Characterization of the Bovine<i>κ</i>-Casein Gene Promoter

Adachi, Takahiro; Ahn, Jung-Youb; Yamamoto, Kazue; Aoki, Naohito; Nakamura, Ryô; Matsuda, Tsukasa

doi:10.1271/bbb.60.1937

Cited by 10 publications

(11 citation statements)

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“…In the only study of bovine k-casein gene promoter activity in vitro, 522 bp of promoter was tested in HC11 cells and this proximal promoter did not show higher activity than bovine b-casein gene promoter (Adachi et al 1996). Our k-casein gene promoter analysis agrees with this result, because we found the putative k-casein gene promoter enhancer element in the region between -1200 and -900 that we included in the construct K tested in the promoter activity assay.…”

Section: Discussionsupporting

confidence: 78%

“…Despite the important role of k-casein in casein micelle formation, markedly fewer experiments have been performed with k-casein gene promoters (Rijnkels et al 1995;Adachi et al 1996) owing to lower expression levels in vivo of k-casein gene compared with b-casein gene. Several experiments in vitro and in vivo have examined the activation of transcription from mouse, rat, rabbit and bovine b-casein gene promoters.…”

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confidence: 99%

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Functional study of the equine β-casein and κ-casein gene promoters

Lenasi

Kokalj-Vokac²,

Narat

et al. 2005

Journal of Dairy Research

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Casein genes are expressed in a tissue-specific and highly coordinated manner. The main goals of casein gene promoter studies are to unravel cis-and trans-acting factors involved in the complex signalling pathway controlling milk production, and to explore the possibility of using these promoters for tissue-specific production of heterologous proteins in the mammary gland.Here we present a comparative study of the equine b-casein and k-casein gene proximal promoters. In order to confirm the assumption that in the horse, as in other mammalian species, casein genes are organized in a cluster located on a single chromosome, we performed in situ hybridization of pro-metaphase chromosomes with two BAC clones containing different equine casein genes. Sequence analysis of the b-casein and k-casein gene proximal promoters revealed binding sites for activators (STAT5, GRE, NF1, MAF) and repressors (YY1, PMF), characteristic for casein genes. The alignments of casein gene promoters revealed the highest sequence identity in the proximal promoter region between the equine and human b-casein gene promoters. We directly compared the activity of equine b-casein and k-casein gene promoters in vitro using bovine mammary gland cell line BME-UV1. In this system, the k-casein gene proximal promoter activated the reporter gene expression more efficiently than the b-casein gene promoter of approximately the same length. The 810 bp of b-casein promoter activated the reporter gene expression more efficiently than the long fragment (1920 bp) and the 1206 bp fragment of the same promoter, which included also 396 bp of 5k UTR.

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Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 99%

Functional study of the equine β-casein and κ-casein gene promoters

Lenasi

Kokalj-Vokac²,

Narat

et al. 2005

Journal of Dairy Research

View full text Add to dashboard Cite

show abstract

“…The most important part of this region necessary for expression was limited to -552 (Adachi et al, 1996). The entire 5' flanking region contains experimentally confirmed or computationally deduced common as well as specific DNA motifs engaged in regulation of CASK gene transcription (Groenen and van der Poel, 1994;Adachi et al, 1996;Malewski, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The choice of Dde I polymorphism in position -385 within the CASK promoter was made for two reasons: 1. this mutation is inside the regulatory element of a 132 bp fragment (-439/-308) which was defined by deletion mutation analysis in a mammary epithelial cell line as preferentially expressed during pregnancy (Adachi et al, 1996); 2. within the amplified 214 bp CASK promoter fragment, three point mutations were reported by Schild et al (1994). Dde I polymorphism refers to point mutation (C/T) in position -385 which is located within the recognition site for the AP-2 transcription factor.…”

Section: Discussionmentioning

confidence: 99%

“…Cows with MM genotype were not in cluded in the analysis, because of too small number of animals (only 2 cows). The sequence between nucleotide -439 and -308 refers to regulatory element of CASK promoter shown to be preferentially expressed during pregnancy (Adachi et al, 1996). In the left side of the figure, the example of CASK-R genotyping by PCR-RFLP method is shown.…”

Section: Discussionmentioning

confidence: 99%

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