (ZP)–induced acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding

Defective protein kinase A and C pathways are common causes of disordered zona pellucida (ZP)–induced acrosome reaction in normozoospermic infertile men with normal sperm-ZP binding

Hyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm

Recombinant human zona pellucida protein C produced in Chinese hamster ovary cells binds to human spermatozoa and inhibits sperm–zona pellucida interaction

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