Characterization of the Bovine Prolactin Gene

Camper, Sally A.; Luck, Dennis N.; Yao, Yvonne; Woychik, Richard P.; Goodwin, Ray G.; Lyons, Robert H.; Rottman, Fritz M.

doi:10.1089/dna.1.1984.3.237

Cited by 69 publications

(34 citation statements)

References 53 publications

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“…Therefore, the PRL gene is potentially a strong genetic marker for the improvement of livestock. The gene consists of 5 exons and 4 introns, and is 10 kb in size, encoding the 199 amino acid in the BTA23 (Camper et al, 1984;Freeman et al, 2000;Dybus, 2002). PRL is secreted from the pituitary gland and cells, mainly the lymphocytes, and has an immunostimulatory effect and promotes autoimmunity (Orbach & Shoenfeld, 2007).…”

Section: Introductionmentioning

confidence: 99%

Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey

Sönmez¹,

Özdemir²

2017

SA J. An. Sci.

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Prolactin (PRL) plays an important role in regulating mammary gland development, secreting milk, and expressing milk protein genes; making it a potential genetic marker and a candidate gene for production traits in dairy animals. The aim of the study was to determine by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method the gene and genotype frequencies of PRL gene in native East Anatolian Red (EAR) cattle, which are raised as a genetic resource in Turkey. PCR-RFLP analysis involved the use of the RsaI restriction enzyme. Three patterns of fragments were obtained. The AA, AG, and GG genotype frequencies were 0.07, 0.34, and 0.59 in the cattle population, respectively. For Prolactin-RsaI (PRL-RsaI) polymorphism, the population was in Hardy-Weinberg equilibrium. Heterozygosity was found at a medium rate as 0.338 and the calculated F IS value was 0.072.

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Section: Introductionmentioning

confidence: 99%

Prolactin-RsaI gene polymorphism in East Anatolian Red cattle in Turkey

Sönmez¹,

Özdemir²

2017

SA J. An. Sci.

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“…Hence, PRL gene is a potential quantitative trait locus and genetic marker of production traits in dairy cattle (Brym et al, 2005). Bovine PRL gene has been located on chromosome 23 and is composed of five exons and four introns with an overall length of approximately 10 kb encoding the 199 amino-acid mature protein (Camper et al, 1984). A silent A→G transition mutation at the codon for amino acid 103 in exon-3 of size 156 bp bovine PRL gene gives rise to a polymorphic Rsa I (Rhodopseudomonas sphaeroides).…”

Section: Introductionmentioning

confidence: 99%

Prolactin gene polymorphism and its associations with milk production traits in Frieswal cow

Bukhari¹,

Khan²,

Gupta³

et al. 2013

ijmz

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In the present study, polymorphism of PRL gene at exon-3 and its association with milk production traits inFrieswal cattle was investigated. Prolactin (PRL) gene exerts multiple effects on the mammary gland include mammogenesis, lactogenesis and galactopoiesis. In order to evaluate the PRL gene polymorphism, the restriction fragment length polymorphism (RFLP) method was employed in this research. Blood samples were collected from randomly chosen 54 Frieswal lactating cows. Genomic DNA was extracted from venous blood by the method of John et al (1991) with slight modification and amplified by polymerase chain reaction technique. The PRL gene of the Frieswal cattle was amplified to generate a 156 bp fragment. The PCR products were electrophoresed on 2% agarose gel and stained by ethidium bromide. Then they were digested of amplicons with Rsa I, which revealed two alleles A and B. Data were analyzed by using Pop GenePopgene32 software package and association was analyzed by simple analysis of variance model. In this population, AA, AB, and BB genotypes were identified with 0.315, 0.629 and 0.056 frequencies, respectively. Allele frequencies of A and B were 0.630 and 0.370, respectively. The significant (P<0.05) chi-square value in Frieswal cattle breeds showed that the studied population was not in Hardy-Weinberg equilibrium. It is concluded from the results of present study that the animals of BB genotype for higher lactation length and AB genotype for lactation yield and animals of AA genotype for minimum service period may be selected for future breeding.

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“…The bPRL gene is about 10 kb in size and is composed of 5 exons and 4 introns (Camper et al, 1984). This encodes the 199 amino acids mature protein in cattle (Cooke et al, 1981).…”

Section: Bovine Prolactin Gene Structurementioning

confidence: 99%