Characterization of the CCR3 and CCR9 genes in miiuy croaker and different selection pressures imposed on different domains between mammals and teleosts

Zhu, Zhihuang; Wang, Rixin; Ren, Liping; Xu, Tianjun

doi:10.1016/j.dci.2013.06.015

Cited by 43 publications

(19 citation statements)

References 66 publications

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“…CCR9, an orphan receptor previously named GPR-9-6, is specific for thymus-expressed chemokine (TECK) or CCL25 [20,21]. CCR9 signaling pathway plays a role in immature T cell survival through PI3K and Ga i protein- Fig.…”

Section: Discussionmentioning

confidence: 99%

CCR9–CCL25 interaction suppresses apoptosis of lung cancer cells by activating the PI3K/Akt pathway

Wang

Zhong

et al. 2015

Med Oncol

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CC chemokine receptor-9 (CCR9) is highly expressed in non-small cell lung cancer (NSCLC) tissues and cell lines. However, the biological functions and the signals elicited by the interaction between CCR9 and its natural ligand CCL25 in NSCLC are unknown. Here, we selectively depleted CCR9 and inhibited CCR9-CCL25 interaction in NSCLC cells using small recombinant lentivirus-mediated miRNA, and investigated the tumorigenic effects in vitro and in vivo. Compromised CCR9-CCL25 interaction promoted apoptosis in NSCLC cells by activating phosphoinositide 3-kinase (PI3K)/Akt in vitro. In addition, we showed that CCR9-CCL25 interaction mediated the activation of the PI3K/Akt pathway in NSCLC cells, resulting in the up-regulation of anti-apoptotic proteins, as well as the down-regulation of apoptotic proteins in a PI3K-/Akt-dependent manner. These CCR9-CCL25-mediated effects were abrogated in the presence of a PI3K inhibitor (wortmannin 10 nM) or by inhibiting the CCR9-CCL25 interaction through CCR9 silencing, which also suggested that the biological function of CCR9-CCL25 was mainly regulated by PI3K. In vivo studies also demonstrated a significantly lower tumor burden in mice receiving CCR9-silence cells than those in mice receiving control cells. Together, these data suggested that CCR9-CCL25 interaction induced tumorigenesis of NSCLC cells and that this induction might be accomplished through the activation of the PI3K/Akt pathway. These findings may lead to a better understanding of the biological effects of CCR9-CCL25 interaction and provide clues for identifying novel therapeutic and preventive molecular markers for NSCLC.

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Section: Discussionmentioning

confidence: 99%

CCR9–CCL25 interaction suppresses apoptosis of lung cancer cells by activating the PI3K/Akt pathway

Wang

Zhong

et al. 2015

Med Oncol

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“…Fish were acclimatized in a recirculating seawater system at an ambient temperature (25°C) for one week. Challenge experiments, as previously described by Zhu et al (2013), were performed on the miiuy croaker. Fish were randomly divided into two groups, namely, injection and control groups.…”

Section: Fish Sampling and Challenge Experimentsmentioning

confidence: 99%

Evolution of akirin family in gene and genome levels and coexpressed patterns among family members and rel gene in croaker

Liu

Gao

2015

Developmental & Comparative Immunology

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“…The infected health fish were killed at 6 h, 12 h, 24 h, 36 h, 48 h, and 72 h, respectively. Three immune tissues (liver, spleen and kidney) samples of three individuals were collected, then frozen immediately in liquid nitrogen and stored at À80 C until RNA isolation, as well as the ten tissues removed from the healthy fish [32].…”

Section: Sample Preparation and Bacterial Challengementioning

confidence: 99%

Comparative genomic of the teleost cathepsin B and H and involvement in bacterial induced immunity of miiuy croaker

Che

Wang

2014

Fish & Shellfish Immunology

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Characterization of the CCR3 and CCR9 genes in miiuy croaker and different selection pressures imposed on different domains between mammals and teleosts

Cited by 43 publications

References 66 publications

CCR9–CCL25 interaction suppresses apoptosis of lung cancer cells by activating the PI3K/Akt pathway

CCR9–CCL25 interaction suppresses apoptosis of lung cancer cells by activating the PI3K/Akt pathway

Evolution of akirin family in gene and genome levels and coexpressed patterns among family members and rel gene in croaker

Comparative genomic of the teleost cathepsin B and H and involvement in bacterial induced immunity of miiuy croaker

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