Characterization of the chemical constituents in Hongjingtian injection by liquid chromatography quadrupole time‐of‐flight mass spectrometry

Liu, Yining; Chen, Canhui; Qiu, Jiuwen; Fang, Zongbao; Wu, Haibo; Zhang, Xingxian; Wang, Shufang

doi:10.1002/bmc.4446

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…The characteristic fragments were [M–H–C 2 H 4 ] - ( m/z 169.0138) and [M–H–C 2 H 4 –CO 2 ] - ( m/z 125.0239). Comparison with literature data [ 25 ] and library alignment ( Fig. S4B ; score: 94) revealed that compound 31 was ethyl gallate, a component of Rhodiolae Crenulatae Radix Et Rhizoma .…”

Section: Resultsmentioning

confidence: 91%

“…In total, 104 compounds, including alkaloids, flavonoids, phenols, phenolic acids, phenylpropanoids, quinones, terpenoids, and other phytochemicals, were putatively identified (as listed in Table 1 [ [16] , [17] , [18] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] ]) using the library alignment method. According to previous studies [ [16] , [17] , [18] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] ], the identified compounds are present in the herbs used in the LHQW formulation. No phytochemical was identified from Dryopteridis Crassirhizomatis Rhizoma .…”

Section: Resultsmentioning

confidence: 99%

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Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

Cheng

Deng

et al. 2021

Journal of Pharmaceutical Analysis

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The Lianhua Qingwen (LHQW) capsule is a popular traditional Chinese medicine for the treatment of viral respiratory diseases. In particular, it has been recently prescribed to treat infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, due to its complex composition, little attention has been directed toward the analysis of chemical constituents present in the LHQW capsule. This study presents a reliable and comprehensive approach to characterizing the chemical constituents present in LHQW by high-performance liquid chromatography-Q Exactive-Orbitrap mass spectrometry (HPLC-Q Exactive-Orbitrap-MS) coupled with gas chromatography-mass spectrometry (GC-MS). An automated library alignment method with a high mass accuracy (within 5 ppm) was used for the rapid identification of compounds. A total of 104 compounds, consisting of alkaloids, flavonoids, phenols, phenolic acids, phenylpropanoids, quinones, terpenoids, and other phytochemicals, were successfully characterized. In addition, the fragmentation pathways and characteristic fragments of some representative compounds were elucidated. GC-MS analysis was conducted to characterize the volatile compounds present in LHQW. In total, 17 compounds were putatively characterized by comparing the acquired data with that from the NIST library. The major constituent was menthol, and all the other compounds were terpenoids. This is the first comprehensive report on the identification of the major chemical constituents present in the LHQW capsule by HPLC-Q Exactive-Orbitrap-MS, coupled with GC-MS, and the results of this study can be used for the quality control and standardization of LHQW capsules.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

Cheng

Deng

et al. 2021

Journal of Pharmaceutical Analysis

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“…R. crenulata is the official species listed in the Chinese Pharmacopoeia (version of 2020), while some other species are also commonly used, such as R. wallichiana, R. angusta, and R. sachalinensis [65,66]. Specifically, R. wallichiana is the only species that has been developed to injection for treating stable angina pectoris associated with coronary heart disease [67]. In light of the diverse functions of Rhodiola, more and more products have been developed.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, R . wallichiana is the only species that has been developed to injection for treating stable angina pectoris associated with coronary heart disease [ 67 ]. In light of the diverse functions of Rhodiola , more and more products have been developed.…”

Section: Discussionmentioning

confidence: 99%

Quality Evaluation of Randomized Controlled Trials of Rhodiola Species: A Systematic Review

Chen

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Rhodiola is a worldwide used medicinal plant for its various medicinal functions, and the number of randomized controlled trials (RCTs) of Rhodiola is increasing in recent years. This study aims to evaluate the reporting quality and risk of bias of the current RCT reports of different Rhodiola species. Methods. Six databases including Embase, PubMed, Web of Science, the Cochrane Library, ClinicalTrial.gov, and China National Knowledge Infrastructure were searched to identify RCTs that used Rhodiola as a single intervention and were published in English or Chinese from inception to December 2020. The Consolidated Standards of Reporting Trials (CONSORT) 2010 statement was used as the checklist for assessment, and a scoring system was applied to the evaluation of RCTs. Score 0 represents no reporting or inadequate reporting, and score 1 represents adequate reporting. The risk of bias of the included studies was also assessed using the Cochrane Risk of Bias tool. Results. A total of 39 RCTs were included in this study, including 23 RCTs of Rhodiola rosea (R. rosea), 8 RCTs of Rhodiola crenulata (R. crenulata), and 8 RCTs of Rhodiola wallichiana (R. wallichiana). None of the included studies met all the CONSORT statement criteria, and the reporting quality of RCTs of the three Rhodiola species was all generally poor. Based on the risk of bias assessment, the majority of included studies were judged to have an unclear risk of bias in most domains due to inadequate reporting. Conclusions. There is inadequate reporting among the included RCTs of different Rhodiola species, and RCTs of Rhodiola with higher reporting quality and better methodological quality are needed.

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“…The chemical constituents of RWC injection (RWCI) have been comprehensively studied. On the one hand, a total of 49 compounds including 10 organic acids, 9 phenylethanoids, 10 phenylpropanoids, 2 flavonoid glycosides, 7 monoterpene glycosides, 7 octylglycosides, and 4 other types of compounds were identified using the ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) analysis method [14]. On the other hand, the fingerprint RWCI had been established by HPLC [15].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

Ran

Pang

Guan

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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In traditional Chinese medicine theory, blood stasis syndrome (BSS), characterized by blood flow retardation and blood stagnation, is one of the main pathologic mechanisms and clinical syndromes of cardiovascular diseases (CVDs). Rhodiola wallichiana var. cholaensis injection (RWCI) is made from dry roots and stems of RWC via the processes of decoction, alcohol precipitation, filtration, and dilution. Studies indicated the extracts of RWC could alleviate CVDs; however, the mechanism had not been illustrated. In the present study, the acute blood stasis rat model was established to investigate the pathogenesis of BSS and the therapeutic mechanism of RWCI against BSS. Hemorheological parameters (whole blood viscosity and plasma viscosity) and inflammatory factors (TNF-α and IL-6) were used to evaluate the success of the BSS rat model and RWCI efficacy. 14 and 33 differential metabolites were identified from plasma and urine samples using the metabolomics approach based on ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. The results of multivariate analysis displayed that there were significant separations among model, control, and treatment groups, but the high-dose RWCI treatment group was closer to the control group. 9 perturbed metabolic pathways were related to BSS's development and RWCI intervention. 5 metabolic pathways (arachidonic acid metabolism, linoleic acid metabolism, alpha-linolenic acid metabolism, retinol metabolism, and steroid hormone biosynthesis) showed apparent correlations. These differential metabolites and perturbed metabolic pathways might provide a novel view to understand the pathogenesis of BSS and the pharmacological mechanism of RWCI.

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Characterization of the chemical constituents in Hongjingtian injection by liquid chromatography quadrupole time‐of‐flight mass spectrometry

Cited by 14 publications

References 14 publications

Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

Quality Evaluation of Randomized Controlled Trials of Rhodiola Species: A Systematic Review

Therapeutic Effect and Mechanism Study of Rhodiola wallichiana var. cholaensis Injection to Acute Blood Stasis Using Metabolomics Based on UPLC-Q/TOF-MS

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