Characterization of the biosurfactant production and enzymatic potential of bacteria isolated from an oil-contaminated saline soil

Valizadeh, Sara; Enayatizamir, Naeimeh; Ghomsheh, Habibolah Nadian; Motamedi, Hossein; Moghadam, Bijan Khalili

doi:10.1007/s10123-022-00318-w

Cited by 3 publications

(3 citation statements)

References 66 publications

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“…The potential biosurfactant and enzyme (laccase, cellulase and manganese peroxidase)‐producing bacteria were isolated from oil contaminated soil (Valizadeh et al., 2023). The screened isolates with accession numbers of ON323152, ON323150, and ON323153 were Bacillus toyonensis , Bacillus sp.…”

Section: Methodsmentioning

confidence: 99%

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Bioremediation of Crude Oil Contaminated Saline Soil Using a Bacterial Consortium and Different Carriers

Valizadeh,

Enayatizamir,

Nadian Ghomsheh

et al. 2024

JGR Biogeosciences

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Bioremediation of crude‐oil‐contaminated soils is critical to supporting soil ecosystem health and functioning. Here, to explore the potential for different remediation strategies to restore soil microbial functioning and enhance remediation, three strains of bacteria, Bacillus toyonensis, Bacillus sp. and Bacillus cereus, were inoculated to saline, crude oil‐contaminated soil collected from Azadegan Oil Field, Iran. Bacteria were added using different carriers, including corn biochar, humic acid, chitosan and kaolin, and free bacteria as a control. Changes in soil metal concentrations and residual crude oil were determined after 60 days of incubation. Soil respiration, microbial biomass, and enzyme (dehydrogenase and catalase) activities were measured. The carriers accompanying a bacterial consortium were more efficient than free bacteria in removing crude oil and heavy metals from soil, and they performed better in removing n‐alkanes with carbon chains of C12–24. The oil removal rate and metal immobilization efficiencies were greatest in soils with chitosan‐bacteria treatment, with removal of crude oil, Al, Pb, Sr, and Cr (93%, 63%, 37%, 54%, and 15%, respectively) greater than for soils with free cells without treatment (61%, 54%, 9%, 50%, and 3%, respectively). Soil electrical conductivity decreased after application of bacterial mixtures with and without carriers. The inoculation of bacteria and application of soil amendments increased soil microbial biomass carbon and the activity of catalase and dehydrogenase. Overall, our results indicated the greatest potential remediation was achieved by applying immobilized microorganisms in chitosan to accelerate the biodegradation of crude oil and the removal of heavy metals.

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Section: Methodsmentioning

confidence: 99%

“…The data used in this study are available through figshare at https://doi.org/10.6084/M9.FIGSHARE.24678900.V1 (Valizadeh et al., 2024).…”

Section: Data Availability Statementmentioning

confidence: 99%

Bioremediation of Crude Oil Contaminated Saline Soil Using a Bacterial Consortium and Different Carriers

Valizadeh,

Enayatizamir,

Nadian Ghomsheh

et al. 2024

JGR Biogeosciences

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“…It has enormous economic significance; for example, Bacillus toyonensis BCT-7112 spores have been utilized as probiotic supplements in animal nutrition [15]. It was previously reported to exhibit plant growth promotion, biodegradation, and probiotic and biocontrol properties [16][17][18]. Its biocontrol properties are rarely reported; however, Wang et al reported its toyoncin-producing ability.…”

Section: Introductionmentioning

confidence: 99%

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

El-Sayed,

Abdelaziz,

Ali

et al. 2023

Microorganisms

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Fungal infections currently pose a real threat to human lives. In the current study, soil bacterial isolates were screened for the production of antifungal compounds to combat human fungal pathogens. Notably, the bacterial F1 isolate exhibited antimycotic action towards the Candida albicans ATCC 10231 and Aspergillus niger clinical isolates. By employing phenotypic and molecular techniques, we identified the F1 isolate as the Bacillus toyonensis isolate OQ071612. The purified extract showed stability within a pH range of 6–7 and at temperatures of up to 50 °C. It demonstrated potential antifungal activity in the presence of various surfactants, detergents, and enzymes. The purified extract was identified as 6-methoxy-1H-Indole-2-carboxylic acid using advanced spectroscopic techniques. To optimize the antifungal metabolite production, we utilized response surface methodology (RSM) with a face-centered central composite design, considering nutritional and environmental variables. The optimal conditions were as follows: starch (5 g/L), peptone (5 g/L), agitation rate of 150 rpm, pH 6, and 40 °C temperature. A confirmatory experiment validated the accuracy of the optimization process, resulting in an approximately 3.49-fold increase in production. This is the first documented report on the production and characterization of 6-methoxy-1H-Indole-2-carboxylic acid (MICA) antifungal metabolite from Bacillus toyonensis.

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Nitro-PAHs: Occurrences, ecological consequences, and remediation strategies for environmental restoration

Sarma,

Gogoi,

Guan

et al. 2024

Chemosphere

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Characterization of the biosurfactant production and enzymatic potential of bacteria isolated from an oil-contaminated saline soil

Cited by 3 publications

References 66 publications

Bioremediation of Crude Oil Contaminated Saline Soil Using a Bacterial Consortium and Different Carriers

Bioremediation of Crude Oil Contaminated Saline Soil Using a Bacterial Consortium and Different Carriers

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

Nitro-PAHs: Occurrences, ecological consequences, and remediation strategies for environmental restoration

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